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Active Interleukin 15 (IL15)

ACTIVITY TEST

Interleukin 15 (IL15) is a widely expressed cytokine that is structurally and functionally related to IL2, which plays an important role in many immunological diseases. IL15 also regulates T and natural killer (NK) cell activation and proliferation. The activity of recombinant human IL15 was measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. MO7e cells were seeded into triplicate wells of 96-well plates at a density of 30,000 cells/well in RPMI-1640 with the addition of various concentrations of rhIL15. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 µl of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 °C. Cell proliferation of MO7e cells after incubation with rhIL15 for 72h observed by inverted microscope was shown in Figure 1. The dose-effect curve of rhIL15 was shown in Figure 2. It was obvious that rhIL15 significantly promoted cell proliferation of MO7e cells .The EC50 for this effect is typically 0.094 ug/ml.

Figure 2. The dose-effect curve of rhIL15 on MO7e cells

USAGE

Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Endocrine Expression of interleukin-15 and inflammatory cytokines in skeletal muscles of STZ-induced diabetic rats: effect of resistance exercise training Pubmed: 24006180
Endocrine Time course of IL-15 expression after acute resistance exercise in trained rats: effect of diabetes and skeletal muscle phenotype Pubmed:25522723
Med Sci Moni miR-15b is Downregulated in Myasthenia Gravis Patients and Directly Regulates the Expression of Interleukin-15 (IL-15) in Experimental Myasthenia Gravis Mice PubMed: 26087886
Exercise physiology Effects of intrinsic aerobic capacity, aging and physical activity on interleukin-15 protein level in serum and skeletal muscle Dspace: Handle
Biotechnology Letters Novel adjuvant for immunization against tuberculosis: DNA vaccine expressing Mycobacterium tuberculosis antigen 85A and interleukin-15 fusion product elicits strong immune responses in mice s10529-017-2342-1
Journal of Controlled Release Interleukin-15 and cisplatin co-encapsulated thermosensitive polypeptide hydrogels for combined immuno-chemotherapy. pubmed:28408199
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
APA061Hu01 Active Interleukin 15 (IL15) Cell culture; Activity Assays.
RPA061Hu01 Recombinant Interleukin 15 (IL15) Positive Control; Immunogen; SDS-PAGE; WB.
PAA061Hu01 Polyclonal Antibody to Interleukin 15 (IL15) WB; IHC; ICC; IP.
LAA061Hu71 Biotin-Linked Polyclonal Antibody to Interleukin 15 (IL15) WB; IHC; ICC.
RAA061Hu21 Recombinant Antibody to Interleukin 15 (IL15) WB; IF; ICC; IHC; IP; FCM.
MAA061Hu21 Monoclonal Antibody to Interleukin 15 (IL15) WB
MAA061Hu22 Monoclonal Antibody to Interleukin 15 (IL15) WB; IHC; ICC; IP.
SEA061Hu ELISA Kit for Interleukin 15 (IL15) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA061Hu Multiplex Assay Kit for Interleukin 15 (IL15) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
IGA061Hu21 Mouse Knockin Human Interleukin 15 (IL15) Animal Experiments; Positive Control.