Packages (Simulation)

Polyclonal Antibody to Sirtuin 4 (SIRT4)

Image (I)

DAB staining on IHC-P; Samples: Mouse Testis Tissue; Primary Ab: 10µg/ml Rabbit Anti-Mouse SIRT4 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Mouse Skeletal muscle Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Mouse SIRT4 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

Image (II)

Figure. Western Blot; Sample: Recombinant SIRT4, Mouse.
Figure. Western Blot; Sample: Lane1: Mouse Liver Tissue; Lane2: Human Hela Cells; Lane3: Human PC-3 Cells.

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Instruction Manual

Polyclonal-Antibody-to-Sirtuin-4-(SIRT4)-PAE914Mu01.pdf

Certificate

Polyclonal Antibody to Sirtuin 4 (SIRT4)

SIR2L4; Silent Mating Type Information Regulation 2 Homolog 4; SIR2-like protein 4; NAD-dependent protein deacetylase sirtuin-4

Product No.PAE914Mu01
Organism SpeciesMus musculus (Mouse) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPE914Mu01-Recombinant Sirtuin 4 (SIRT4)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid
Concentration500µg/mL
Organism Species MoreHomo sapiens (Human)
ApplicationsWB; IHC
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 108 252 US$ 252 US$ 360 US$ 900 US$ 3600
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against SIRT4. It has been selected for its ability to recognize SIRT4 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.5-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine	Citations
Growth	Preliminary data on the relationship between circulating levels of Sirtuin 4, anthropometric and metabolic parameters in obese subjects according to growth hormone/insulin-like growth factor-1 status PubMed: 25466907
hindawi	ADHERENCE TO THE MEDITERRANEAN DIET AND CIRCULATING LEVELS OF SIRTUIN 4 IN OBESE 6101254.pdf
Oxidative Medicine and Cellular Longevity	Adherence to the Mediterranean Diet and Circulating Levels of Sirtuin 4 in Obese Patients: A Novel Association pubmed:28706576
Elife	SIRT2 deacetylase regulates the activity of GSK3 isoforms independent of inhibitory phosphorylation Pubmed:29504933

Catalog No.	Related products for research use of Mus musculus (Mouse) Organism species	Applications (RESEARCH USE ONLY!)
RPE914Mu01	Recombinant Sirtuin 4 (SIRT4)	Positive Control; Immunogen; SDS-PAGE; WB.
PAE914Mu01	Polyclonal Antibody to Sirtuin 4 (SIRT4)	WB; IHC
LAE914Mu71	Biotin-Linked Polyclonal Antibody to Sirtuin 4 (SIRT4)	WB
KSE914Mu01	ELISA Kit DIY Materials for Sirtuin 4 (SIRT4)	Main materials for "Do It(ELISA Kit) Yourself"