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Active Interleukin 18 (IL18)

IGIF; IL-1g; IL1F4; IL1-F4; Interferon-Gamma-Inducing Factor; Interleukin-1 Family Member 4; Iboctadekin; Interleukin-1 Gamma

ACTIVITY TEST

IL18 (interleukin 18) is a cytokine that shares similarities in structure with IL1, and is produced by macrophages and other cells. IL18 has been reported to stimulate T cells proliferation and NK cell activity, besides, murine IL18 has been found to enchance the production of INFγ in spleen cells in vitro. Thus, a stimulation assay was conducted to detect the activity of IL18 using murine spleen cells. Briefly, murine spleen cells were seeded into wells of 6-well plates at a density of 2×106 cells/mL in RPMI-1640 with the addition of various concentrations of IL18. After incubation for 48 hours, the concentration of INFγ in the cell supernatant was detected using an ELISA kit. INFγ levels in the cell supernatant of spleen cells increased significantly after stimulated with IL18, the data was shown in Figure 1.

USAGE

Reconstitute in ddH2O to a concentration less than or equal to 0.1mg/mL. Do not

vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
International Immunopharmacology Curcumin immune-mediated and anti-apoptotic mechanisms protect against renal ischemia/reperfusion and distant organ induced injuries ScienceDirect: S1567576911001068
Atherosclerosis IL-18 overexpression promotes vascular inflammation and remodeling in a rat model of metabolic syndrome ScienceDirect: S0021915009006200
PLoS One. HIF-1α Is Essential for Effective PMN Bacterial Killing, Antimicrobial Peptide Production and Apoptosis in Pseudomonas aeruginosa Keratitis Plos: Source
临床儿科杂志 中性粒细胞明胶酶相关脂质运载蛋白、肾损伤分子1和白介素18 在诊断心肺分流术后急性肾损伤中的意义 Jcp-Sh:Source
Cell Death Dis. MicroRNA-30d regulates cardiomyocyte pyroptosis by directly targeting foxo3a in diabetic cardiomyopathy Pubmed:Pmc4237254
PLoS One. Cilostazol Renoprotective Effect: Modulation of PPAR-γ, NGAL, KIM-1 and IL-18 Underlies Its Novel Effect in a Model of Ischemia-Reperfusion Pubmed:24816434
J Korean Med Sci. Acute Kidney Injury after Using Contrast during Cardiac Catheterization in Children with Heart Disease Pubmed:25120320
PLOS ONE Protective Effect of Leaf Essential Oil from PubMed: 25794175
Fertil Steril Inflammosome in the human endometrium: further step in the evaluation of the “maternal side” PubMed: 26474737
United States Patent Application GLUCAN-ENCAPSULATED SIRNA FOR TREATING TYPE 2 DIABETES MELLITUS y2016:0138028.html
Front Pharmacol Poria Attenuates Idiosyncratic Liver Injury Induced by Polygoni Multiflori Radix Praeparata pubmed:27803670
Pharmacological reports The Role of PON-1, GR, IL-18, and oxLDL in Depression With and Without Posttraumatic Stress Disorder science:article
International Journal of Molecular Sciences PEDF Inhibits the Activation of NLRP3 Inflammasome in Hypoxia Cardiomyocytes through PEDF Receptor/Phospholipase A2 pubmed:27973457
Cellular Physiology and Biochemistry MiR-155 is Involved in Renal Ischemia-Reperfusion Injury via Direct Targeting of FoxO3a andRegulating Renal Tubular Cell Pyroptosis. pubmed:28006785
Journal of Food and Drug Analysis Anti-inflammatory effect of cinnamaldehyde and linalool from the leaf essential oil of Cinnamomum osmophloeum Kanehira in endotoxin-induced … S1021-9498(17)30081-9
Journal of Microbiology, Immunology and Infection Identification of a panel of serum protein markers in early stage of sepsis and its validation in a cohort of patients pubmed:28655573
Discovery and Validation of Immunological Biomarkers in Milk for Health Monitoring of Dairy Cows - Results from a Multiomics Approach doi:10.4172/2329-888X.1000182
Cellular Physiology and Biochemistry Dexmedetomidine Alleviates HyperoxiaInduced Acute Lung Injury via Inhibiting NLRP3 Inflammasome Activation pubmed:28873369
