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Active Hepatocyte Growth Factor (HGF)

HGF; F-TCF; HGFB; HPTA; SF; Scatter Factor; Hepapoietin A; Fibroblast-Derived Tumor Cytotoxic Factor; Lung Fibroblast-Derived Mitogen

ACTIVITY TEST

Hepatocyte Growth Factor (HGF), also known as scatter factor, is a pleiotropic cytokine primarily produced by mesenchymal cells. It plays crucial roles in embryonic development, tissue regeneration, and wound healing by mediating cell proliferation, motility, and morphogenesis. HGF is synthesized as an inactive single-chain precursor (pro-HGF) that is proteolytically cleaved to form the active heterodimer. Its signaling is vital in organogenesis and contributes to pathological processes like cancer metastasis when dysregulated. HGF binds specifically to its receptor, HGFR (c-MET), triggering intracellular pathways that promote cellular responses including proliferation and migration.To detect the activity of recombinant HGF a functional ELISA assay was performed to evaluate the interaction between recombinant mouse HGF and recombinant human HGFR.Briefly, HGF was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to HGFR-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-HGF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant mouse HGF and recombinant human HGFR was shown in Figure 1, the EC50 for this effect is 0.12µg/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Gene Therapy and Molecular Biology Sphingosine kinase-1 protects transplanted mesenchymal stem cells and improves the performance of the infarcted heart LWW: 01000
Journal of Translational Medicine Low temperature of radiofrequency ablation at the target sites can facilitate rapid progression of residual hepatic VX2 carcinoma BioMed: 14795876
Digestive Diseases and Sciences Gene Transfer of c-met Confers Protection Against d-Galactosamine/Lipopolysaccharide-Induced Acute Liver Failure SpringerLink: k22823l138x2344x
Biomarker insights Correlation Between Hepatocyte Growth Factor (HGF) and Gamma-Aminobutyric Acid (GABA) Plasma Levels in Autistic Children PubMed: PMC3694825
Biomarker insights Decreased Hepatocyte Growth Factor (HGF) and Gamma Aminobutyric Acid (GABA) in Individuals with Obsessive-Compulsive Disorder (OCD) PubMed: PMC3762604
BMC cell biology BMSCs reduce rat granulosa cell apoptosis induced by cisplatin and perimenopause PubMed: PMC3640998
Cell Journal (Yakhteh) Effect of Mesenchymal Stem Cells on Doxorubicin-Induced Fibrosis PubMed: PMC3584430
Gastroenterol Res Pract Proinflammatory Cytokines (IL-1α, IL-6) and Hepatocyte Growth Factor in Patients with Alcoholic Liver Cirrhosis PubMed: 26448742
Tumor Biology Hypoxia-induced secretion of IL-11 from adipose-derived mesenchymal stem cell promotes growth and cancer stem cell properties of Burkitt lymphoma PubMed: 26695151
Cytotherapy Cell type–dependent variation in paracrine potency determines therapeutic efficacy against neonatal hyperoxic lung injury PubMed: 25863963
Biomedicine & Pharmacotherapy Silymarin-loaded Eudragit® RS100 nanoparticles improved the ability of silymarin to resolve hepatic fibrosis in bile duct ligated rats Pubmed:27261582
Can J Physiol Pharmacol.  Piceatannol increases the expression of hepatocyte growth factor and IL-10 thereby protecting hepatocytes in thioacetamide-induced liver fibrosis Pubmed:27186801
Genet Mol Res Changes in growth factor levels in the cerebrospinal fluid of autism patients after transplantation of human umbilical cord blood mononuclear cells and umbilical cord-derived mesenchymal stem cells Pubmed:27323064
Changes in HGF levels after a progressive test before and after Tabata HIIT program profile:Pawel_Wolanski3
Stem Cell Research & Therapy Effects of low-intensity pulsed ultrasound (LIPUS)-pretreated human amnion-derived mesenchymal stem cell (hAD-MSC) transplantation on primary ovarian insufficiency in rats 10.1186/s13287-017-0739-3
Oncology Letters Carcinoma associated fibroblasts derived from oral squamous cell carcinoma promote lymphangiogenesis via c‑Met/PI3K/AKT in vitro 10.3892/ol.2017.7301
Stem Cell Research & Therapy Overexpression of c-Met in bone marrow mesenchymal stem cells improves their effectiveness in homing and repair of acute liver failure. pubmed:28679425
Nature Communications Pulmonary pericytes regulate lung morphogenesis Pubmed:29934496
Experimental And Molecular Medicine Interleukin-1 receptor antagonist-mediated neuroprotection by umbilical cord-derived mesenchymal stromal cells following transplantation into a rodent stroke … Pubmed:29650950
Oncology Letters Carcinoma associated fibroblasts derived from oral squamous cell carcinoma promote lymphangiogenesis via c-Met/PI3K/AKT in vitro Pubmed:29375714
Bulletin of Experimental Biology and Medicine Dynamics of Expression of Cytokine Genes and Macrophage Content in the Lungs and Kidneys after Subtotal Hepatectomy in Rats Pubmed:29796817
Cell Biology International Inherent control of hepatocyte proliferationInherent control of hepatocyte proliferation after subtotal liver resection Pubmed: 31297922
Journal of stem cells & regenerative medicine What is the Best Degree of Hyaluronic Acid Crosslinking in Increasing Growth Factors Level of Platelet-Rich Fibrin Lysate? Pubmed: 31239605
Open Journal of Veterinary Medicine Efficacy of autologous bone marrow mononuclear cell transplantation in dogs with chronic spinal cord injury Pubmed: 32821665
Stem Cell Reviews and Reports Prospects on the Potential In Vitro Regenerative Features of Mechanically Treated-Adipose Tissue for Osteoarthritis Care 33469783
Molecular Biology Reports Umbilical cord-derived mesenchymal stem cell conditioned medium reverses neuronal oxidative injury by inhibition of TRPM2 activation and the JNK signaling … Pubmed:35585377
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
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