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Active Insulin Like Growth Factor Binding Protein 3 (IGFBP3)

BP53; IBP3; Growth Hormone-Dependent Binding Protein; Acid Stable Subunit Of The 140 K IGF Complex

ACTIVITY TEST

Insulin-like growth factor-binding protein 3, also known as IGFBP3 is one of six
IGF binding proteins (IGFBP1 to IGFBP6) that have highly conserved structures
and bind the insulin-like growth factors IGF-1 and IGF-2 with high affinity. Within
tissues, IGFBP3 can bind IGF1 and IGF2 released by many cell types, and block
their access to the IGF-1 receptor (IGF1R), which is activated by both IGFs.
IGFBP3 also interacts with cell-surface proteins, affecting cell signaling from
outside the cell or after internalization, and also enters the cell nucleus where it
binds to nuclear hormone receptors and other ligands. Besides, Fibronectin (FN)
has been identified as an interactor of IGFBP3, thus a binding ELISA assay was
conducted to detect the interaction of recombinant human IGFBP3 and
recombinant human FN.
Briefly, IGFBP3 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate
samples of 100ul were then transferred to FN-coated microtiter wells and
incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h
with anti-IGFBP3 pAb, then aspirated and washed 3 times. After incubation with
HRP labelled secondary antibody, wells were aspirated and washed 5 times.
With the addition of substrate solution , wells were incubated 15-25 minutes at
37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately.
The binding activity of IGFBP3 and FN was shown in Figure 1, the EC50 was 78
ng/ml.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Molecular Biology Reports Characterization of the specific and sustained GH1 expression induced by rAAV2/1 in normal adult male rats PubMed: 20204528
Endocrinology Role of IGFBP-3 in the Regulation of β-Cell Mass during Obesity: Adipose Tissue/β-Cell Cross Talk Endo: source
Nutrition and Cancer A Pilot Study Comparing the Effect of Flaxseed, Aromatase Inhibitor, and the Combination on Breast Tumor Biomarkers Tandfonline: Source
Dig Dis Sci The Protective Effects of Exclusive Enteral Nutrition Formulas on Growth Factor Expression and the Proximal Tibial Epiphyseal Growth Plate in a TNBS-Induced IBD Rat Model PubMed: 25708898
Animal. Effects of a dietary crude fibre concentrate on growth in weaned piglets. pubmed:28318464
PLoS One Altered insulin-like growth factor-2 signaling is associated with psychopathology and cognitive deficits in patients with schizophrenia Pubmed: 32191705
Int J Environ Res Public Health Association of Salivary IGF and IGF/IGFBP-3 Molar Ratio with Cervical Vertebral Maturation Stages from Pre-Adolescent to Post-Adolescent Transition Period—A … Pubmed:35564566
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