20 Melatonin prevents endothelial cell pyroptosis via regulation of long noncoding RNA MEG3/miR-223/NLRP3 axis pubmed:29024030
Pharmacological Reports The role of PON-1, GR, IL-18, and OxLDL in depression with and without posttraumatic stress disorder. pubmed:28623707
World journal of gastroenterology Hypothermic machine perfusion with metformin-University of Wisconsin solution for ex vivo preservation of standard and marginal liver grafts in a rat model. pubmed:29142469
Veterinary microbiology Secondary Haemophilus parasuis infection enhances highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) infection-mediated inflammatory responses. pubmed:28532803
International immunopharmacology Chronic dexamethasone treatment results in hippocampal neurons injury due to activate NLRP1 inflammasome in vitro pubmed:28605710
Journal of Neuroinflammation Chronic glucocorticoid exposure activates BK-NLRP1 signal involving in hippocampal neuron damage. pubmed:28732502
Journal of Neuroinflammation Unconjugated bilirubin induces pyroptosis in cultured rat cortical astrocytes Pubmed:29357878
Oxidative Medicine and Cellular Longevity The Effects of Sinapic Acid on the Development of Metabolic Disorders Induced by Estrogen Deficiency in Rats Pubmed:29967666
Journal of Translational Medicine Recurrent pregnancy loss is associated to leaky gut: a novel pathogenic model of endometrium inflammation? Pubmed:29665864
Chemico-Biological Interactions Nifuroxazide, a STAT3 inhibitor, mitigates inflammatory burden and protects against diabetes-induced nephropathy in rats Pubmed:29291386
Journal of Food and Drug Analysis Anti-inflammatory effect of cinnamaldehyde and linalool from the leaf essential oil of Cinnamomum osmophloeum Kanehira in endotoxin-induced mice Pubmed:29389558
International Journal of Molecular Medicine MCMV triggers ROS/NLRP3-associated inflammasome activation in the inner ear of mice and cultured spiral ganglion neurons, contributing to sensorineural hearing … Pubmed:29512778
Archives of Virology Genetic and immunogenicity analysis of porcine circovirus type 2 strains isolated in central China Pubmed:29305646
Journal of Inflammation Expression and clinical significance of the NEK7-NLRP3 inflammasome signaling pathway in patients with systemic lupus erythematosus Pubmed: 30202244
Journal of Affective Disorders Post-stroke depression and cognitive impairment: study design and preliminary findings in a Brazilian prospective stroke cohort (EMMA study) Doi: 10.1016/j.jad.2018.10.003
BioScientia Medicina Sensitivity and Spesificity of Interleukin 18 as Biomarker for Acute Kidney Injury
International Journal of Molecular Medicine Ginsenoside Rg1 protects against H2O2‑induced neuronal damage due to inhibition of the NLRP1 inflammasome signalling pathway in hippocampal neurons in vitro Pubmed: 30535505
Nutrients Effect of Rosmarinic Acid on the Serum Parameters of Glucose and Lipid Metabolism and Oxidative Stress in Estrogen-Deficient Rats Pubmed: 30691017
International immunopharmacology NADPH oxidase 2-mediated NLRP1 inflammasome activation involves in neuronal senescence in hippocampal neurons in vitro Pubmed: 30677569
Acta Histochemica Pre-administration of luteoline attenuates neonatal sevoflurane-induced neurotoxicity in mice Doi: 10.1016/j.acthis.2019.04.004
JOURNAL OF DRUG TARGETING Artemisinin alleviates atherosclerotic lesion by reducing macrophage inflammation via regulation of AMPK/NF-κB/NLRP3 inflammasomes pathway Pubmed: 31094238
IUBMB Life Melatonin Enhances Autophagy and Decreases Apoptosis Induced by nanosilica in RAW264. 7 cells Pubmed: 31018046
American Journal of Reproductive Immunology Effect of alpha lipoic acid and myoinositol on endometrial inflammasome from recurrent pregnancy loss women Pubmed: 31148259
