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Polyclonal Antibody to Hypoxia Inducible Factor 1 Alpha (HIF1a)

HIF1-A; MOP1; PASD8; Basic Helix-Loop-Helix Transcription Factor; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against HIF1a. It has been selected for its ability to recognize HIF1a in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Cancer Chemotherapy and Pharmacology Quercetin greatly improved therapeutic index of doxorubicin against 4T1 breast cancer by its opposing effects on HIF-1α in tumor and normal cells. PubMed: 19466611
European Journal of Pharmacology Baicalin suppresses lung carcinoma and lung metastasis by SOD mimic and HIF-1α inhibition ScienceDirect: S0014299909011352
PLoS ONE Lactate Dehydrogenase 5 Expression in Non-Hodgkin Lymphoma Is Associated with the Induced Hypoxia Regulated Protein and Poor Prognosis PubMed: PMC3781153
International Journal of Molecular Medicine Selective inhibition of PKCβ2 preserves cardiac function after myocardial infarction and is associated with improved angiogenesis of ischemic myocardium in diabetic rats Pubmed: 24002353
Canadian Journal of Physiology and Pharmacology Modulation of arginine and asymmetric dimethylarginine concentrations in liver and plasma by exogenous hydrogen sulfide in LPS-induced endotoxemia Pubmed: 24289078
US8569372 B2 Method for preventing or treating a disease, disorder or condition induced by retina ischemia US8569372
J Surg Res. Effects of adrenomedullin and vascular endothelial growth factor on ischemia/reperfusion injury in skeletal muscle in rats. Pubmed: 23816242
PLoS One. HIF-1α Is Essential for Effective PMN Bacterial Killing, Antimicrobial Peptide Production and Apoptosis in Pseudomonas aeruginosa Keratitis Plos: Source
Journal of Physiological Anthropology Telomeres are elongated in rats exposed to moderate altitude Pubmed:Pmc4088304
Molecular Medicine Reports Resveratrol abrogates the effects of hypoxia on cell proliferation, invasion and EMT in osteosarcoma cells through downregulation of the HIF-1α protein. Pubmed:25384583
International Journal of Pharmaceutics The enhancement of gene silencing efficiency with chitosan-coated liposome formulations of siRNAs targeting HIF-1α and VEGF Pubmed:25445537
International Journal of Molecular Medicine Effect of combined treatment with rosuvastatin and protein kinase Cβ2 inhibitor on angiogenesis following myocardial infarction in diabetic rats Pubmed:25524396
International Journal of Developmental Neuroscience Maternal omega-3 fatty acid supplementation to a vitamin B 12 deficient diet normalizes angiogenic markers in the pup brain at birth PubMed: 25889224
Eur Surg Res Determination of the Preferred Conditions for the Isolated Perfusion of Porcine Kidneys PubMed: 25341590
Sci Rep Oxygen-induced cell migration and on-line monitoring biomarkers modulation of cervical cancers on a microfluidic system PubMed: 25905434
Evidence-Based Complementary and Alternative Medicine Metabolomic Analysis of Biochemical Changes in the Plasma of High-Fat Diet and Streptozotocin-Induced Diabetic Rats after Treatment with Isoflavones Extract of Radix Puerariae pmc:PMC4794592
Biol Trace Elem Res. Roles of Copper in Hepatocarcinogenesis via the Activation of Hypoxia-Inducible Factor-1α Pubmed:27121973
Раны и раневые инфекции. Использование гипоксического прекондиционирования для подготовки аутодермотрансплантата у больных со скомпрометированной микроциркуляцией 91
Biological Trace Element Research Roles of Copper in Hepatocarcinogenesis via the Activation of Hypoxia-Inducible Factor-1α. pubmed:27121973
Experimental and Therapeutic Medicine Expression of hypoxia-inducible factor-1α in synovial fluid and articular cartilage is associated with disease severity in knee osteoarthritis etm:13
Clinical Trials in Degenerative Diseases Correlating oxidative stress-related factors with bone metabolic markers in older adult male patients exhibiting degenerative osteoporosis in the high-altitude hypoxic area of China: study protocol for a non-randomized controlled trial issn:2542-3975
Annals of Clinical & Laboratory Science R132H Mutation in IDH1 Gene is Associated with Increased Tumor HIF1-Alpha and Serum VEGF Levels in Primary Glioblastoma Multiforme pubmed:28667042
