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ELISA Kit for Histone H4 (H4)
- Product No.SEA289Mi
- Organism SpeciesHomo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat) Same name, Different species.
- Sample Typetissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range0.156-10ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.059ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
- FOB
US$ 454
For more details, please contact local distributors! US$ 648 US$ 2916 US$ 5508 US$ 45360
Specificity
This assay has high sensitivity for detection of Histone H4 (H4).
100% cross-reactivity of Histone H4 (H4) was observed among Human, Mouse, Rat.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Histone H4 (H4) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Histone H4 (H4) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
Standard | 2 | Standard Diluent | 1×20mL |
Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
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Magazine | Citations |
PLoS One. | N-Acetyl-Heparin Attenuates Acute Lung Injury Caused by Acid Aspiration Mainly by Antagonizing Histones in Mice Pubmed:24816808 |
Crit Care. | Impact of plasma histones in human sepsis and their contribution to cellular injury and inflammation Pubmed:25260379 |
Biomed Res Int | A Novel Anti-Histone H2 Monoclonal Antibody, SSV Monoclonal Antibody, Improves Lung Injury and Survival in a Mouse Model of Lipopolysaccharide-Induced Sepsis-Like Syndrome PubMed: 25649891 |
Journal of Viral Hepatitis | Patients with HBV-related acute-on-chronic liver failure have increased concentrations of extracellular histones aggravating cellular damage and systemic inflammation pubmed:27660136 |
Cell Death & Disease | Dexmedetomidine inhibits astrocyte pyroptosis and subsequently protects the brain in in vitro and in vivo models of sepsis Pubmed: 30778043 |
Respir Res | Histone H4 induces heparan sulfate degradation by activating heparanase in chlorine gas-induced acute respiratory distress syndrome Pubmed:35073921 |
Catalog No. | Related products for research use of Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
RPA289Mi02 | Recombinant Histone H4 (H4) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA289Mi01 | Recombinant Histone H4 (H4) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA289Mi02 | Polyclonal Antibody to Histone H4 (H4) | WB; IHC; ICC; IP. |
PAA289Mi01 | Polyclonal Antibody to Histone H4 (H4) | WB; IHC; ICC; IP. |
SEA289Mi | ELISA Kit for Histone H4 (H4) | Enzyme-linked immunosorbent assay for Antigen Detection. |
HEA289Mi | High Sensitive ELISA Kit for Histone H4 (H4) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA289Mi | Multiplex Assay Kit for Histone H4 (H4) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |