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Wide-range ELISA Kit for Fibroblast Growth Factor 21 (FGF21)
- Product No.WEC918Ra
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length3h
- Detection Range12.35-1,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 4.77pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
- FOB
US$ 505
For more details, please contact local distributors! US$ 722 US$ 3249 US$ 6137 US$ 50540
Specificity
This assay has high sensitivity and excellent specificity for detection of Wide-range Fibroblast Growth Factor 21 (FGF21).
No significant cross-reactivity or interference between Wide-range Fibroblast Growth Factor 21 (FGF21) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Wide-range Fibroblast Growth Factor 21 (FGF21) and the recovery rates were calculated by comparing the measured value to the expected amount of Wide-range Fibroblast Growth Factor 21 (FGF21) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 87-103 | 97 |
EDTA plasma(n=5) | 98-105 | 101 |
heparin plasma(n=5) | 78-99 | 91 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wide-range Fibroblast Growth Factor 21 (FGF21) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wide-range Fibroblast Growth Factor 21 (FGF21) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Wide-range Fibroblast Growth Factor 21 (FGF21) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 85-97% | 88-96% | 99-105% | 88-96% |
EDTA plasma(n=5) | 78-94% | 79-103% | 96-105% | 91-101% |
heparin plasma(n=5) | 90-104% | 81-90% | 94-103% | 87-95% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
Standard | 2 | Standard Diluent | 1×20mL |
Detection Reagent A | 1 | Assay Diluent A | 1×12mL |
Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
Reagent Diluent | 1×300µL | Stop Solution | 1×6mL |
TMB Substrate | 1×9mL | Instruction manual | 1 |
Wash Buffer (30 × concentrate) | 1×20mL |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
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Magazine | Citations |
Lipids in Health and Disease | Dietary moderately oxidized oil induces expression of fibroblast growth factor 21 in the liver of pigs Lipidworld: source |
Endocrinology | Orphan nuclear receptor Nur77 mediates fasting-induced hepatic fibroblast growth factor 21 expression Endocrine:Source |
Biochemical and Biophysical Research Communications | Alpha lipoic acid induces hepatic fibroblast growth factor 21 expression via up-regulation of CREBH Pubmed:25449271 |
Free Radic Biol Med | Skeletal muscle increases FGF21 expression in mitochondrial disorders to compensate for energy metabolic insufficiency by activating the mTOR–YY1–PGC1α pathway PubMed: 25843656 |
Journal of diabetes and its complications | Associations between FGF21, osteonectin and bone turnover markers in type 2 diabetic patients with albuminuria. pubmed:27916484 |
American journal of the medical sciences | Association of Bone Metabolic Markers With Diabetic Retinopathy and Diabetic Macular Edema in Elderly Chinese Individuals With Type 2 Diabetes Mellitus pubmed:29078839 |
Frontiers in Physiology | Ileal Transposition Surgery Decreases Fat Mass and Improves Glucose Metabolism in Diabetic GK Rats: Possible Involvement of FGF21 Pubmed:29593555 |
Некоторые аспекты транспортной системы метаболизма железа в зависимости от степени синдрома перегрузки железом у детей с хроническим … | |
Physiol Res | Fibroblast growth factor 21 (FGF21) in children and adolescents with chronic kidney disease. Pubmed: 32469231 |
Chinese Journal of Osteoporosis | 2 型糖尿病患者血清FGF21, 骨连结蛋白和骨转换指标的相关性研究. |
Sci Rep | Ileal transposition helps to regulate plasma hepatokine levels in obese Zucker (Crl: ZUC (ORL)-Lepr fa) rats 33833309 |
Nutrients | The Speed of Ingestion of a Sugary Beverage Has an Effect on the Acute Metabolic Response to Fructose |
Medicine (Baltimore) | Association of serum fibroblast growth factor 21 with kidney function in a population-based Chinese cohort 34918690 |
Annals of Agricultural and Environmental Medicine | Oxidative stress-induced growth inhibitor 1 in alcohol-induced liver cirrhosis 34969228 |
J Magn Reson Imaging | Water Specific MRI T1 Mapping for Evaluating Liver Inflammation Activity Grades in Rats With Methionine‐Choline‐Deficient Diet‐Induced Nonalcoholic Fatty Liver … Pubmed:35212074 |
Lab Invest | Fibroblast growth factor 21, a stress regulator, inhibits Drp1 activation to alleviate skeletal muscle ischemia/reperfusion injury Pubmed:35488034 |
Molecular Biology Reports | Umbilical cord-derived mesenchymal stem cell conditioned medium reverses neuronal oxidative injury by inhibition of TRPM2 activation and the JNK signaling … Pubmed:35585377 |
High Circulating Fibroblast Growth Factor-21 Levels as a Prognostic Marker in Fatty Pancreas Patients |
Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
APC918Ra01 | Active Fibroblast Growth Factor 21 (FGF21) | Cell culture; Activity Assays. |
RPC918Ra02 | Recombinant Fibroblast Growth Factor 21 (FGF21) | Positive Control; Immunogen; SDS-PAGE; WB. |
EPC918Ra61 | Eukaryotic Fibroblast Growth Factor 21 (FGF21) | Positive Control; Immunogen; SDS-PAGE; WB. |
APC918Ra61 | Active Fibroblast Growth Factor 21 (FGF21) | Cell culture; Activity Assays. |
RPC918Ra01 | Recombinant Fibroblast Growth Factor 21 (FGF21) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAC918Ra02 | Polyclonal Antibody to Fibroblast Growth Factor 21 (FGF21) | WB; IHC; ICC; IP. |
PAC918Ra01 | Polyclonal Antibody to Fibroblast Growth Factor 21 (FGF21) | WB; IHC; ICC; IP. |
MAC918Ra21 | Monoclonal Antibody to Fibroblast Growth Factor 21 (FGF21) | WB; IHC; ICC; IP. |
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CEC918Ra | ELISA Kit for Fibroblast Growth Factor 21 (FGF21) | Enzyme-linked immunosorbent assay for Antigen Detection. |
WEC918Ra | Wide-range ELISA Kit for Fibroblast Growth Factor 21 (FGF21) | Enzyme-linked immunosorbent assay for Antigen Detection. |
CCC918Ra | CLIA Kit for Fibroblast Growth Factor 21 (FGF21) | Chemiluminescent immunoassay for Antigen Detection. |
LMC918Ra | Multiplex Assay Kit for Fibroblast Growth Factor 21 (FGF21) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
PGC918Ra01 | Primer Pair for Fibroblast Growth Factor 21 (FGF21) | PCR |