INFLAMMATION RESEARCH TNF-α/calreticulin dual signaling induced NLRP3 inflammasome activation associated with HuR nucleocytoplasmic shuttling in rheumatoid arthritis Pubmed: 31119302
PLoS One Sepsis causes right ventricular myocardial inflammation independent of pulmonary hypertension in a porcine sepsis model Pubmed: 31247004
Journal of Dairy Science Hepatic nuclear factor kappa B signaling pathway and NLR family pyrin domain containing 3 inflammasome is over-activated in ketotic dairy cows Pubmed: 31495623
Rheumatology international Serum levels of selected cytokines [interleukin (IL)-17A, IL-18, IL-23] and chemokines (RANTES, IP10) in the acute phase of immunoglobulin A vasculitis in … Pubmed: 31468124
LIFE SCIENCES Protective effects of epigallocatechin gallate against ischemia reperfusion injury in rat skeletal muscle via activating Nrf2/HO-1 signaling pathway Pubmed: 31678278
life sciences Melatonin biosynthesis restored by CpG oligodeoxynucleotides attenuates allergic airway inflammation via regulating NLRP3 inflammasome Pubmed: 31738882
International Immunopharmacology Dexamethasone alleviate allergic airway inflammation in mice by inhibiting the activation of NLRP3 inflammasome Pubmed: 31780368
Journal of Food Science Effects of Total Flavonoids from Exocarpium Citri Grandis on Air Pollution Particle‐Induced Pulmonary Inflammation and Oxidative Stress in Mice Pubmed: 31762039
Antioxidants Effect of Rosmarinic Acid and Sinapic Acid on Oxidative Stress Parameters in the Cardiac Tissue and Serum of Type 2 Diabetic Female Rats Pubmed: 31771099
Frontiers in Immunology The Increase in IL-1β in the Early Stage of Heatstroke Might Be Caused by Splenic Lymphocyte Pyroptosis Induced by mtROS-Mediated Activation of the … Pubmed: 31921131
Pharmacological Reports The dual reno-and neuro-protective effects of dimethyl fumarate against uremic encephalopathy in a renal ischemia/reperfusion model Pubmed: 32141026
Experimental and Therapeutic Medicine Induction of autophagy protects human dental pulp cells from lipopolysaccharide‑induced pyroptotic cell death Pubmed: 32104285
Cancer Chemother Pharmacol Novel complementary antitumour effects of celastrol and metformin by targeting IκBκB, apoptosis and NLRP3 inflammasome activation in diethylnitrosamine-induced … Pubmed: 31989218
Frontiers in Immunology TLR2-Melatonin Feedback Loop Regulates the Activation of NLRP3 Inflammasome in Murine Allergic Airway Inflammation Pubmed: 32117301
molecular immunology STAT5a induces endotoxin tolerance by alleviating pyroptosis in kupffer cells Pubmed: 32298872
INVESTIGATIONAL NEW DRUGS Dual-function chimeric antigen receptor T cells targeting c-Met and PD-1 exhibit potent anti-tumor efficacy in solid tumors Pubmed: 32772342
Peptides Liraglutide ameliorates lipotoxicity-induced inflammation through the mTORC1 signalling pathway Pubmed: 32771373
molecules Juglone Suppresses LPS-induced Inflammatory Responses and NLRP3 Activation in Macrophages Pubmed: 32646056
molecular immunology TLR9 mediates the activation of NLRP3 inflammasome and oxidative stress in murine allergic airway inflammation Pubmed: 32623292
Inflammopharmacology Novel complementary coloprotective effects of metformin and MCC950 by modulating HSP90/NLRP3 interaction and inducing autophagy in rats Pubmed: 32594364
Life Sci Puerarin ameliorates retinal ganglion cell damage induced by retinal ischemia/reperfusion through inhibiting the activation of TLR4/NLRP3 inflammasome Pubmed: 32526286
Toxicol Appl Pharmacol Celastrol augments sensitivity of NLRP3 to CP-456773 by modulating HSP-90 and inducing autophagy in dextran sodium sulphate-induced colitis in rats Pubmed: 32470352
