ELISA Kit for Beclin 1 (BECN1)

ATG6; VPS30; Coiled-Coil,Myosin-Like BCL2 Interacting Protein; ATG6 Autophagy Related 6 Homolog

Specificity

This assay has high sensitivity and excellent specificity for detection of Beclin 1 (BECN1).
No significant cross-reactivity or interference between Beclin 1 (BECN1) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Beclin 1 (BECN1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Beclin 1 (BECN1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Rejuvenation Res. Can Enhanced Autophagy be Associated with Human Longevity? Serum Levels of the Autophagy Biomarker Beclin-1 are Increased in Healthy Centenarians Pubmed:25307612
J Cosmet Dermatol A peel-off facial mask comprising myoinositol and trehalose-loaded liposomes improves adult female acne by reducing local hyperandrogenism and activating autophagy pubmed:28342238
Tumor Biology The novel CXCR4 antagonist, PRX177561, reduces tumor cell proliferation and accelerates cancer stem cell differentiation in glioblastoma preclinical models pubmed:28639900
19 Beclin1 circulating levels and accelerated ageing markers in COPD  DOI: 10.1183/1393003.congress-2017.PA1018
Cell Death & Disease Beclin1 circulating levels and accelerated aging markers in COPD Pubmed:29402890
Clinica Chimica Acta A novel combined score of biomarkers in sputum may be an indicator for lung cancer: A pilot study Doi: 10.1016/j.cca.2018.09.027
International Journal of Clinical and Experimental Pathology Serum concentration of autophagy-related protein 7 is correlated with non-alcoholic fatty liver disease in Chinese adults
EXPERIMENTAL AND THERAPEUTIC MEDICINE Crosstalk between vitamin D axis, inflammation and host immunity mechanisms: A prospective study 33936265
International Journal for Research in Applied Sciences and Biotechnology Inhibition of HepG-2 Cells (Liver Cancer Cell Line) Viability by 3-Hydroxypyridine-2-Carboxaldehyde N (4)-Methylthiosemicarbazone
BIOMARKERS OF AUTOPHAGY AND ANTIVIRAL ROLE OF PLACENTA FOR PREDICTING PRETERM DELIVERY IN PATIENTS WITH PRETERM DELIVERY?¡­
European Journal of Natural History IDENTIFICATION OF FACTORS CONNECTED WITH AUTOPHAGY ACTIVITY UNDER CONDITIONS OF PARTIAL NUTRITIONAL DEPRIVATION IN MEN
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPJ557Hu01 Recombinant Beclin 1 (BECN1) Positive Control; Immunogen; SDS-PAGE; WB.
PAJ557Hu01 Polyclonal Antibody to Beclin 1 (BECN1) WB; IHC
MAJ557Hu21 Monoclonal Antibody to Beclin 1 (BECN1) WB; IHC; ICC; IP.
MAJ557Hu22 Monoclonal Antibody to Beclin 1 (BECN1) WB; IHC; ICC; IP.
MAJ557Hu23 Monoclonal Antibody to Beclin 1 (BECN1) WB; IHC; ICC; IP.
MAJ557Hu24 Monoclonal Antibody to Beclin 1 (BECN1) WB; IHC; ICC; IP.
MAJ557Hu25 Monoclonal Antibody to Beclin 1 (BECN1) WB; IHC; ICC; IP.
SEJ557Hu ELISA Kit for Beclin 1 (BECN1) Enzyme-linked immunosorbent assay for Antigen Detection.
LMJ557Hu Multiplex Assay Kit for Beclin 1 (BECN1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.