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Active Transforming Growth Factor Beta 2 (TGFb2)

TGF-B2; TGF-Beta2; G-TSF; LAP; Cetermin; Polyergin; Latency-associated peptide; BSC-1 cell growth inhibitor; Glioblastoma-derived T-cell suppressor factor

ACTIVITY TEST

Transforming growth factor beta (TGF-β) is a multifunctional cytokine belonging to the transforming growth factor superfamily. The TGF-β superfamily includes endogenous growth inhibiting proteins; an increase in expression of TGF-β often correlates with the malignancy of many cancers and a defect in the cellular growth inhibition response to TGF-β. Its immunosuppressive functions then come to dominate, contributing to oncogenesis. To test the effect of TGF-β on inhibit HGF-dependent proliferation, HepG2 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach, replaced with serum-free overnight, then the medium was replaced with 2% serum standard DMEM including 1ng/mL HGF prior to the addition of various concentrations of recombinant chicken TGF-β. After incubated for 96h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37℃. The inhibitory effect of TGF-β on HGF-dependent proliferation of HepG2 cells observed by inverted microscope was shown in Figure1. Cell viability was assessed by CCK-8 assay after incubation with recombinant TGF-β for 96h. The result was shown in Figure 2. It was obvious that TGF-β significantly decreased cell viability of HepG2 cells. (A) HepG2 cells cultured in DMEM, stimulated with 1µg/mL TGF-β for 96h; (B) Unstimulated HepG2 cells cultured in DMEM for 96h.
Figure. The inhibitory effect of TGF-β on cell proliferation of HepG2 cells.

Figure. TGF-β inhibit cell proliferation of HepG2 cells.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
international Journal of food and nutritional sciences EVALUATION OF PHYSICO-CHEMICAL PROPERTIES OF COLOSTRUM SUPPLEMENTED DAHI Ijfans: Source
J Reprod Immunol The immunomodulating effect of seminal plasma on T cells PubMed: 25799173
International Journal of Chemical Studies Studies on quality attributes of skimmed colostrum powder P-ISSN: 2349–8528
JCI Insight Angiocrine signals regulate quiescence and therapy resistance in bone metastasis Pubmed: 31292293
Mol Med Association of CASC18/miR-20a-3p/TGFB2 ceRNA axis with occult lymph node metastasis in tongue squamous cell carcinoma 34362313
Food Chem Effect of convection and microwave heating on the retention of bioactive components in human milk 34896952
Catalog No. Related products for research use of Chicken (Gallus) Organism species Applications (RESEARCH USE ONLY!)
APA218Ga01 Active Transforming Growth Factor Beta 2 (TGFb2) Cell culture; Activity Assays.
RPA218Ga01 Recombinant Transforming Growth Factor Beta 2 (TGFb2) Positive Control; Immunogen; SDS-PAGE; WB.
PAA218Ga01 Polyclonal Antibody to Transforming Growth Factor Beta 2 (TGFb2) WB; IHC; ICC; IP.