STEM CELL REVIEWS AND REPORTS A Novel Evidence That Mannan Binding Lectin (MBL) Pathway of Complement Cascade Activation is Involved in Homing and Engraftment of Hematopoietic Stem … Pubmed: 32406006
Biomedicine & Pharmacotherapy Cepharanthine attenuates cerebral ischemia/reperfusion injury by reducing NLRP3 inflammasome-induced inflammation and oxidative stress via inhibiting 12 … Pubmed: 32559840
Molecular Medicine Reports Effects of PYRIN-containing Apaf1-like protein 1 on isoflurane-induced postoperative cognitive dysfunction in aged rats Pubmed: 32626997
Food and Agricultural Immunology Acrylamide induced the activation of NLRP3 inflammasome via ROS-MAPKs pathways in Kupffer cells
FASEB JOURNAL Lactobacillus johnsonii L531 ameliorates enteritis via elimination of damaged mitochondria and suppression of SQSTM1‐dependent mitophagy in a Salmonella …
Iranian Journal of Allergy, Asthma and Immunology Increased Level of Caspase-1 in the Serum of Relapsing-remitting Multiple Sclerosis (RRMS) Patients
Biomarkers of Allergic Asthma and their Association with Serum Parameters
Drug Dev Res Paeoniflorin inhibited nod©\like receptor protein©\3 inflammasome and NF©\¦ÊB©\mediated inflammatory reactions in diabetic foot ulcer by inhibiting the chemokine?¡­ 33236457
Molecules Alpha-Lipoic Acid Plays a Role in Endometriosis: New Evidence on Inflammasome-Mediated Interleukin Production, Cellular Adhesion and Invasion 33430011
Mol Cell Biochem Intermittent high glucose induces pyroptosis of rat H9C2 cardiomyocytes via sodium¨Cglucose cotransporter 1 33608832
Life Sci Inhibition of SGLT1 protects against glycemic variability-induced cardiac damage and pyroptosis of cardiomyocytes in diabetic mice 33508297
Tissue Cell Protective effects of Oridonin against cerebral ischemia/reperfusion injury by inhibiting the NLRP3 inflammasome activation 33676236
Inflammopharmacology Dapagliflozin, an SGLT2 inhibitor, ameliorates acetic acid-induced colitis in rats by targeting NF¦ÊB/AMPK/NLRP3 axis 34002329
Arch Biochem Biophys Vitamin D ameliorates high-fat-diet-induced hepatic injury via inhibiting pyroptosis and alters gut microbiota in rats 33965368
Placenta Vitamin D alleviates hypoxia/reoxygenation-induced injury of human trophoblast HTR-8 cells by activating autophagy 34126416
Mol Med Rep ¦Ê?opioid receptor agonist, U50488H, inhibits pyroptosis through NLRP3 via the Ca2+/CaMKII/CREB signaling pathway and improves synaptic plasticity in ¡­ 34036389
J Mol Cell Cardiol CircHelz activates NLRP3 inflammasome to promote myocardial injury by sponging miR-133a-3p in mouse ischemic heart 34043986
Med Sci Monit Post-Treatment Sevoflurane Protects Against Hypoxic-Ischemic Brain Injury in Neonatal Rats by Downregulating Histone Methyltransferase G9a and Upregulating ¡­ 34059615
Biological Chemistry HDAC8 promotes daunorubicin resistance of human acute myeloid leukemia cells via regulation of IL-6 and IL-8 33938176
BioMed Research International Ultrashort Wave Combined with Human Umbilical Cord Mesenchymal Stem Cell (HUC-MSC) Transplantation Inhibits NLRP3 Inflammasome and Improves?¡­ 33376718
Evidence-Based Complementary and Alternative Medicine Zisheng Shenqi Decoction Ameliorates Monosodium Urate-Mediated Gouty Arthritis in Rats via Promotion of Autophagy through the AMPK/mTOR Signaling?¡­ 33505502
BioMed Research International MiR-599 Protects Cardiomyocytes against Oxidative Stress-Induced Pyroptosis 33681353
Annals of Tropical Medicine & Public Health Effectiveness of biomarkers and serum parameters in determination allergic asthma and detection of its severity
Mediators of Inflammation Sparstolonin B Exerts Therapeutic Effects on Collagen-Induced Arthritis by Inhibiting the NLRP3 Inflammasome and Reducing the Activity of ¦Á1, 3 ¡­
J Neuroinflammation Inhibition of heat shock protein family A member 8 attenuates spinal cord ischemia–reperfusion injury via astrocyte NF-κB/NLRP3 inflammasome pathway 34362408
