Active Insulin Like Growth Factor 1 (IGF1)
IGFI; IGF1A; IBP1; MGF; Somatomedin C; Mechano Growth Gactor
- Product No.APA050Mu02
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- Traits Freeze-dried powder
- Purity> 97%
- Isoelectric Point9.7
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
- FOB
US$ 122
For more details, please contact local distributors! US$ 304 US$ 608 US$ 1824 US$ 4560
ACTIVITY TEST
![](/upload/product/pictures/APA050Mu02-202305091438567290.jpg)
Insulin-like growth factor 1 (IGF1), also called somatomedin C is a hormone similar in molecular structure to insulin. It plays an important role in childhood growth and continues to have anabolic effects in adults. A synthetic analog of IGF1, mecasermin, is used for the treatment of growth failure. Besides, Insulin Like Growth Factor Binding Protein 5 (IGFBP5) has been identified as an interactor of IGF1, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse IGF1 and recombinant mouse IGFBP5. Briefly, IGF1 were diluted serially in PBS, with 0.01% BSA (pH7.4). Duplicate samples of 100 μl were then transferred to IGFBP5-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-IGF1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450 nm immediately. The binding activity of IGF1 and IGFBP5 was shown in Figure 1, and this effect was in a dose dependent manner, the EC50 was 2.92 ug/ml.
![](/upload/product/pictures/APA050Mu02-202305091439589708.jpg)
To measure the effect of IGF1 on cell proliferation, breast cancer MCF-7 cells were seeded into triplicate wells of 96-well plates at a density of 5,000 cells/well and allowed to attach, replaced with serum-free overnight, then the medium was replaced with 1% serum standard DMEM prior to the addition of various concentrations of recombinant mouse IGF1. After incubated for 96h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µl of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37℃. Proliferation of MCF-7 cells after incubation with IGF1 for 96h observed by inverted microscope was shown in Figure1. Cell viability was assessed by CCK-8 assay after incubation with recombinant IGF1 for 96h. The result was shown in Figure2. It was obvious that IGF1 significantly increased cell viability of MCF-7 cells.
![](/upload/product/pictures/APA050Mu02-202305091440206228.jpg)
Figure 2. Cell proliferation of MCF-7 cells after stimulated with IGF1.
USAGE
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
-
BCA Protein Quantification Kit
-
Molecular Mass Marker for Protein
-
Monoclonal Antibody Customized Service
-
Polyclonal Antibody Customized Service
-
Protein Activity Test Experiment Service
-
Electrophoretic Mobility Shift Assay (EMSA) Experiment Service
-
Buffer
-
Lentivirus Packaging Experiment Service
-
Adenovirus Packaging Experiment Service
-
Real Time PCR Experimental Service
-
Spike RBD Protein (S-RBD)
-
Protein G
-
Protein A
Magazine | Citations |
Cellular & Molecular Biology Letters | A microarray gene analysis of peripheral whole blood in normal adult male rats after long-term GH gene therapy PubMed: 20119855 |
Molecular Biology Reports | Characterization of the specific and sustained GH1 expression induced by rAAV2/1 in normal adult male rats PubMed: 20204528 |
Regulatory Peptides | The vagus nerve as a regulator of growth hormone secretion PubMed: 21056594 |
Vascular Cell | Insulin-Like Growth Factor-1 (IGF-1) Reduces Ischemic Changes and Increases Circulating Angiogenic Factors in Experimentally - Induced Myocardial Infarction in Rats PubMed: 21651821 |
27 | Physical activity attenuates intermittent hypoxia-induced spatial learning deficits and oxidative stress. PubMed: 20224062 |
Journal of Pediatric Surgery | The influence of nutrients, biliary-pancreatic secretions, and systemic trophic hormones on intestinal adaptation in a Roux-en-Y bypass model PubMed: 20438940 |
The Veterinary Journal | Serum insulin-like growth factor type 1 concentrations in healthy dogs and dogs with spontaneous primary hypothyroidism PubMed: 21546289 |
Developmental Biology | Effects of monochromatic light on developmental changes in satellite cell population of pectoral muscle in broilers during early posthatch period Wiley: source |
Journal of Neuroendocrinology | Interleukin-6 Regulates the Expression of Hypothalamic Neuropeptides Involved in Body Weight in a Gender-Dependent Way Wiley: source |
International Journal of Molecular Sciences | Insulin Like Growth Factor-1 (IGF-1) Causes Overproduction of IL-8, an Angiogenic Cytokine and Stimulates Neovascularization in Isoproterenol-Induced Myocardial Infarction in Rats Mdpi: 8562 |
Pharmacology | Protective Effect of Amlodipine on Rat Bone Tissue after Orchidectomy Karger: 335491 |
Bulletin of the Veterinary Institute in Pulawy | Maternal Glucocorticoid Treatment as a Model for Examining Foetal Gender-Specific Effects on the Bone Metabolism of Neonatal Piglets Degruyter: Source |
BMC cell biology | BMSCs reduce rat granulosa cell apoptosis induced by cisplatin and perimenopause PubMed: PMC3640998 |
Journal of Veterinary Science | Evaluation of IGF-I levels and serum protein profiles of diabetic cats and dogs PubMed: PMC3232391 |
PLoS ONE | A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches Plosone: Source |
Pesquisa Veterinária Brasileira | Porcine follicular fluid concentration of free insulin-like growth factor-I collected from different diameter ovarian follicles Scielo: Source |
international Journal of food and nutritional sciences | EVALUATION OF PHYSICO-CHEMICAL PROPERTIES OF COLOSTRUM SUPPLEMENTED DAHI Ijfans: Source |
Theriogenology | Role of insulin-like growth factor-I and follicular fluid from ovarian follicles with different diameters on porcine oocyte maturation and fertilization in vitro ScienceDirect: S0093691X13001659 |
Biosensors and Bioelectronics | Electrochemical immunosensor for the determination of insulin-like growth factor-1 using electrodes modified with carbon nanotubes–poly(pyrrole propionic acid) hybrids ScienceDirect: S0956566313005629 |
Electromagnetic Biology and Medicine | The effects of long-term exposure to a 2450 MHz electromagnetic field on growth and pubertal development in female Wistar rats Informahealthcare: Source |
Nutrition and Cancer | A Pilot Study Comparing the Effect of Flaxseed, Aromatase Inhibitor, and the Combination on Breast Tumor Biomarkers Tandfonline: Source |
Bases de Dados da Pesquisa Agropecuária | CONCENTRACOES SéRICAS DE INSULINA NO PóS-PARTO RECENTE DE VACAS DE CORTE DE DIFERENTES GENóTIPOS CRIADAS NO SUL DO BRASIL Embrapa: Source |
International Journal of Endocrinology | Negative Energy Balance Induced by Paradoxical Sleep Deprivation Causes Multicompartmental Changes in Adipose Tissue and Skeletal Muscle Cepebr:Source |
J Neurol Sci. | The effect of topiramate and lamotrigine on rat bone mass, structure and metabolism. Pubmed:24629477 |
Reproductive Sciences | Effects of Resveratrol on Ovarian Morphology, Plasma Anti-Mullerian Hormone, IGF-1 Levels, and Oxidative Stress Parameters in a Rat Model of Polycystic Ovary Syndrome Pubmed:25667201 |
Int J Clin Exp Med | Effects of sericin on the testicular growth hormone/insulin-like growth factor-1 axis in a rat model of type 2 diabetes PubMed: 26379831 |
Int J Clin Exp Med. | The effects of self-assembling peptide RADA16 hydrogel on malignant phenotype of human hepatocellular carcinoma cell PubMed: 26628972 |
Animal and Biomedical Sciences | The use of herb mix and lucerne to increase growth rates of dairy heifers System: Files |
Neonatal Biology | Maternal Nutrition with Β-Hydroxy-Β-Methylbutyrate as Strong Determinants of the Development of Newborn Offspring in Pigs Journals: 1000183 |
Dig Dis Sci | The Protective Effects of Exclusive Enteral Nutrition Formulas on Growth Factor Expression and the Proximal Tibial Epiphyseal Growth Plate in a TNBS-Induced IBD Rat Model PubMed: 25708898 |
Tumor Biology | Hypoxia-induced secretion of IL-12 from adipose-derived mesenchymal stem cell promotes growth and cancer stem cell properties of Burkitt lymphoma PubMed: 26695151 |
