Elisa is a rapid, sensitive and accurate quantitative analysis method. It will need 100ul of sample for detection, while sometimes it’s just hard to get enough samples, especially when the concentration of target protein in the samples is low (such as: all kinds of cytokines). Then the normal ELISA kit will not be suitable. Now Cloud-Clone Corp. has developed Mini ELISA kits which require less samples (25ul~50ul), with the same procedure and experimental time, and its result is the same or even better than conventional kits. Now let’s take SEA079Mu (ELISA Kit for Interleukin 6 (IL6)) and MEA079Mu (Mini ELISA Kit for Interleukin 6 (IL6)) as an example to compare the whole process. T......2015-05-25

As we know, for antigens with multiple epitopes, double sandwich ELISA (Double Sandwich ELISA) can be applied to the quantitative detection of target antigens or antibodies. While for small molecule or hapten, as lacking of more than two binding sites for double sandwich detection, double sandwich method is not suitable. In this case, competitive inhibition method can be applied to detect target molecules. Competitive inhibition ELISA also can be called blocking ELISA, its main principle is the competive binding of the antigens within the samples and the certain amount of labeled-antigens with antibody on the solid-phase. The more amount of antigen in samples, the less labeled antigen ......2015-05-14

In ELISA experiments, researchers often encounter samples with low OD, including serum, plasma, tissue homogenate, cell lysate, etc. Assuming the customer's operation is right, the standard curve is also good, in this situation the reasons can be analyzed in two ways. On one hand, the content of the sample itself can be detected, but some operations cause the samples measured value become out of the detection range of Elisa kit; On the other hand, low concentration of analyte in the sample itself. Following we’ll analyze in these two aspects and raise their solutions. 1. When the content of target molecules itself can be detected, what factors may cause low measured value......2015-05-05

Since the 1970s, ELISA, as a simple and rapid method for the quantitative determination trace protein, has been widely used in many fields of biology and medicine. Enzyme linked immunosorbent assay (ELISA, Enzyme-Linked Immunosorbnent Assay) is a new immunoassay technique based on the solid-phased antigen or antibody and enzyme-labeled antigen or antibody binding. It can be divided into those four categories: direct ELISA, indirect ELISA, sandwich ELISA and competitive inhibition ELISA. And wherein, the most widely used method for quantitative detection of the antigen or antibody is double sandwich ELISA, which is obtained by a known antigen or antibody adsorbed on a solid phase carri......2015-05-05

An autoantibody is an antibody (a type of protein) produced by the immune system that is directed against one or more of the individual's own tissues, organs,cells and cellular components (including proteins and nucleic acids). Normally, immune cells can discriminate self from non-self. A normal body does not respond to its own antigens. This self-defense mechanism of immune system is called Immune Tolerance. The body maintain normal growth, development and survival by immune tolerance systerm. Once this system is destroyed, the immune system recognize one or more of the body's normal constituents as "non-self", and leading to production of pathological autoantibodi......2015-05-05

Tumor protein p53 (P53, TP53) is an important protein coded by TP53 gene, it could inhibit tumor through regulation of cell cycle. Previously the main research directions on p53 are the function and mechanism in tumor inhibition. Previous studies showed that the calculated molecular weight of p53 is 43.7kDa, according to the amino acids of the protein, but the molecular weight (MW) shown in SDS-PAGE is 53kDa. The reason leads to this difference in molecular weight lies in the abundance of proline residue in the protein, and this phenomenon shows not only in human p53, but also in p53 of other species, including rat, mouse, frog and so on. The MW difference is in accordance with the p......2015-04-22