Archives of Gynecology and Obstetrics Is there any relationship between adipocytokines and angiogenesis factors to address endothelial dysfunction and platelet aggregation in untreated patients with preeclampsia? 10.1007/s00404-017-4461-2
EXPERIMENTAL AND THERAPEUTIC MEDICINE Expression of hypoxia-inducible factor-1α in synovial fluid and articular cartilage is associated with disease severity in knee pubmed:28123469
Medical Journal Armed Forces India Study of acute hypoxia markers in healthy subjects: Utility in post-crash investigation 10.1016/j.mjafi.2017.04.003
Journal of Physiological Anthropology Telomere elongation protects heart and lung tissue cells from fatal damage in rats exposed to severe hypoxia Pubmed:29454386
Bulletin of Experimental Biology and Medicine Signal Mechanism of the Protective Effect of Combined Preconditioning by Amtizole and Moderate Hypoxia Pubmed:29308565
Microchimica Acta Determination of hypoxia-inducible factor-1 by using a ratiometric colorimetric test based on click-mediated growth of gold nanoparticles Pubmed: 30209641
Bulletin of Experimental Biology and Medicine Expression of Hif-1α, Nf-κb, and Vegf Genes in the Liver and Blood Serum Levels of HIF-1α, Erythropoietin, VEGF, TGF-β, 8-Isoprostane, and Corticosterone in Wistar Rats with High and Low Resistance to Hypoxia. Corticosterone in … Pubmed: 30353332
Translational Oncology OKN-007 Increases temozolomide (TMZ) Sensitivity and Suppresses TMZ-Resistant Glioblastoma (GBM) Tumor Growth Pubmed: 30468988
West Indian Medical Journal Plasma Cellular Hypoxia, Mitochondrial Dysfunction, Disease Risk and Prognostic Factors in Type 2 Diabetic Patients in Saudi Arabia.
Bulletin of Experimental Biology and Medicine Age-Specific Features of Hypoxia Tolerance and Intensity of Lipopolysaccharide-Induced Systemic Inflammatory Response in Wistar Rats Pubmed: 30903510
The role of amniotic fluid exosome, hypoxia inducible fator-1α, and inflammatory cytokine in cervical insufficiency
scandinavian journal of immunology Vitexin alleviates interleukin‐1β induced inflammatory responses in chondrocytes from osteoarthritis patients: involvement of HIF‐1α pathway Pubmed: 31055848
JOURNAL OF PERIODONTOLOGY A disintegrin‐like and metalloproteinase with thrombospondin‐1 (ADAMTS‐1) levels in gingival crevicular fluid correlate with vascular endothelial growth factor‐A … Pubmed: 31020669
Tissue Eng Part A Hypoxia Preconditioned Adipose Derived Endothelial Progenitor Cells Promote Bladder Augmentation Pubmed: 31238789
ПОТЕНЦИРОВАНИЕ АНТИГИПОКСАНТАМИ ЭФФЕКТА ГИПОКСИЧЕСКОГО ПРЕКОНДИЦИОНИРОВАНИЯ
molecular and cellular biochemistry Maternal omega-3 fatty acids and vitamin E improve placental angiogenesis in late-onset but not early-onset preeclampsia Pubmed: 31420792
Molecules Regulation of MicroRNA-155 and Its Related Genes Expression by Inositol Hexaphosphate in Colon Cancer Cells Pubmed: 31744065
Stem Cell Res Ther Short-wave enhances mesenchymal stem cell recruitment in fracture healing by increasing HIF-1 in callus Pubmed: 32894200
J Cell Physiol PLCε regulates metabolism and metastasis signaling via HIF‐1α/MEK/ERK pathway in prostate cancer Pubmed: 32383180
Гипоксия-зависимый транскрипционный контроль активности деструктивных изменений пародонта воспалительного и злокачественного генеза
Food Funct Prenatal vitamin D supplementation reduces blood pressure and improves placental angiogenesis in an animal model of preeclampsia 33237074
Eur J Pharmacol Lactucaxanthin protects retinal pigment epithelium from hyperglycemia-regulated hypoxia/ER stress/VEGF pathway mediated angiogenesis in ARPE-19 cell and rat ¡­ 33705802
EXPERIMENTAL EYE RESEARCH Astaxanthin mediated regulation of VEGF through HIF1¦Á and XBP1 signaling pathway: An insight from ARPE-19 cell and streptozotocin mediated diabetic rat model 33789142
scientific reports Serum hypoxia-inducible factor-1¦Á and uterine artery Doppler ultrasound during the first trimester for prediction of preeclampsia 33758274
Molecular Biology Reports Umbilical cord-derived mesenchymal stem cell conditioned medium reverses neuronal oxidative injury by inhibition of TRPM2 activation and the JNK signaling … Pubmed:35585377
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