Western blot (Western Blot) is a rapid and sensitive technique for detecting the protein of interest in the samples according to the antigen-antibody reactions. Western blot protocol uses gel electrophoresis (SDS-PAGE or native PAGE) to separate proteins in samples based on the difference of molecular weight of proteins . Then the proteins are transferred from the gel onto a membrane (typically nitrocellulose or PVDF), which is non-covalently bound to the proteins and can maintain the biological activity of proteins. After that, the membrane is probed using a primary antibody to detect the protein of interest followed by a further incubation with a secondary antibody conjugated to a re......2015-08-12

Elisa is a rapid, sensitive, accurate and reliable quantitative analysis method; sample preparation plays a pivotal role in a successful Elisa experiment. Collecting time, processing and storage all have influences on Elisa. Last time we introduce the common samples preparation including blood (serum, plasma), tissue homogenates, cell lysate and cell culture supernatant. This time we’ll present unusual samples such as skin, urine, feces, bronchoalveolar lavage fluid, saliva, cerebrospinal fluid, pleural effusion, prostatic fluid, semen, vaginal secretions, etc. for the reference. 1. Sputum Sputum must be treated before assaying according to the following directions. Mix the spu......2015-07-16

It is well known that there are three types of commonly used systems in the field of gene engineering, including prokaryotic expression system (Escherichia coli expression system, also called E. coli expression system), yeast expression system (Pichia P. expression system) and mammalian cell expression system. The prokaryotic expression system is widely used because of the advantages of simple operation, low cost and high expression. But at the same time, prokaryotic expression system also has some shortcomings, such as it is prone to form of inclusion body, has no posttranslational processing and so on In order to avoid the shortage of prokaryotic expression system itself, Cloud-Clo......2015-07-16

It is reported by World Health Organization (WHO) that cardiovascular disease has become the leading cause of death. From the statistical data of 2008, death caused by cardiovascular disease is 30% of global death toll, and it is predicted that the ratio will increased by 18% in 2030. Due to the significant impact of this diseases on human being, researchers all over the world had pay persistent attention on it. Recently, lots of reports, which are about a group of peptides, angiotesin (ANG) , are published. Regarding to these peptides, our company had developed related reagents, so, they could be used by researchers to facilitate their research. ANGs is a group of hormone consist of ......2015-07-16

ELISAis a rapid,sensitive, accurate and reliable quantitative analysis method. Samplepreparation plays a pivotal role in a successful ELISAexperiment. At the beginning of collecting sample for ELISA, we might have a few problems. The common samples used for ELISA include blood(serum, plasma), tissue homogenates, cell lysatesand cell culture supernatants, etc. Manyfactors will have influence on the results of ELISA experiment, such as sampling time, processing, storage, etc.Now we introduce sample processing methods for reference.1.BloodAfter collecting, blood should be processed as soon as possible to separate serum (plasma) from whole blood.What’s the difference between serum and pl......2015-05-26

Elisa is a rapid, sensitive and accurate quantitative analysis method. It will need 100ul of sample for detection, while sometimes it’s just hard to get enough samples, especially when the concentration of target protein in the samples is low (such as: all kinds of cytokines). Then the normal ELISA kit will not be suitable. Now Cloud-Clone Corp. has developed Mini ELISA kits which require less samples (25ul~50ul), with the same procedure and experimental time, and its result is the same or even better than conventional kits. Now let’s take SEA079Mu (ELISA Kit for Interleukin 6 (IL6)) and MEA079Mu (Mini ELISA Kit for Interleukin 6 (IL6)) as an example to compare the whole process. T......2015-05-25