Packages (Simulation)

Polyclonal Antibody to Collagen Type IV Alpha 3 (COL4a3)

Image (I)

DAB staining on IHC-P; Samples: Human Kidney Tissue; Primary Ab: 10µg/ml Rabbit Anti-Human COL4a3 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Stomach Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human COL4a3 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

Image (II)

Figure. Western Blot; Sample: Recombinant COL4a3, Human.
Western Blot; Sample: Mouse Skeletal muscle lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human COL4a3 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

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Instruction Manual

Polyclonal-Antibody-to-Collagen-Type-IV-Alpha-3-(COL4a3)-PAC143Hu01.pdf

Certificate

Polyclonal Antibody to Collagen Type IV Alpha 3 (COL4a3)

COL4-A3; Tumstatin; Goodpasture Antigen; Collagen Alpha-3(IV)chain

Product No.PAC143Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPC143Hu01-Recombinant Collagen Type IV Alpha 3 (COL4a3)
Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
TraitsLiquid
Concentration0.5mg/mL
Organism Species MoreMus musculus (Mouse)
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 105 245 US$ 245 US$ 350 US$ 875 US$ 3500
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against COL4a3. It has been selected for its ability to recognize COL4a3 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine	Citations
Experimental and Molecular Pathology	Epitope recognized by anti-glomerular basement membrane (GBM) antibody in a patient with repeated relapse of anti-GBM disease Pubmed: 30817909

Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
RPC143Hu02	Recombinant Collagen Type IV Alpha 3 (COL4a3)	Positive Control; Immunogen; SDS-PAGE; WB.
RPC143Hu01	Recombinant Collagen Type IV Alpha 3 (COL4a3)	Positive Control; Immunogen; SDS-PAGE; WB.
PAC143Hu01	Polyclonal Antibody to Collagen Type IV Alpha 3 (COL4a3)	WB; IHC; ICC; IP.
MAC143Hu22	Monoclonal Antibody to Collagen Type IV Alpha 3 (COL4a3)	WB; IHC; ICC; IP.
MAC143Hu21	Monoclonal Antibody to Collagen Type IV Alpha 3 (COL4a3)	WB; IHC; ICC; IP.
MAC143Hu23	Monoclonal Antibody to Collagen Type IV Alpha 3 (COL4a3)	WB; IHC; ICC; IP.
SEC143Hu	ELISA Kit for Collagen Type IV Alpha 3 (COL4a3)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMC143Hu	Multiplex Assay Kit for Collagen Type IV Alpha 3 (COL4a3) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.