≡

Active Oncostatin M (OSM)

OS-M

ACTIVITY TEST

Oncostatin M (OSM) is an approximately 30 KDa secreted cytokine belonging to the Interleukin-6 family. Like other members of the IL-6 family such as IL-11, CNTF, and Cardiotrophin-1, OSM plays crucial roles in inflammation, neuroprotection, hematopoiesis, metabolism and development. To test the effect of OSM on cell proliferation, TF-1 cells were seeded into triplicate wells of 96-well plates at a density of 20,000 cells/well with various concentrations of recombinant human OSM. After incubated for 72 hours, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8(CCK-8). Briefly, 10 µl of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37℃. Proliferation of TF-1 cells after incubation with OSM for 72 hours observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant human OSM for 72 hours. The result was shown in Figure 2. It was obvious that recombinant human OSM significantly increased cell viability of TF-1 cells, the EC50 was 0.08 ng/ml.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Journal of Obstetrics and Gynaecology Research Oncostatin M as a target biological molecule of preeclampsia PubMed: 20149034
J Neuroinflammation Oncostatin M promotes excitotoxicity by inhibiting glutamate uptake in astrocytes: implications in HIV-associated neurotoxicity via Smad2/4 in Human Renal Proximal Tubular Epithelial Cells Pubmed:27287400
22 Modulating the phenotype of host macrophages to enhance osteogenesis in MSC-laden hydrogels: Design of a glucomannan coating material. pubmed:28582717
Advanced Functional Materials Fungal Component Coating Enhances Titanium Implant‐Bone Integration Doi: 10.1002/adfm.201804483
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
EPA110Hu61 Eukaryotic Oncostatin M (OSM) Positive Control; Immunogen; SDS-PAGE; WB.
RPA110Hu01 Recombinant Oncostatin M (OSM) Positive Control; Immunogen; SDS-PAGE; WB.
APA110Hu61 Active Oncostatin M (OSM) Cell culture; Activity Assays.
APA110Hu01 Active Oncostatin M (OSM) Cell culture; Activity Assays.
PAA110Hu06 Polyclonal Antibody to Oncostatin M (OSM) WB; IHC; ICC; IP.
PAA110Hu01 Polyclonal Antibody to Oncostatin M (OSM) WB; IHC; ICC; IP.
MAA110Hu22 Monoclonal Antibody to Oncostatin M (OSM) WB; IHC; ICC; IP.
SEA110Hu ELISA Kit for Oncostatin M (OSM) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA110Hu CLIA Kit for Oncostatin M (OSM) Chemiluminescent immunoassay for Antigen Detection.
LMA110Hu Multiplex Assay Kit for Oncostatin M (OSM) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA110Hu01 ELISA Kit DIY Materials for Oncostatin M (OSM) Main materials for "Do It (ELISA Kit) Yourself".