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Active Leukemia Inhibitory Factor (LIF)

CDF; D-FACTOR; HILDA; MLPLI; Cholinergic Differentiation Factor; Differentiation-Stimulating Factor; Melanoma-Derived LPL Inhibitor; Emfilermin

ACTIVITY TEST

Leukemia Inhibitory Factor (LIF) is a multifunctional cytokine belonging to the interleukin-6 (IL-6) family. First identified in 1987 for its ability to inhibit the proliferation of myeloid leukemia cells and induce their differentiation, LIF is now recognized for broader biological roles. It is produced by various cell types, including fibroblasts, endothelial cells, and immune cells like T lymphocytes and macrophages. Beyond hematology, LIF plays critical roles in embryonic development—supporting the self-renewal of embryonic stem cells in mice—and tissue repair, inflammation regulation, and neural function. Its signaling occurs via binding to a heterodimeric receptor complex, activating downstream pathways like JAK-STAT and MAPK. Dysregulated LIF expression has been linked to diseases such as cancer, autoimmune disorders, and neurodegenerative conditions, making it a potential therapeutic target.Besides,IL5RA has been identified as an interactor of LIF, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human LIF and recombinant human IL5RA.Briefly, biotin-linked LIF were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100µl were then transferred to IL5RA-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µl stop solution to the wells and read at 450nm immediately. The binding activity of LIF and IL5RA was shown in Figure 1, the EC50 for this effect is 0.37163µg/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
British Journal of Anaesthesia Leukemia inhibitory factor (LIF) potentiates antinociception activity and inhibits tolerance induction of opioids pubmed:28077540
Biological Rhythm Research A study of altered cytokine rhythms associated with successful implantation in cows 10.1080/09291016.2017.1361159
Integrated analysis of cancer stem cells-associated lncRNA-miRNA-mRNA network for ovarian cancer via microarray and Gene Expression Omnibus database
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EPA085Bo61 Eukaryotic Leukemia Inhibitory Factor (LIF) Positive Control; Immunogen; SDS-PAGE; WB.
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RPA085Bo01 Recombinant Leukemia Inhibitory Factor (LIF) Positive Control; Immunogen; SDS-PAGE; WB.
PAA085Bo01 Polyclonal Antibody to Leukemia Inhibitory Factor (LIF) WB; IHC; ICC; IP.
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SEA085Bo ELISA Kit for Leukemia Inhibitory Factor (LIF) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA085Bo Multiplex Assay Kit for Leukemia Inhibitory Factor (LIF) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.