Obesity is the major risk factor for cardiovascular disease, but its mechanism is still not clear. Researchers have proposed two possible hypotheses on obesity-induced diseases: inflammation and increase of oxidative stress. The study of obesity mechanism depends on the study of obesity related bio-markers, and it is very important to find the optimal bio-markers to improve the accuracy of cardiovascular diseases diagnosis. The common used bio-markers are inflammation related bio-markers, for instance, IL6, Adiponectin, Leptin, etc, and oxidative stress related bio-markers, for instance, oxLDL, PPARg, MCP1, etc. Cloud-Clone Corp. has developed a series of quantitative ELISA kits for......2014-03-13

The homology of certain protein in different species is very high, from 95% to 100%. For example, actin beta(ACTb), which is the main component of cytoskeleton microfilament, is widely distributed in various tissues. The protein is expressed by housekeeping gene, and its highly conservative, it shares 100% homology in different mammal species, such as human, rat, mouse, rabbit, pig, bovine, sheep, etc. Based on the characteristics of this kind of protein, we know when an antibody could identify one protein, then it can recognize and combine with this protein in different species. As for this phenomenon, Cloud-Clone Corp. developes a new type of ELISA detection kit with antibody whic......2014-02-08

Calprotectin, usually known as S100A8/A9, is a heterodimeric complex composed by two components, S100A8 and S100A9. These two proteins are calcium- and zinc-binding proteins from the S100 protein family that are released from neutrophils under inflammatory conditions. It has been reported that amounts of S100A8/A9 in blood or extracellular body fluid are increased under many pathological conditions, for instance, rheumatoid arthritis[1], inflammatory bowel diseases[2], viral or microbial infections[3,4], tumors[5], and many inflammatory conditions[6]. Notably, abscess fluids contain more than 1mg/mL of the protein complex[7]. Therefore, the measurement of Calprotectin could be consider......2013-07-25

Gene is the basis of molecular biology. Plasmid construction always includes primer design, template preparation, PCR amplification, fragment recovery, enzyme digestion and ligation, transformation and the final sequencing. The whole process usually takes two to three weeks to complete. However, for each specific gene fragment, many factors should be considered such as restriction enzyme sites. Non-specific bands should also be avoided including Hairpin, False priming and Cross Dimer formed by sense/antisense-primer. In addition, annealing temperature and reagent ratio system should be optimized though repeated tests. RNA extraction and reverse transcription of cDNA are needed for t......2013-07-24

Calprotectin, usually known as S100A8/A9, is a heterodimeric complex composed by two components, S100A8 and Mature collagen molecule has a structure of 3 short non-helix peptide chains at its N-terminal (N terminal) and carboxyl terminal (C terminal). It is called terminal peptide (Telopeptide). Type I collagen cross-linked N-telopeptide (NTX) and type I collagen cross-linked C-terminal peptide (CTX) are widely used as markers for collagen degradation. Both of NTX and CTX can be detected in serum and urine. Wherein the urinary NTX (Cross Linked N-Telopeptide Of Type I Collagen, cE90639Hu) is the direct product of type I collagen degradation by osteoclast. Not only could NTX be used t......2013-07-09

Recombinant protein technology is one of the most widely used biotechnology to obtain target protein. Protein purification is the most critical and difficult step in recombinant protein technology. The quality of the target proteins depends on the step of purification. Currently, the conventional method is to fuse target protein and an affinity purification label (Tag), the target protein can be purified through affinity purification of the tag. In addition, how to detect the expression of target protein is also a big problem for biologists. It is obviously complicated and expensive to use different antibodies to detect different target proteins. Sometimes it is even impossible to f......2013-07-09