Packages (Simulation)
Image (I)
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FITC staining on IF; Sample: Human HepG2 cell; Primary Ab: 10µg/ml Rabbit Anti-Human HK2 Antibody Second Ab: 2µg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb18)
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Figure: FITC staining on IHC-P;
Sample: MCF7 cells.
Image (II)
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WB Image
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Western Blot; Sample: Human Jurkat cell lysate;
Primary Ab: 5µg/ml Rabbit Anti-Human HK2 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Knockout Varification:
Lane 1: Wild-type MCF7 cell lysate;
Lane 2: HK2 knockout MCF7 cell lysate;
Predicted MW: 102kd
Observed MW: 102kd
Primary Ab: 5µg/ml Rabbit Anti-Human HK2 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Human MCF7 cell lysate;
Primary Ab: 5µg/ml Rabbit Anti-Human HK2 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Human A431 cell lysate;
Primary Ab: 5µg/ml Rabbit Anti-Human HK2 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Human Hela cell lysate;
Primary Ab: 5µg/ml Rabbit Anti-Human HK2 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Human HepG2 cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-human HK2 Polyclonal Antibody (Catalog PAD352Hu02, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
Quality Guarantee
Instruction Manual
Certificate
- KO-Validated
Polyclonal Antibody to Hexokinase 2 (HK2)
HK II; Muscle form hexokinase
- Product No.PAD352Hu02
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPD352Hu02-Recombinant Hexokinase 2 (HK2)
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid
- Concentration0.39mg/mL
- Organism Species MoreMus musculus (Mouse)
- ApplicationsWB; IHC; ICC; IP; FCM
If the antibody is used in flow cytometry, please check FCM antibodies. - DownloadInstruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
- FOB
US$ 105
US$ 245
US$ 350
US$ 875
US$ 3500
For more details, please contact local distributors!
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against HK2. It has been selected for its ability to recognize HK2 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.01-5μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Flow cytometry:20μg/mL;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
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Antibody Labeling Customized Service
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Protein A/G Purification Column
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Staining Solution for Cells and Tissue
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Western Blot (WB) Experiment Service
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Immunohistochemistry (IHC) Experiment Service
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Immunocytochemistry (ICC) Experiment Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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Buffer
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DAB Chromogen Kit
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SABC Kit
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Long-arm Biotin Labeling Kit
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Real Time PCR Experimental Service
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| Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
| RPD352Hu01 | Recombinant Hexokinase 2 (HK2) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPD352Hu02 | Recombinant Hexokinase 2 (HK2) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAD352Hu01 | Polyclonal Antibody to Hexokinase 2 (HK2) | WB; IHC; ICC; IP. |
| PAD352Hu02 | Polyclonal Antibody to Hexokinase 2 (HK2) | WB; IHC; ICC; IP; FCM |
| MAD352Hu21 | Monoclonal Antibody to Hexokinase 2 (HK2) | WB; IHC; ICC; IP. |
| SED352Hu | ELISA Kit for Hexokinase 2 (HK2) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMD352Hu | Multiplex Assay Kit for Hexokinase 2 (HK2) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |