Packages (Simulation)
Image (I)
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DAB staining on IHC-P; Samples: Mouse Kidney Tissue; Primary Ab: 20μg/ml Rabbit Anti-Mouse GLS Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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Western Blot; Sample: 293T cell lysate
Primary Ab: 0.2µg/ml Rabbit Anti-Mouse GLS Antibody
Second Ab: 0.2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Mouse Kidney Tissue
Primary Ab: 20µg/ml Rabbit Anti-Mouse GLS Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Mouse Skeletal muscle Tissue
Primary Ab: 20µg/ml Rabbit Anti-Mouse GLS Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Image (II)
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Figure. Western Blot; Sample: Recombinant GLS, Mouse.
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Knockout Varification:
Lane 1: Wild-type 293T cell lysate;
Lane 2: GLS knockout 293T cell lysate;
Predicted MW: 73,65kd
Observed MW: 60kd
Primary Ab: 2µg/ml Rabbit Anti-Mouse GLS Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Mouse Cerebrum lysate;
Primary Ab: 2µg/ml Rabbit Anti-Mouse GLS Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Mouse Kidney lysate;
Primary Ab: 2µg/ml Rabbit Anti-Mouse GLS Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Quality Guarantee
Instruction Manual
Certificate
- KO-Validated
Polyclonal Antibody to Glutaminase (GLS)
GLS1; Glutaminase kidney isoform, mitochondrial; K-glutaminase; L-glutamine amidohydrolase
- Product No.PAJ026Mu01
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPJ026Mu01-Recombinant Glutaminase (GLS)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration0.5mg/mL
- Organism Species MoreHomo sapiens (Human)
- ApplicationsWB; IHC
If the antibody is used in flow cytometry, please check FCM antibodies. - DownloadInstruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
- FOB
US$ 108
US$ 252
US$ 360
US$ 900
US$ 3600
For more details, please contact local distributors!
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against GLS. It has been selected for its ability to recognize GLS in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
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Antibody Labeling Customized Service
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Protein A/G Purification Column
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Staining Solution for Cells and Tissue
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Western Blot (WB) Experiment Service
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Immunohistochemistry (IHC) Experiment Service
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Immunocytochemistry (ICC) Experiment Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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Buffer
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DAB Chromogen Kit
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SABC Kit
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Long-arm Biotin Labeling Kit
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Real Time PCR Experimental Service
| Magazine | Citations |
| Journal of Neuroscience | Sex differences in the glutamate signaling pathway in juvenile rats Pubmed:28861894 |
| Journal of Affective Disorders | Glutamatergic hypofunction in medication-free major depression: Secondary effects of affective diagnosis and relationship to peripheral glutaminase Pubmed:29544167 |
| 校刊 | King's Research Portal Doi: 10.1016/j.jad.2018.02.059 |
| bmc research notes | The role of CA-125, GLS and FASN in predicting cytoreduction for epithelial ovarian cancers Pubmed: 32698888 |
| Catalog No. | Related products for research use of Mus musculus (Mouse) Organism species | Applications (RESEARCH USE ONLY!) |
| RPJ026Mu01 | Recombinant Glutaminase (GLS) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPJ026Mu02 | Recombinant Glutaminase (GLS) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAJ026Mu02 | Polyclonal Antibody to Glutaminase (GLS) | WB; IHC; ICC; IP. |
| PAJ026Mu01 | Polyclonal Antibody to Glutaminase (GLS) | WB; IHC |
| LAJ026Mu41 | PE-Linked Polyclonal Antibody to Glutaminase (GLS) | WB |
| SEJ026Mu | ELISA Kit for Glutaminase (GLS) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMJ026Mu | Multiplex Assay Kit for Glutaminase (GLS) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
| CMJ026Mu | Multiplex Assay Kit for Glutaminase (GLS) ,etc. by CBA (Cytometric Bead Array) | FLIA Kit for Antigen Detection. |