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Active Fibroblast Growth Factor 9 (FGF9)

GAF; HBFG9; Glia-Activating Factor; Heparin-Binding Growth Factor 9

ACTIVITY TEST

Fibroblast Growth Factor 9 (FGF9) is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF9 was isolated as a secreted factor that exhibits a growth-stimulating effect on cultured glial cells. In nervous system, this protein is produced mainly by neurons and may be important for glial cell development. Besides, Fibroblast Growth Factor Receptor 1 (FGFR1) has been identified as an interactor of FGF9, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse FGF9 and recombinant mouse FGFR1. Briefly, FGF9 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100ul were then transferred to FGFR1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-FGF9 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution , wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of FGF9 and FGFR1 was shown in Figure 1, and this effect was in a dose dependent manner.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPA036Mu02 Recombinant Fibroblast Growth Factor 9 (FGF9) Positive Control; Immunogen; SDS-PAGE; WB.
APA036Mu01 Active Fibroblast Growth Factor 9 (FGF9) Cell culture; Activity Assays.
RPA036Mu01 Recombinant Fibroblast Growth Factor 9 (FGF9) Positive Control; Immunogen; SDS-PAGE; WB.
PAA036Mu02 Polyclonal Antibody to Fibroblast Growth Factor 9 (FGF9) WB; IHC; ICC; IP.
PAA036Mu01 Polyclonal Antibody to Fibroblast Growth Factor 9 (FGF9) WB; IHC; ICC; IP.
MEA036Mu Mini Samples ELISA Kit for Fibroblast Growth Factor 9 (FGF9) Enzyme-linked immunosorbent assay for Antigen Detection.
SEA036Mu ELISA Kit for Fibroblast Growth Factor 9 (FGF9) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA036Mu CLIA Kit for Fibroblast Growth Factor 9 (FGF9) Chemiluminescent immunoassay for Antigen Detection.
LMA036Mu Multiplex Assay Kit for Fibroblast Growth Factor 9 (FGF9) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.