Forage Res | RELATIONSHIP OF RESIDUAL FEED INTAKE WITH BLOOD METABOLITES AND HORMONES IN SAHIWAL FEMALE CALVES Wp-Content: Uploads |
S | Caracteriza Teses: Disponiveis |
Photochemistry AND Photobiology | Melatonin Mediates Monochromatic Light‐induced Insulin‐like Growth Factor 1 Secretion of Chick Liver: Involvement of Membrane Receptors Pubmed:27128575 |
Pediatric Pulmonology | Protective effect of vitamin D against hyperoxia‐induced lung injury in newborn rats Pubmed:27291304 |
Hippocampus | A single bout of resistance exercise improves memory consolidation and increases the expression of synaptic proteins in the hippocampus Pubmed:27008926 |
Neurotoxicity Research | Cigarette Smoke Delays Regeneration of the Olfactory Epithelium in Mice Pubmed:27003941 |
ANNALS OF ANIMAL SCIENCE | Comparison of the effect of a standard inclusion level of inorganic zinc to organic form at lowered level on bone development in growing male Ross broiler chickens journalprintahead:87 |
Annals of Animal Science | Comparison of the Effect of a Standard Inclusion Level of Inorganic Zinc to Organic Form at Lowered Level on Bone Development in Growing Male Ross Broiler Chickens view:87 |
African Health Sciences | Effects of acute exercise on salivary free insulin-like growth factor 1 and interleukin 10 in sportsmen. pubmed:27605972 |
PLoS One. | Paradoxical Sleep Deprivation Causes Cardiac Dysfunction and the Impairment Is Attenuated by Resistance Training. pubmed:27880816 |
international journal of molecular sciences | Expression of Iron-Related Proteins Differentiate Non-Cancerous and Cancerous Breast Tumors. pubmed:28216608 |
Biochemistry and Cell Biology | Chronic intermittent hypoxia disturbs insulin secretion and causes pancreatic injury via theMAPK signaling pathway. pubmed:28177762 |
Neurochemical Research | Resistance Exercise Reduces Seizure Occurrence, Attenuates Memory Deficits and Restores BDNF Signaling in Rats with Chronic Epilepsy article:10.1007 |
Journal of Agricultural Biotechnology | GH蛋白诱导鸡原代成肌细胞表达IGF1条件优化 10697 |
Trop Anim Health Prod | Assessment of feeding varying levels of Metabolizable energy and protein on performance of transition Murrah buffaloes pubmed:28785907 |
Molecular Medicine Reports | Mesenchymal stem cells promote endothelial progenitor cell proliferation by secreting insulin‑like growth factor‑1 pubmed:28627605 |
Neurochem Res | Resistance Exercise Reduces Seizure Occurrence, Attenuates Memory Deficits and Restores BDNF Signaling in Rats with Chronic Epilepsy pubmed:28078614 |
Biological Trace Element Research | Effect of Dietary Phytase Supplementation on Bone and Hyaline Cartilage Development of Broilers Fed with Organically Complexed Copper in a Cu-Deficient Diet article:10.1007 |
Revista Brasileira de Ciência Avícola | Effect of Zinc Level and Source (Zinc Oxide Vs. Zinc Glycine) on Bone Mechanical and Geometric Parameters, and Histomorphology in Male Ross 308 Broiler Chicken 10.159:1806-9061-2016-0285 |
International Journal of Chemical Studies | Studies on quality attributes of skimmed colostrum powder P-ISSN: 2349–8528 |
Stem Cell Research & Therapy | Effects of low-intensity pulsed ultrasound (LIPUS)-pretreated human amnion-derived mesenchymal stem cell (hAD-MSC) transplantation on primary ovarian insufficiency in rats 10.1186/s13287-017-0739-3 |
PLOS one | A metabolite of leucine (β-hydroxy-β-methylbutyrate) given to sows during pregnancy alters bone development of their newborn offspring by hormonal modulation 10.1371/journal.pone.0179693 |
Journal of Controlled Release | A DPP-IV-resistant glucagon-like peptide-2 dimer with enhanced activity against radiation-induced intestinal injury pubmed:28522195 |
Obesity Surgery | Effects of Bariatric Surgery on Change of Brown Adipocyte Tissue and Energy Metabolism in Obese Mice pubmed:28853013 |
Journal of Nutritional Sciences | Effect of dietary protein intake on the body composition and metabolic parameters of neutered dogs pubmed:29152244 |
