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Multiplex Assay Kit for Fibroblast Growth Factor 21 (FGF21) ,etc. by FLIA (Flow Luminescence Immunoassay)
(Note: Up to 8-plex in one testing reaction)
- Product No.LMC918Ra
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length1.5h
- Detection Range0.2-200pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.067 pg/mL.
- DownloadInstruction Manual
- UOM 8Plex 7Plex 6Plex 5Plex 4Plex 3Plex 2Plex1Plex
- FOB
US$ 474
US$ 492
US$ 520
US$ 556
US$ 593
US$ 648
US$ 730
Result
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Specificity
This assay has high sensitivity and excellent specificity for detection of Fibroblast Growth Factor 21 (FGF21) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Fibroblast Growth Factor 21 (FGF21) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Fibroblast Growth Factor 21 (FGF21) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Fibroblast Growth Factor 21 (FGF21) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 97-105 | 101 |
EDTA plasma(n=5) | 92-101 | 97 |
heparin plasma(n=5) | 78-89 | 86 |
sodium citrate plasma(n=5) | 88-96 | 91 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Growth Factor 21 (FGF21) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Growth Factor 21 (FGF21) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Fibroblast Growth Factor 21 (FGF21) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 90-97% | 81-99% | 97-105% | 88-101% |
EDTA plasma(n=5) | 85-95% | 82-93% | 85-104% | 84-93% |
heparin plasma(n=5) | 83-101% | 87-95% | 85-93% | 96-104% |
sodium citrate plasma(n=5) | 96-105% | 94-101% | 95-104% | 89-101% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
96-well plate | 1 | Plate sealer for 96 wells | 4 |
Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
Pre-Mixed Magnetic beads (22#:FGF21) | 1 | Analysis buffer | 1×20mL |
Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
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Magazine | Citations |
Lipids in Health and Disease | Dietary moderately oxidized oil induces expression of fibroblast growth factor 21 in the liver of pigs Lipidworld: source |
Endocrinology | Orphan nuclear receptor Nur77 mediates fasting-induced hepatic fibroblast growth factor 21 expression Endocrine:Source |
Biochemical and Biophysical Research Communications | Alpha lipoic acid induces hepatic fibroblast growth factor 21 expression via up-regulation of CREBH Pubmed:25449271 |
Free Radic Biol Med | Skeletal muscle increases FGF21 expression in mitochondrial disorders to compensate for energy metabolic insufficiency by activating the mTOR–YY1–PGC1α pathway PubMed: 25843656 |
Journal of diabetes and its complications | Associations between FGF21, osteonectin and bone turnover markers in type 2 diabetic patients with albuminuria. pubmed:27916484 |
American journal of the medical sciences | Association of Bone Metabolic Markers With Diabetic Retinopathy and Diabetic Macular Edema in Elderly Chinese Individuals With Type 2 Diabetes Mellitus pubmed:29078839 |
Frontiers in Physiology | Ileal Transposition Surgery Decreases Fat Mass and Improves Glucose Metabolism in Diabetic GK Rats: Possible Involvement of FGF21 Pubmed:29593555 |
Некоторые аспекты транспортной системы метаболизма железа в зависимости от степени синдрома перегрузки железом у детей с хроническим … | |
Physiol Res | Fibroblast growth factor 21 (FGF21) in children and adolescents with chronic kidney disease. Pubmed: 32469231 |
Chinese Journal of Osteoporosis | 2 型糖尿病患者血清FGF21, 骨连结蛋白和骨转换指标的相关性研究. |
Sci Rep | Ileal transposition helps to regulate plasma hepatokine levels in obese Zucker (Crl: ZUC (ORL)-Lepr fa) rats 33833309 |
Nutrients | The Speed of Ingestion of a Sugary Beverage Has an Effect on the Acute Metabolic Response to Fructose |
Medicine (Baltimore) | Association of serum fibroblast growth factor 21 with kidney function in a population-based Chinese cohort 34918690 |
Annals of Agricultural and Environmental Medicine | Oxidative stress-induced growth inhibitor 1 in alcohol-induced liver cirrhosis 34969228 |
J Magn Reson Imaging | Water Specific MRI T1 Mapping for Evaluating Liver Inflammation Activity Grades in Rats With Methionine‐Choline‐Deficient Diet‐Induced Nonalcoholic Fatty Liver … Pubmed:35212074 |
Lab Invest | Fibroblast growth factor 21, a stress regulator, inhibits Drp1 activation to alleviate skeletal muscle ischemia/reperfusion injury Pubmed:35488034 |
Molecular Biology Reports | Umbilical cord-derived mesenchymal stem cell conditioned medium reverses neuronal oxidative injury by inhibition of TRPM2 activation and the JNK signaling … Pubmed:35585377 |
High Circulating Fibroblast Growth Factor-21 Levels as a Prognostic Marker in Fatty Pancreas Patients |
Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
APC918Ra01 | Active Fibroblast Growth Factor 21 (FGF21) | Cell culture; Activity Assays. |
RPC918Ra02 | Recombinant Fibroblast Growth Factor 21 (FGF21) | Positive Control; Immunogen; SDS-PAGE; WB. |
EPC918Ra61 | Eukaryotic Fibroblast Growth Factor 21 (FGF21) | Positive Control; Immunogen; SDS-PAGE; WB. |
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PAC918Ra02 | Polyclonal Antibody to Fibroblast Growth Factor 21 (FGF21) | WB; IHC; ICC; IP. |
PAC918Ra01 | Polyclonal Antibody to Fibroblast Growth Factor 21 (FGF21) | WB; IHC; ICC; IP. |
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CEC918Ra | ELISA Kit for Fibroblast Growth Factor 21 (FGF21) | Enzyme-linked immunosorbent assay for Antigen Detection. |
WEC918Ra | Wide-range ELISA Kit for Fibroblast Growth Factor 21 (FGF21) | Enzyme-linked immunosorbent assay for Antigen Detection. |
CCC918Ra | CLIA Kit for Fibroblast Growth Factor 21 (FGF21) | Chemiluminescent immunoassay for Antigen Detection. |
LMC918Ra | Multiplex Assay Kit for Fibroblast Growth Factor 21 (FGF21) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
PGC918Ra01 | Primer Pair for Fibroblast Growth Factor 21 (FGF21) | PCR |