Packages (Simulation)
Image (I)
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DAB staining on IHC-P; Samples: Human Kidney Tissue; Primary Ab: 20µg/ml Rabbit Anti-Human GAL1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Human Rectum Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human GAL1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Renal cancer Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human GAL1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Image (II)
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Figure. Western Blot; Sample: Recombinant GAL1, Human.
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Knockout Varification:
Lane 1: Wild-type K562 cell lysate;
Lane 2: GAL1 knockout K562 cell lysate;
Predicted MW: 15kd
Observed MW: 15kd
Primary Ab: 1µg/ml Rabbit Anti-Human GAL1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
K562 human chronic myelogenous leukemia cell line was stained with rabbit Anti-human GAL1 Polyclonal Antibody (Catalog # PAA321Hu01, filled red histogram) or Isotype control antibody (Catalog # IS067-Rb01, filled green histogram), followed by APC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog # SAA544Rb15). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.
Quality Guarantee
Instruction Manual
Certificate
- KO-Validated
Polyclonal Antibody to Galectin 1 (GAL1)
LGALS1; GBP; HLBP14; Galaptin; Lectin,Galactoside-Binding Soluble 1; 14 kDa laminin-binding protein; Lactose-binding lectin 1; Putative MAPK-activating protein PM12; S-Lac lectin
- Product No.PAA321Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPA321Hu01-Recombinant Galectin 1 (GAL1)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration0.47mg/ml
- Organism Species MoreMus musculus (Mouse), Rattus norvegicus (Rat)
- ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies. - DownloadInstruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
- FOB
US$ 95
For more details, please contact local distributors! US$ 221
For more details, please contact local distributors! US$ 316
For more details, please contact local distributors! US$ 790
For more details, please contact local distributors! US$ 3160
For more details, please contact local distributors!
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against GAL1. It has been selected for its ability to recognize GAL1 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.5-2µg/mL;1:230-900
Immunohistochemistry: 5-20µg/mL;1:23-90
Immunocytochemistry: 5-20µg/mL;1:23-90
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
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Antibody Labeling Customized Service
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Protein A/G Purification Column
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Staining Solution for Cells and Tissue
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Immunohistochemistry (IHC) Experiment Service
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Immunocytochemistry (ICC) Experiment Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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PBS Buffer
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DAB Kit
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SABC Kit
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Long-arm Biotin Labeling Kit
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Real Time PCR Experimental Service
| Magazine | Citations |
| International Journal of Oncology | Galectin-1-mediated cell adhesion, invasion and cell death in human anaplastic large cell lymphoma: Regulatory roles of cell surface glycans Pubmed:24589677 |
| Clinica Chimica Acta | Galectin-1 and galectin-3: plausible tumour markers for oral squamous cell carcinoma and suitable targets for screening high-risk population Pubmed:25578395 |
| Journal of Perinatal Medicine | Cord blood galectin-1 and-3 concentrations in term pregnancies with normal restricted and increased fetal growth PubMed: 24717333 |
| Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
| RPA321Hu01 | Recombinant Galectin 1 (GAL1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA321Hu01 | Polyclonal Antibody to Galectin 1 (GAL1) | WB; IHC; ICC; IP. |
| FAA321Hu01 | Anti-Galectin 1 (GAL1) Polyclonal Antibody | Flow cytometry. |
| LAA321Hu71 | Biotin-Linked Polyclonal Antibody to Galectin 1 (GAL1) | WB; IHC; ICC. |
| LAA321Hu81 | FITC-Linked Polyclonal Antibody to Galectin 1 (GAL1) | WB; IHC; ICC; IF. |
| MAA321Hu22 | Monoclonal Antibody to Galectin 1 (GAL1) | WB; IHC; ICC; IP. |
| SEA321Hu | ELISA Kit for Galectin 1 (GAL1) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA321Hu | CLIA Kit for Galectin 1 (GAL1) | Chemiluminescent immunoassay for Antigen Detection. |
| KSA321Hu01 | ELISA Kit DIY Materials for Galectin 1 (GAL1) | Main materials for "Do It (ELISA Kit) Yourself". |