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Recombinant Histone H4 (H4)

  • Product No.RPA289Mi01
  • Organism SpeciesMus musculus (Mouse) Same name, Different species.
  • SourceProkaryotic expression
  • HostE.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method).
  • Subcellular LocationSecreted
  • Predicted Molecular Mass41.3kDa
  • Accurate Molecular Mass44kDa(Analysis of differences refer to the manual)
  • Residues & TagsSer2~Gly103 with N-terminal His and GST Tag
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
  • Traits Freeze-dried powder
  • Purity> 80%
  • Isoelectric Point12.8
  • Applications Positive Control; Immunogen; SDS-PAGE; WB.
    If bio-activity of the protein is needed, please check active protein.
  • DownloadInstruction Manual
  • UOM 10µg50µg 200µg 1mg 5mg
  • FOB US$ 164  US$ 410  US$ 820  US$ 2460  US$ 6150 
    For more details, please contact local distributors!

SEQUENCE

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
PLoS One. N-Acetyl-Heparin Attenuates Acute Lung Injury Caused by Acid Aspiration Mainly by Antagonizing Histones in Mice Pubmed:24816808
Crit Care. Impact of plasma histones in human sepsis and their contribution to cellular injury and inflammation Pubmed:25260379
Biomed Res Int A Novel Anti-Histone H2 Monoclonal Antibody, SSV Monoclonal Antibody, Improves Lung Injury and Survival in a Mouse Model of Lipopolysaccharide-Induced Sepsis-Like Syndrome PubMed: 25649891
Journal of Viral Hepatitis Patients with HBV-related acute-on-chronic liver failure have increased concentrations of extracellular histones aggravating cellular damage and systemic inflammation pubmed:27660136
Cell Death & Disease Dexmedetomidine inhibits astrocyte pyroptosis and subsequently protects the brain in in vitro and in vivo models of sepsis Pubmed: 30778043
Respir Res Histone H4 induces heparan sulfate degradation by activating heparanase in chlorine gas-induced acute respiratory distress syndrome Pubmed:35073921
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPA289Mi02 Recombinant Histone H4 (H4) Positive Control; Immunogen; SDS-PAGE; WB.
RPA289Mi01 Recombinant Histone H4 (H4) Positive Control; Immunogen; SDS-PAGE; WB.
PAA289Mi02 Polyclonal Antibody to Histone H4 (H4) WB; IHC; ICC; IP.
PAA289Mi01 Polyclonal Antibody to Histone H4 (H4) WB; IHC; ICC; IP.
SEA289Mi ELISA Kit for Histone H4 (H4) Enzyme-linked immunosorbent assay for Antigen Detection.
HEA289Mi High Sensitive ELISA Kit for Histone H4 (H4) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA289Mi Multiplex Assay Kit for Histone H4 (H4) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.