Mol Med Rep Sacubitril/valsartan (LCZ696) reduces myocardial injury following myocardial infarction by inhibiting NLRP3‑induced pyroptosis via the TAK1/JNK signaling pathway 34296299
Int Immunopharmacol The protective effect of isoflurane pretreatment on liver IRI by suppressing noncanonical pyroptosis of liver macrophages 34332342
LIFE SCIENCES The NLRP3 inhibitor dapansutrile attenuates folic acid induced nephrotoxicity via inhibiting inflammasome/caspase-1/IL axis and regulating autophagy/proliferation 34560082
Biochim Biophys Acta Mol Cell Biol Lipids Elaidic acid induced NLRP3 inflammasome activation via ERS-MAPK signaling pathways in Kupffer cells 34610469
Inflammation Emodin Attenuates LPS-Induced Acute Lung Injury by Inhibiting NLRP3 Inflammasome-Dependent Pyroptosis Signaling Pathway In vitro and In vivo 34787801
J Agric Food Chem Allicin Ameliorates Intestinal Barrier Damage via Microbiota-Regulated Short-Chain Fatty Acids-TLR4/MyD88/NF-κB Cascade Response in Acrylamide-Induced Rats 34694121
Journal of Inflammation Research The Supportive Role of NSC328382, a P2X7R Antagonist, in Enhancing the Inhibitory Effect of CRID3 on NLRP3 Inflammasome Activation in Rats with Dextran … 34321905
Viruses Interleukin-10-Mediated Lymphopenia Caused by Acute Infection with Foot-and-Mouth Disease Virus in Mice 34960627
Toxicology Salidroside alleviates liver inflammation in furan-induced mice by regulating oxidative stress and endoplasmic reticulum stress 34450210
Antioxidants Key Mechanisms and Potential Implications of Hericium erinaceus in NLRP3 Inflammasome Activation by Reactive Oxygen Species during Alzheimer's Disease 34829535
Ecotoxicol Environ Saf Silica nanoparticles induce pulmonary autophagy dysfunction and epithelial-to-mesenchymal transition via p62/NF-κB signaling pathway Pubmed:35158278
J Dairy Sci Overactivation of hepatic mechanistic target of rapamycin kinase complex 1 (mTORC1) is associated with low transcriptional activity of transcription factor EB … Pubmed:35248377
Free Radic Biol Med Silica nanoparticles induce pyroptosis and cardiac hypertrophy via ROS/NLRP3/Caspase-1 pathway Pubmed:35219847
International Heart Journal Melatonin Inhibits OGD/R-Induced H9c2 Cardiomyocyte Pyroptosis via Regulation of MT2/miR-155/FOXO3a/ARC Axis Pubmed:35354753
Journal of Integrative Neuroscience Gastrodin suppresses pyroptosis and exerts neuroprotective effect in traumatic brain injury model by inhibiting NLRP3 inflammasome signaling pathway Pubmed:35364660
Perfusion Insulin reduces pyroptosis-induced inflammation by PDHA1 dephosphorylation-mediated NLRP3 activation during myocardial ischemia-reperfusion injury Pubmed:35506656
Ecotoxicol Environ Saf Ambient ozone exposure induces ROS related-mitophagy and pyroptosis via NLRP3 inflammasome activation in rat lung cells Pubmed:35642860
Medicina Plasma Concentrations of Cytokines in Patients with Combined Hyperlipidemia and Atherosclerotic Plaque before Treatment Initiation—A Pilot Study Pubmed:35630041
Pyroptosis Accelerates Spiral Ganglion Neuronal Degeneration Induced by Aminoglycosides in the Cochlea
researchsquare Dexmedetomidine Attenuates Hyperalgesia Induced By Brachial Plexus Root Avulsion By Restoring The GLT-1 Function Via PKA Signaling
Reseach square Assessment of selected interleukins (IL-6, IL-17A, IL-18, IL-23) and chemokines (RANTES, IP-10) in children with acute and chronic urticaria
Anti-inflammatory activity of non-selective PDE inhibitor aminophylline on the lung tissue and respiratory parameters in animal model of ARDS
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPA064Mu02 Recombinant Interleukin 18 (IL18) Positive Control; Immunogen; SDS-PAGE; WB.
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