Inflammation | Involvements of γδT Lymphocytes in Acute and Chronic Skin Wound Repair. pubmed:28540539 |
Frontiers in Molecular Neuroscience | Rosiglitazone Exerts an Anti-depressive Effect in Unpredictable Chronic Mild-Stress-Induced Depressive Mice by Maintaining Essential Neuron Autophagy and Inhibiting Excessive Astrocytic Apoptosis. pubmed:28959186 |
Journal of Photochemistry and Photobiology | Various LED Wavelengths Affected Myofiber Development and Satellite Cell Proliferation of Chick Embryos via the IGF-1 Signaling Pathway. pubmed:28708285 |
Brain, Behavior, and Immunity | An enriched environment restores hepatitis B vaccination-mediated impairments in synaptic function through IFN-γ/Arginase1 signaling Pubmed:29627530 |
Journal of Translational Medicine | Generation of GHR-modified pigs as Laron syndrome models via a dual-sgRNAs/Cas9 system and somatic cell nuclear transfer Pubmed:29482569 |
PLoS One | Subsequent somatic axis and bone tissue metabolism responses to a low-zinc diet with or without phytase inclusion in broiler chickens Pubmed:29373588 |
Research in Veterinary Science | Evaluation of serum insulin-like growth factor-1 and 26S proteasome concentrations in healthy dogs and dogs with chronic diseases depending on body condition … 10.1016:j.rvsc.2018.04.009 |
Pharmacology | Effects of Amlodipine on Bone Metabolism in Orchidectomised Spontaneously Hypertensive Rats Pubmed:29898457 |
Tropical Animal Health and Production | Dietary supplementation for Santa Inês hair ewes on pasture at pre-and postpartum periods: dry matter intake, digestibility, milk production, and mineral metabolism Pubmed:29931604 |
CANADIAN JOURNAL OF VETERINARY RESEARCH-REVUE CANADIENNE DE RECHERCHE VETERINAIRE | 26S proteasome and insulin-like growth factor-1 in serum of dogs suffering from malignant tumors Pubmed:29755191 |
Pakistan Journal of Zoology | Effects of Intrauterine Growth Retardation on Growth, Meat Quality and Muscle Fiber Composition of Pigs. : |
MEDYCYNA WETERYNARYJNA | Circulating concentrations of insulin like growth factor-1 in female dogs with spontaneous mammary tumours 10.21521:mw.6054 |
Oncotarget | Nucleoside reverse transcriptase inhibitor-induced rat oocyte dysfunction and low fertility mediated by autophagy Pubmed:29423092 |
Science Citation Index | Effect of porcine somatotropin on metabolism, testicular size and sperm characteristics in young boars 10.1590:1678-4162-9481 |
Journal of Animal Science | Fermented corn-soybean meal elevated IGF1 levels in grower-finisher pigs Pubmed: 30203098 |
Journal of Animal Physiology and Animal Nutrition | Maternal HMB treatment affects bone and hyaline cartilage development in their weaned piglets via the leptin/osteoprotegerin system Pubmed: 30659706 |
Biomedicine & Pharmacotherapy | The therapeutic effects of nicotinamide in hepatocellular carcinoma through blocking IGF-1 and effecting the balance between Nrf2 and PKB Pubmed: 30784932 |
The American Journal of Tropical Medicine and Hygiene | Association between Insulin-Like Growth Factor-I Levels and the Disease Progression and Anemia in Visceral Leishmaniasis Pubmed: 30761980 |
PLoS One | Light-dark rhythms during incubation of broiler chicken embryos and their effects on embryonic and post hatch leg bone development Pubmed: 30682088 |
International Journal of Molecular Sciences | Mel1c Mediated Monochromatic Light-Stimulated IGF-I Synthesis through the Intracellular Gαq/PKC/ERK Signaling Pathway Pubmed: 30987295 |
Animal Reproduction Science | Serum and seminal plasma IGF-1 associations with semen variables and effect of IGF-1 supplementation on semen freezing capacity in buffalo bulls Pubmed: 30914211 |
PLoS One | Melatonin mediates monochromatic green light-induced satellite cell proliferation and muscle growth in chick embryo Pubmed: 31059537 |
Journal of Innovations in Pharmaceutical and Biological Sciences | Preventive effect of dried plum extract against dexamethasone-induced osteoporosis in male rats through inhibiting cathepsin-K activity, lipogenesis and … |
