Active Galectin 2 (GAL2)
LGALS2; HL14; Lectin,Galactoside-Binding Soluble 2; Beta-galactoside-binding lectin L-14-II; Lactose-binding lectin 2; S-Lac lectin 2
- Product No.APA302Ra01
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- Traits Freeze-dried powder
- Purity> 97%
- Isoelectric Point7.7
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
- FOB
US$ 331
For more details, please contact local distributors! US$ 828 US$ 1656 US$ 4968 US$ 12420
ACTIVITY TEST
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Galectin 2 (GAL2) belongs to the proto type group and consists of two homologous carbohydrate recognition domains (CRDs) resulting in multiple sugar binding sites. The expression of gal-2 has been shown to be involved in processes of angiogenesis and inflammation but was not analyzed before in preeclamptic (PE) placentas.It also can agglutinate red blood. In this case, chose rabbit erythrocyte (RaE) to assay its ability of agglutination. A general procedure for hemagglutination assay (or haemagglutination assay; HA) is as follows, two-fold dilute the recombinant rat GAL2 with 0.9% sodium chloride injection, add 50μL a serial dilution of GAL2 to each well of a U or V-bottom shaped 96-well microtiter plate. The final well serves as a negative control without GAL2, replace with 50μL 0.9% sodium chloride injection. Then add 50μL 1% rabbit erythrocyte to each well and mixed gently. The plate is incubated for 3 hours at room temperature. The results are shown in Figure 1. It was obvious that the minimal effective concentration of GAL2 is 1.25μg/ml.
![](/upload/product/pictures/APA302Ra01-202301171542143312.jpg)
Figure 2. The hemagglutination assay of GAL2 in V- bottom shaped 96-well microtiter plate.
USAGE
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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Magazine | Citations |
Biol Pharm Bull | Potential Interaction between Galectin-2 and MUC5AC in Mouse Gastric Mucus Pubmed: 32009121 |
International Journal of Molecular Sciences | Galectin-2 Has Bactericidal Effects against Helicobacter pylori in a β-galactoside-Dependent Manner Pubmed: 32295066 |
Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
APA302Ra01 | Active Galectin 2 (GAL2) | Cell culture; Activity Assays. |
RPA302Ra01 | Recombinant Galectin 2 (GAL2) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA302Ra01 | Polyclonal Antibody to Galectin 2 (GAL2) | WB; IHC; ICC; IP. |