≡

Active Growth Hormone (GH)

GH1; GH-N; GHN; hGH-N; Somatotropin; Hygetropin; Jintropin; Kigtropin; Pituitary Growth Hormone; Growth Hormone, Normal

ACTIVITY TEST

Growth Hormone (GH), also known as somatotropin, is a member of a family of growth factors. Human growth hormone is a 191 amino acid single-chain polypeptide produced via the anterior pituitary of the brain in the acidophilic, somatotrophic cells. Its production is tightly regulated through several complex feedback mechanisms in response to stress, exercise, nutrition, sleep, and growth hormone itself. Annexin A2 (ANXA2) has been shown to play multiple roles in growth, development, metabolism and ANXA2 is a high affinity receptor for GH. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human GH and recombinant human ANXA2. Briefly, GH was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to ANXA2-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-GH pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µl stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human GH and recombinant human ANXA2 was shown in Figure 1, the EC50 for this effect is 2.1 ug/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Neuroscience Letters The effect of peripheral administration of growth hormone on AD-like cognitive deficiency in NBM-lesioned rats PubMed: 19765635
Cellular Reprogramming Changes in the Somatotrophic Axis in Genetically Identical Dogs PubMed: 21740269
Clinical Cancer Research The efficacy of IGF-I receptor monoclonal antibody against human gastrointestinal carcinomas is independent of k-ras mutation status PubMed: 21642381
General and Comparative Endocrinology Growth hormone expression and neuroprotective activity in a quail neural retina cell line ScienceDirect: S0016648009002457
Nutrition and Metabolism Periodic 48 h feed withdrawal improves glucose tolerance in growing pigs by enhancing adipogenesis and lipogenesis Nutrition&Metabolism: source
Veterinary Recprd Case Report Treatment of cervical metaphyseal fractures due to delayed ossification in an adult dog with hypopituitarism Bmj: Source
Journal of the American Animal Hospital Association GH Deficiency in a Dalmatian Puppy with Megaesophagus Pubmed: 24051259
Biotechnology and Bioprocess Engineering Functional expression of bovine growth hormone gene in Pleurotus eryngii Springer: Source
Buletin Veteriner Udayana Profil Growth Hormone Sapi Bali Betina Pada Tiga Tipe Lahan Pemeliharaan Di Provinsi Bali Unud:Source
Veterinary World Effect of prill fat supplementation on hormones, milk production and energy metabolites during mid lactation in crossbred cows Veterinaryworld:Source
Int J Clin Exp Med Effects of sericin on the testicular growth hormone/insulin-like growth factor-1 axis in a rat model of type 2 diabetes PubMed: 26379831
Acta Histochemica Melatonin modulates monochromatic light-induced GHRH expression in the hypothalamus and GH secretion in chicks Pubmed:26948666
ANNALS OF ANIMAL SCIENCE Comparison of the effect of a standard inclusion level of inorganic zinc to organic form at lowered level on bone development in growing male Ross broiler chickens journalprintahead:87
Annals of Animal Science Comparison of the Effect of a Standard Inclusion Level of Inorganic Zinc to Organic Form at Lowered Level on Bone Development in Growing Male Ross Broiler Chickens view:87
Journal of Dairy Science Effect of thermal stress on physiological, hormonal and haematological parameters in Tharparkar and Karan Fries calves publication:306322503
Jurnal Riset Akuakultur TRANSMISI GEN PhGH DAN PERFORMA PERTUMBUHAN IKAN LELE AFRIKA (Clarias gariepinus) TRANSGENIK GENERASI KETIGA 1329
Trop Anim Health Prod Assessment of feeding varying levels of Metabolizable energy and protein on performance of transition Murrah buffaloes pubmed:28785907
Biological Trace Element Research Effect of Dietary Phytase Supplementation on Bone and Hyaline Cartilage Development of Broilers Fed with Organically Complexed Copper in a Cu-Deficient Diet article:10.1007
Revista Brasileira de Ciência Avícola Effect of Zinc Level and Source (Zinc Oxide Vs. Zinc Glycine) on Bone Mechanical and Geometric Parameters, and Histomorphology in Male Ross 308 Broiler Chicken 10.159:1806-9061-2016-0285
畜牧兽医学报 单色绿光对鸡胚褪黑激素合成的影响 DOI: 10.11843/j.issn.0366-6964.2017.05.019
Obesity Surgery Effects of Bariatric Surgery on Change of Brown Adipocyte Tissue and Energy Metabolism in Obese Mice pubmed:28853013
