Packages (Simulation)

Polyclonal Antibody to Sirtuin 7 (SIRT7)

Image (I)

FITC staining on IF; Samples: Human HepG2 Cells; Primary Ab: 10µg/ml Rabbit Anti-Rat SIRT7 Antibody Second Ab: 1µg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb18)

Image (II)

WB Image
Western Blot; Sample: Rat Lung lysate;
Primary Ab: 2µg/ml Rabbit Anti-Rat SIRT7 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Rat Liver lysate;
Primary Ab: 2µg/ml Rabbit Anti-Rat SIRT7 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

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Instruction Manual

Polyclonal-Antibody-to-Sirtuin-7-(SIRT7)-PAE917Ra01.pdf

Certificate

Polyclonal Antibody to Sirtuin 7 (SIRT7)

SIR2L7; NAD-Dependent Deacetylase Sirtuin-7; Silent Mating Type Information Regulation 2 Homolog 7; Regulatory protein SIR2 homolog 7; SIR2-like protein 7

Product No.PAE917Ra01
Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPE917Ra01-Recombinant Sirtuin 7 (SIRT7)
Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
TraitsLiquid
Concentration0.44mg/mL
Organism Species MoreMus musculus (Mouse)
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 114 266 US$ 266 US$ 380 US$ 950 US$ 3800
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against SIRT7. It has been selected for its ability to recognize SIRT7 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine	Citations
Elife	SIRT2 deacetylase regulates the activity of GSK3 isoforms independent of inhibitory phosphorylation Pubmed:29504933

Catalog No.	Related products for research use of Rattus norvegicus (Rat) Organism species	Applications (RESEARCH USE ONLY!)
RPE917Ra01	Recombinant Sirtuin 7 (SIRT7)	Positive Control; Immunogen; SDS-PAGE; WB.
RPE917Ra02	Recombinant Sirtuin 7 (SIRT7)	Positive Control; Immunogen; SDS-PAGE; WB.
PAE917Ra01	Polyclonal Antibody to Sirtuin 7 (SIRT7)	WB; IHC; ICC; IP.
PAE917Ra02	Polyclonal Antibody to Sirtuin 7 (SIRT7)	WB; IHC; ICC; IP.
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SEE917Ra	ELISA Kit for Sirtuin 7 (SIRT7)	Enzyme-linked immunosorbent assay for Antigen Detection.
LME917Ra	Multiplex Assay Kit for Sirtuin 7 (SIRT7) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.
KSE917Ra01	ELISA Kit DIY Materials for Sirtuin 7 (SIRT7)	Main materials for "Do It (ELISA Kit) Yourself".