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A brief introduction on Collagen types characteristics and detection methods
Collagen, mainly found in animal connective tissues, is the most abundant mammal protein, accounting for 25-35% of the total body protein content. Collagen can be divided into fiber type and non-fiber type. The most common types are Type I, Type II, Type III, Type IV, Type V. Wherein Type I, II, III, V are fibrous, Type IV is non-fibrous. So far 28 kinds of collagen are found, while over 90% are Type I. Collagen can be used for cosmetic surgery, bone grafts, tissue regeneration, wound healing, etc. Further, collagen mutations lead to diseases, including osteogenesis imperfecta, achondroplasia, osteoporosis and so on. Consequently, research on collagen has significant meanings, and ha......
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Good News! Cloud-Clone Corp.begins to provide IHC service!
Immunohistochemistry can be used for qualitative and semi-quantitative detection, aswell as location of antigen in situ in cells of a tissue section. Immunohistochemistry has the advantage of higher sensitivity, it is one of the most common immunological detection methods. However, due to the complicated procedures, such as antigen retrieval, determination of antibody’s concentration and appropriate staining, it always will lead to unsatisfied results. By popular demand, Cloud-Clone Corp. begins to provide IHC service for customer. IHC protocols Advantages There is a huge storage of semi-finished materials, more than 19,000 kinds of antibodies could be sent out immediately; ......
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Common Labeled Types of Antibodies, Better for Immunoassays
Antibody labeling is a common technology used in sample preparation for immunoelectron microscopy. Due to the simple operation, high sensitivity, it is widely used in various immunoassays such as IHC, WB, ICC, ELISA. The labels can amplify the detection signal for antigens, making it a fast and economic detection technology in the market. Commonly used molecules for antibody labeling include enzymes, fluorescent molecules, isotope, biotin, colloidal gold etc. Below is the introduction of conjugated antibodies from Cloud-Clone Corp. 1. Horseradish peroxidase (HRP) labeling. HRP is conjugated to monoclonal/polyclonal antibody by periodate method. The glycosyl of HRP is oxidized into al......
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The detection of the APOB family and its receptors
Human Apolipoprotein B (APOB ) was coded by APOB gene, and APOB is the primary apolipoprotein of very low-density lipoproteins(VLDL) and intermediate density lipoprotein(IDL). The levels of APOB are related to lipid metabolism, atherosclerosis and other cerebrovascular diseases. For this reason, it is the hot spot in Clinical research. While it is unclear what functional role APOB plays in low-density lipoproteins(LDL).The protein occurs in the plasma in 2 main isoforms, ApoB48 and ApoB100. The first is synthesized exclusively by the small intestine, the second by the liver. ApoB100 is the largest of the ApoB group of proteins, consisting of 4563 amino acids. In fact, ApoB48 is so call......
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Certificate of Analysis--customized labeled peptide
[ PROUDUCT ] Lot.No.: P20151130397 Protein Name: CKGGRAKDC tagged to rhodamine Size: 1mg [ QC TESTING ] Relative abundance of peptide is list bellow: Spectrum peak of labled peptide is list bellow: Relative abundance of labled peptide is list bellow:
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Principle for Immunohistochemistry (IHC) Assay
Immunohistochemistry (IHC) is also called as immunocytochemistry (ICC). It is an immunological method for qualitative and quantitative detection, as well as location of antigen in situ in cells of a tissue section, using specific and labeled antibody. Below is the principle: