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CLIA Kit for Antithrombin (AT)
AT3; ATIII; SERPINC1; Anti-Thrombin Antibodies; Serpin Peptidase Inhibitor Clade C Member 1; Coding Sequence Signal Peptide Antithrombin Part 1
- Product No.SCC313Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample TypePlasma
- Test MethodDouble-antibody Sandwich
- Assay Length2h, 40min
- Detection Range5.49-4,000ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 2.24ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
- FOB
US$ 588
For more details, please contact local distributors! US$ 840 US$ 3780 US$ 7140 US$ 58800
Specificity
This assay has high sensitivity and excellent specificity for detection of Antithrombin (AT).
No significant cross-reactivity or interference between Antithrombin (AT) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Antithrombin (AT) and the recovery rates were calculated by comparing the measured value to the expected amount of Antithrombin (AT) in samples.
Matrix | Recovery range (%) | Average(%) |
sodium citrate plasma(n=5) | 78-92 | 81 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Antithrombin (AT) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Antithrombin (AT) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Antithrombin (AT) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
sodium citrate plasma(n=5) | 92-99% | 83-90% | 85-101% | 88-104% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
Standard | 2 | Standard Diluent | 1×20mL |
Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
Substrate A | 1×10mL | Substrate B | 1×2mL |
Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.
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Magazine | Citations |
Thromb Res | Thalidomide and multiple myeloma serum synergistically induce a hemostatic imbalance in endothelial cells in vitro PubMed: 25840743 |
Neurotherapeutics | iTRAQ-Based Quantitative Proteomics Reveals the New Evidence Base for Traumatic Brain Injury Treated with Targeted Temperature Management pubmed:29247448 |
Journal of Clinical and Aesthetic Dermatology | Detection of Angiotensin II and AT1 Receptor Concentrations in Keloid and Hypertrophic Scar. |
博士论文 | An Agile Approach to Novel Recombinant Antithrombin Production for use in Biologics Development |
Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
NPC313Hu01 | Native Antithrombin (AT) | Positive Control; Immunogen; SDS-PAGE; WB. |
APC313Hu01 | Active Antithrombin (AT) | Cell culture; Activity Assays. |
RPC313Hu03 | Recombinant Antithrombin (AT) | Positive Control; Immunogen; SDS-PAGE; WB. |
EPC313Hu61 | Eukaryotic Antithrombin (AT) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPC313Hu01 | Recombinant Antithrombin (AT) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPC313Hu02 | Recombinant Antithrombin (AT) | Positive Control; Immunogen; SDS-PAGE; WB. |
APC313Hu61 | Active Antithrombin (AT) | Cell culture; Activity Assays. |
PAC313Hu01 | Polyclonal Antibody to Antithrombin (AT) | WB; IHC; ICC; IP. |
PAC313Hu02 | Polyclonal Antibody to Antithrombin (AT) | WB; IHC; ICC; IP. |
PAC313Hu03 | Polyclonal Antibody to Antithrombin (AT) | WB; IHC; ICC; IP. |
LAC313Hu81 | FITC-Linked Polyclonal Antibody to Antithrombin (AT) | WB; IHC; ICC; IF. |
LAC313Hu71 | Biotin-Linked Polyclonal Antibody to Antithrombin (AT) | WB; IHC; ICC. |
MAC313Hu21 | Monoclonal Antibody to Antithrombin (AT) | WB; IHC; ICC; IP. |
MAC313Hu27 | Monoclonal Antibody to Antithrombin (AT) | WB,IHC |
MAC313Hu29 | Monoclonal Antibody to Antithrombin (AT) | WB; IHC; ICC; IP. |
MAC313Hu23 | Monoclonal Antibody to Antithrombin (AT) | WB; IHC; ICC; IP. |
MAC313Hu24 | Monoclonal Antibody to Antithrombin (AT) | WB; IHC; ICC; IP. |
MAC313Hu26 | Monoclonal Antibody to Antithrombin (AT) | WB; IHC; ICC; IP. |
MAC313Hu22 | Monoclonal Antibody to Antithrombin (AT) | WB,IHC |
MAC313Hu28 | Monoclonal Antibody to Antithrombin (AT) | WB; IHC; ICC; IP. |
MAC313Hu25 | Monoclonal Antibody to Antithrombin (AT) | WB; IHC; ICC; IP. |
FAC313Hu02 | Anti-Antithrombin (AT) Monoclonal Antibody | FCM |
FAC313Hu04 | Anti-Antithrombin (AT) Monoclonal Antibody | FCM |
FAC313Hu08 | Anti-Antithrombin (AT) Monoclonal Antibody | FCM |
LAC313Hu72 | Biotin-Linked Monoclonal Antibody to Antithrombin (AT) | WB; IHC; ICC. |
LAC313Hu82 | FITC-Linked Monoclonal Antibody to Antithrombin (AT) | WB; IHC; ICC; IF. |
SEC313Hu | ELISA Kit for Antithrombin (AT) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCC313Hu | CLIA Kit for Antithrombin (AT) | Chemiluminescent immunoassay for Antigen Detection. |
LMC313Hu | Multiplex Assay Kit for Antithrombin (AT) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
KSC313Hu01 | ELISA Kit DIY Materials for Antithrombin (AT) | Main materials for "Do It (ELISA Kit) Yourself". |