Staining Solution for Cells and Tissue
First Edition (Revised on April, 2016)
The staining technology is one of the important methods to study the pathology. Pathology teaching, scientific research, clinical biopsy and diagnosis can not do without pathological staining technology. In order to display and determine the tissues or cells in the normal structure or in the pathological process of abnormal substances, diseases and pathogens. Appropriate staining methods are for different tissues and different purposes.
Products and application
|Product Name||Application||Section type||Format|
|Hematoxylin eosin (HE) staining solution||Hematoxylin stains the nucleus chromatin and cytoplasm ribosome with purple blue, eosin stains the cytoplasm and the extracellular matrix components with red.||Paraffin section Frozen section Cells||Hematoxylin solution: 100ML|
Eosin Solution: 100ML
|Masson staining solution||Masson staining was mainly used for the differential staining of collagen fibers and muscle fibers. Collagen and cartilage was blue, muscle fiber, cellulose and red blood cell stained red, nuclei stained blue black.||Paraffin section Frozen section||Weigert's iron hematoxylin A: 20ML|
Weigert's iron hematoxylin B: 20ML
Ponceau red Fuchsine Acid: 20ML
1% phosphomolybdic acid: 20ML
2% acetic acid aniline blue: 20ML
|Alcian blue and periodic acid||AB-PAS staining is used to classify the gastric mucosal||Paraffin section||Alcian blue solution: 20ML|
|Schiff (AB-PAS) staining solution||epithelial metaplasia of gastric mucosa, and to observe the changes of the morphology of glycogen and mucous substance. Acid mucus was blue, neutral mucus was red, mixed mucus was purple, the nucleus was pale blue||Frozen section||Schiff reagent: 20ML|
Periodic acid: 20ML
Mayer hematoxylin: 20ML
Sulfinic acid wash buffer: 100ML
|Alcian blue staining solution||Alcian blue belongs to cationic dyes, is the most specific dye for acid mucus.||Paraffin section Frozen section||Alcian blue（PH=2.5) solution: 100ML|
Nuclear Fast Red solution: 100ML
|Triphenyl tetrazolium chloride (TTC) staining solution||Triphenyl tetrazolium chloride (TTC) is a fat soluble light sensitive compound, and normal tissue in the dehydrogenase reaction were red, and tissue ischemia is pale.||Fresh tissue（such as brain tissue）||TTC solution: 100ML|
|Periodate Schiff (PAS) staining solution||PAS staining can show the polysaccharide, neutral mucus and some acidic mucus substances, as well as cartilage, pituitary, fungi, basement membrane and other substances.||Paraffin section Frozen section Cells||Schiff reagent: 20ML|
Mayer hematoxylin: 20ML
Sulfinic acid wash solution: 100ML
|Van Gieson (VG) staining solution||Van Gieson (VG) staining is used to distinguish between collagen fibers and muscle fibers. Staining solution is picric acid and acid fuchsin mixture, the collagen fibers were acid fuchsin stained pink or red, muscle fibers were dyed yellow picric acid||Paraffin section Frozen section||an Gieson A: 5ML|
Van Gieson B: 60ML
Weigert’s iron hematoxylin A: 20ML
Weigert’s iron hematoxylin B: 20ML
|Oil red O staining solution||Oil red O belongs to azo dyes, it is a strong fat solvent and dye, The fat droplets in the tissue are orange.||Frozen section Cells||Oil red O solution: 100ML|
|Annona Red-Fast Green staining solution||Annona Red-Fast Green staining is the common staining of articular cartilage, cartilage and bone tissue.||Paraffin section Frozen section||Annona Red: 100ML|
Fast Green: 100ML
1% phosphomolybdic acid: 100ML
Nucleic acid staining solution (DAPI)
DAPI stains the double-stranded DNA (dsDNA) with blue.
|Paraffin section Frozen section Cells|
Storage and Validity
Different components are stored in the 4°C, -20°C respectively, valid for one year.
1.Reagents should be stored according to the instructions.
2.Paraffin section/slice need complete dewaxing.
3.The time of periodate oxidation of tissue sections is within 10 minutes, the ambient temperature is not more than 20°C.