JURNAL VETERINER Kadar Hormon Pertumbuhan Sapi Bali Lebih Rendah di Nusa Penida Daripada Daerah Bali Lainnya(LEVELS OF GROWTH HORMONE BALI CATTLE IN NUSA PENIDA LOWER THAN OTHER BALI REGIONS) 10.19087/jveteriner.2017.18.2.226
Veterinary World. Factors influencing growth hormone levels of Bali cattle in Bali, Nusa Penida, and Sumbawa Islands, Indonesia. pubmed:29184372
Biochemistry, Genetics and Molecular Biology Effects of Dietary Lysine Levels on the Plasma Concentrations of Growth‐Related Hormones in Late‐Stage Finishing Pigs 10.5772/intechopen.68545
Cellular Physiology and Biochemistry Decreased Hepatic Lactotransferrin Induces Hepatic Steatosis in Chronic Non-Alcoholic Fatty Liver Disease Model Pubmed:29975946
PLoS One Subsequent somatic axis and bone tissue metabolism responses to a low-zinc diet with or without phytase inclusion in broiler chickens Pubmed:29373588
Pakistan Journal of Zoology Effects of Intrauterine Growth Retardation on Growth, Meat Quality and Muscle Fiber Composition of Pigs. :
Cell Reports Zika Virus Infection in Hypothalamus Causes Hormone Deficiencies and Leads to Irreversible Growth Delay and Memory Impairment in Mice Pubmed: 30404008
International Journal of Current Microbiology and Applied Sciences Physiological Performance of Crossbred Cattle Calves (Karan Fries) under Different Housing Conditions during Different Seasons
Photochemistry and Photobiology Melatonin Receptor Mel1b‐and Mel1c‐mediated Green Light Induced the Secretion of Growth Hormone in Anterior Pituitary of Chicks Pubmed: 31111492
Journal of Animal Science Dietary lysine affects amino acid metabolism and growth performance, which may not involve the GH/IGF-1 axis, in young growing pigs Pubmed: 31922564
SAUDI MEDICAL JOURNAL The evaluation of leukocyte-platelet rich fibrin as an anti-inflammatory autologous biological additive Pubmed: 31287125
Frontiers in Endocrinology RFRP-3, the Mammalian Ortholog of GnIH, Is a Novel Modulator Involved in Food Intake and Glucose Homeostasis Pubmed: 32328034
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION Isolated growth hormone deficiency in a Chihuahua with a GH1 mutation Pubmed: 32646299
Scientific Reports Controlled elevated temperatures during early-mid gestation cause placental insufficiency and implications for fetal growth in pregnant pigs 33244103
Animal Effect of alfalfa hay and starter feed supplementation on caecal microbiota and fermentation, growth, and health of yak calves 33610512
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE GHR is involved in gastric cell growth and apoptosis via PI3K/AKT signalling 33492754
animals Effects of Concentrate and Bypass Fat Supplementations on Growth Performance, Blood Profile, and Rearing Cost of Feedlot Buffaloes 34359233
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
APA044Hu01 Active Growth Hormone (GH) Cell culture; Activity Assays.
EPA044Hu61 Eukaryotic Growth Hormone (GH) Positive Control; Immunogen; SDS-PAGE; WB.
RPA044Hu01 Recombinant Growth Hormone (GH) Positive Control; Immunogen; SDS-PAGE; WB.
PAA044Hu06 Polyclonal Antibody to Growth Hormone (GH) WB; IHC; ICC; IP.
PAA044Hu01 Polyclonal Antibody to Growth Hormone (GH) WB; IHC; ICC; IP.
MAA044Hu22 Monoclonal Antibody to Growth Hormone (GH) WB; IHC; ICC; IP.
MAA044Hu26 Monoclonal Antibody to Growth Hormone (GH) WB; IHC; ICC; IP.
MAA044Hu21 Monoclonal Antibody to Growth Hormone (GH) WB; IHC; ICC; IP.
MAA044Hu23 Monoclonal Antibody to Growth Hormone (GH) WB; IHC; ICC; IP.
MAA044Hu24 Monoclonal Antibody to Growth Hormone (GH) WB; IHC; ICC; IP.
MAA044Hu25 Monoclonal Antibody to Growth Hormone (GH) WB; IHC; ICC; IP.
LAA044Hu72 Biotin-Linked Monoclonal Antibody to Growth Hormone (GH) WB; IHC; ICC.
SEA044Hu ELISA Kit for Growth Hormone (GH) Enzyme-linked immunosorbent assay for Antigen Detection.
CEA044Hu ELISA Kit for Growth Hormone (GH) Enzyme-linked immunosorbent assay for Antigen Detection.
AEA044Hu ELISA Kit for Anti-Growth Hormone Antibody (Anti-GH) Enzyme-linked immunosorbent assay for Antibody Detection.
SCA044Hu CLIA Kit for Growth Hormone (GH) Chemiluminescent immunoassay for Antigen Detection.
LMA044Hu Multiplex Assay Kit for Growth Hormone (GH) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
PSA044Hu01 Antibody Pair for Growth Hormone (GH) ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSA044Hu01 ELISA Kit DIY Materials for Growth Hormone (GH) Main materials for "Do It (ELISA Kit) Yourself".