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Active Macrophage Inflammatory Protein 1 Beta (MIP1b)
CCL4; ACT2; G-26; LAG1; MIP1-B; SCYA4; Chemokine C-C-Motif Ligand 4; Small Inducible Cytokine A4; Homologous To Mouse Mip-1b
- Product No.APA093Hu61
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 5% Trehalose.
- Traits Freeze-dried powder
- Purity> 95%
- Isoelectric Point4.5
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
- FOB
US$ 220
US$ 550
US$ 1100
US$ 3300
US$ 8250
For more details, please contact local distributors!
ACTIVITY TEST
Macrophage Inflammatory Protein 1 Beta (MIP‑1β, A093Hu), also known as CCL4, is a small pro‑inflammatory CC chemokine mainly secreted by macrophages, monocytes, dendritic cells and activated lymphocytes. It participates in immune surveillance, inflammatory response and host defense against pathogens by recruiting and activating immune cells. MIP‑1β is widely involved in infectious diseases, autoimmune disorders and tumor‑related immune infiltration. It binds to the chemokine receptor CCR7 (B647Mu) with high affinity, mediating immune cell chemotaxis and migration. Briefly, CCR7 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μL were then transferred to MIP‑1β-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-CCR7 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630 nm immediately. Measured by its binding ability in a functional ELISA. When recombinant human MIP‑1β is lmmobilized at 2 µg/mL(100 µL well), the concentration of CCR7 that produces 50% optimal bindingresponse is found to be approximately 0.154 µg/mL.
USAGE
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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| Magazine | Citations |
| Inhalation Toxicology | Age influence on hypersensitivity pneumonitis induced in mice by exposure to Pantoea agglomerans Pubmed: 24044680 |
| PLoS One. | Megakaryocytic leukemia 1 (MKL1) regulates hypoxia induced pulmonary hypertension in rats. Pubmed: 24647044 |
| PLOS ONE | Megakaryocytic Leukemia 1 (MKL1) Regulates Hypoxia Induced Pulmonary Hypertension in Rats Pubmed:Pmc3960100 |
| Cell Physiol Biochem | MicroRNA-155 Promotes Atherosclerosis Inflammation via Targeting SOCS1 PubMed: 26159489 |
| Am J Alzheimers Dis Other Demen. | Tong Luo Jiu Nao, a Chinese Medicine Formula, Reduces Inflammatory Stress in a Mouse Model of Alzheimer's Disease Pubmed:26993489 |
| Pharmacological Reports | The Association between Inflammatory Markers (iNOS, HO-1, IL-33, MIP-1β) and Depression with and without Posttraumatic Stress Disorder 10.1016:j.pharep.2018.06.001 |
| Metabolic Brain Disease | Neuroprotective effects of Bacopa monnieri in Parkinson's disease model Pubmed: 31834548 |