Active Thrombopoietin (TPO)

THPO; MGDF; MKCSF; ML; MPLLG; Myeloproliferative Leukemia Virus Oncogene Ligand; Megakaryocyte Growth And Development Factor; Megakaryocyte Stimulating Factor

ACTIVITY TEST

Thrombopoietin (TPO), is a key regulator of megakaryocytopoiesis and thrombopoiesis. It is principally produced in the liver and is bound and internalized by the receptor Tpo R/c-mpl. Defects in the Tpo-Tpo R signaling pathway are associated with a variety of platelet disorders. The activity of recombinant mouse TPO was measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. MO7e cells were seeded into triplicate wells of 96-well plates at a density of 30,000 cells/well in RPMI-1640 with the addition of various concentrations of rmTPO. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 µl of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 °C. Cell proliferation of MO7e cells after incubation with rmTPO for 72h observed by inverted microscope was shown in Figure 1. The dose-effect curve of rmTPO was shown in Figure 2. It was obvious that rmTPO significantly promoted cell proliferation of MO7e cells .The EC50 for this effect is typically 0.26 ug/ml.

Figure 2. The dose-effect curve of rmTPO on MO7e cells

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
中国药理学通报 黄芪和当归的主要活性成分配伍对骨髓抑制小鼠造血功能的影响
Vaccine The effect of ChAdOx1 nCov-19 vaccine on arterial thrombosis development and platelet aggregation in female rats Pubmed:35183388
Asian Journal of Transfusion Science A cross-sectional study on thrombopoietin levels in immune thrombocytopenia and its correlation with platelet count, megakaryocytes, and treatment response
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPA135Mu01 Recombinant Thrombopoietin (TPO) Positive Control; Immunogen; SDS-PAGE; WB.
APA135Mu61 Active Thrombopoietin (TPO) Cell culture; Activity Assays.
RPA135Mu02 Recombinant Thrombopoietin (TPO) Positive Control; Immunogen; SDS-PAGE; WB.
APA135Mu01 Active Thrombopoietin (TPO) Cell culture; Activity Assays.
PAA135Mu01 Polyclonal Antibody to Thrombopoietin (TPO) WB; IHC; ICC; IP.
PAA135Mu02 Polyclonal Antibody to Thrombopoietin (TPO) WB; IHC; ICC; IP.
SEA135Mu ELISA Kit for Thrombopoietin (TPO) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA135Mu Multiplex Assay Kit for Thrombopoietin (TPO) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.