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Active Factor Related Apoptosis Ligand (FASL)

CD178; CD95L; CD95-L; FASLG; FASLG; APT1LG1; APT1-LG1; TNFSF6; Fas Antigen Ligand; TNF Superfamily Member 6; Tumor Necrosis Factor(ligand)Superfamily,Member 6

ACTIVITY TEST

Fas ligand (FASL) is a 40 kDa type II membrane protein belonging to the TNF family, referred to as TNFSF6 in the new TNF superfamily nomenclature. Its specific receptor is FAS (CD95, Apo-1), a 45 kDa type I transmembrane protein of the TNF receptor family. FASL is mainly expressed on activated T cells and NK cells, while FAS is present on various cell types. The FAS/FASL system is crucial for regulating immune responses by inducing cell apoptosis, thereby maintaining homeostasis, lymphocyte self-tolerance, and immune privilege. FASL has also been reported as a potent neutrophil chemoattractant, indicating a new proinflammatory function. Additionally, a functional binding ELISA assay was used to detect the interaction between recombinant mouse FASL and recombinant human FAS.biotin-linked FASL were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100µl were then transferred to FAS-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µl stop solution to the wells and read at 450nm immediately. The binding activity of FASL and FAS was shown in Figure 1, the EC50 for this effect is 0.46 µg/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
PLoS Pathogens Macrophage-expressed IFN-β Contributes to Apoptotic Alveolar Epithelial Cell Injury in Severe Influenza Virus Pneumonia PubMed: PMC3585175
Tumor Biology The diplotype Fas? 1377A/? 670G as a genetic marker to predict a lower risk of breast cancer in Chinese women Springer:Source
IOS Press Content Library An Intranasal Formulation of Erythropoietin (Neuro-EPO) Prevents Memory Deficits and Amyloid Toxicity in the APPSwe Transgenic Mouse Model of Alzheimer's … articles:journal-of-alzheimers-disease
博士论文 INSTITUTO DE CIENCIAS BÁSICAS Y PRECLÍNICAS “VICTORIA DE GIRÓN” DEPARTAMENTO DE HISTOLOGÍA
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