Buffalo Bulletin | Alterations in haemato-biochemical profile following by-pass nutrients supplementation in early lactating Murrah buffaloes |
Theriogenology | Effect of betaine supplementation on growth performance, nutrient intake and expression of IGF-1 in Karan Fries heifers during thermal stress Pubmed: 31711708 |
Journal of Animal Physiology and Animal Nutrition | Effects of a diet enriched with eicosapentaenoic, docosahexaenoic and glutamine on cytokines as immunological markers for systemic inflammation in bitches before … Pubmed: 31637790 |
Psychiatry Research | The association between serum insulin-like growth factor 1 and cognitive impairments in patients with schizophrenia Pubmed: 31839419 |
International Journal of Molecular Sciences | BST Stimulation Induces Atrophy and Changes in Aerobic Energy Metabolism in Rat Skeletal Muscles—The Biphasic Action of Endogenous Glucocorticoids Pubmed: 32316389 |
Genes (Basel) | Fenugreek Stimulates the Expression of Genes Involved in Milk Synthesis and Milk Flow through Modulation of Insulin/GH/IGF-1 Axis and Oxytocin Secretion Pubmed: 33081164 |
international journal of molecular sciences | Cytokine and Growth Factor Delivery from Implanted Platelet-Rich Fibrin Enhances Rabbit Achilles Tendon Healing Pubmed: 32370144 |
Animals | Is Dietary 2-Oxoglutaric Acid Effective in Accelerating Bone Growth and Development in Experimentally-Induced Intrauterine Growth Retarded Gilts? Pubmed: 32331362 |
Indian Journal of Animal Sciences | Peripheral concentrations of metabolic and inflammatory indicators during transition period and their relationship with postpartum clinical endometritis in dairy … |
Journal of Diabetes Research | Changes and Risk Factors of Skeletal Muscle Mass and Strength in Patients with Type 2 Diabetes over 60 Years Old: A Cross-Sectional Study from China |
ranian Journal of Basic Medical Sciences | Therapeutic effects of organic zinc on reproductive hormones, insulin resistance and |
INFLUENCE OF OMEGA–3 RICH FATTY ACID ENRICHED FEED ON BIOCHEMICAL MARKERS AND POST PARTUM REPRODUCTIVE PERFORMANCE OF … | |
Stem Cell Reviews and Reports | Prospects on the Potential In Vitro Regenerative Features of Mechanically Treated-Adipose Tissue for Osteoarthritis Care 33469783 |
Aesthet Surg J | Adipose-Derived Stem Cells Improve the Aging Skin of Nude Mice by Promoting Angiogenesis and Reducing Local Tissue Water 33428732 |
Animal | Effect of alfalfa hay and starter feed supplementation on caecal microbiota and fermentation, growth, and health of yak calves 33610512 |
Animals (Basel) | Handling Associated with Drenching Does Not Impact Survival and General Health of Low Birth Weight Piglets. Animals 2021, 11, 404 33562568 |
Bioact Mater | New design to remove leukocytes from platelet-rich plasma (PRP) based on cell dimension rather than density 33842739 |
Stem Cells International | The Paracrine Effect of Adipose-Derived Stem Cells Orchestrates Competition between Different Damaged Dermal Fibroblasts to Repair UVB-Induced Skin?¡ 33381190 |
Animals | The Protective Role of Alpha-Ketoglutaric Acid on the Growth and Bone Development of Experimentally Induced Perinatal Growth-Retarded Piglets 33435211 |
The pharma innovation Journal | Comparative study on genital status, energy balance and mineral content of swamp buffalo (Luit) heifers and cows under organized system of rearing in Assam?¡ |
Br J Nutr | The Association of Pre-Pregnancy BMI On Leptin, Ghrelin, Adiponectin, and IGF-1 In Breast Milk, A Case Control Study 34348809 |
animals | Effects of Concentrate and Bypass Fat Supplementations on Growth Performance, Blood Profile, and Rearing Cost of Feedlot Buffaloes 34359233 |
Int J Environ Res Public Health | Association of Salivary IGF and IGF/IGFBP-3 Molar Ratio with Cervical Vertebral Maturation Stages from Pre-Adolescent to Post-Adolescent Transition Period—A … Pubmed:35564566 |