Packages (Simulation)

Polyclonal Antibody to Interferon Regulatory Factor 3 (IRF3)

Image (I)

Used in DAB staining on fromalin fixed paraffin-embedded Kidney tissue
Figure: FITC staining on IHC-P;
Sample: Hela cells.
AF488 staining on IF;
Sample: Hepa1-6 cell
Primary Ab: 20µg/ml Rabbit Anti-Human IRF3 Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)
AF488 staining on IF;
Sample: NIH/3T3 cell
Primary Ab: 20µg/ml Rabbit Anti-Human IRF3 Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)

Image (II)

Western Blot; Sample: Lane1: 3T3-L1 cell lysate; Lane2: Rat Kidney lysate Primary Ab: 0.6µg/ml Rabbit Anti-Human IRF3 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
Western Blot; Sample: Human Jurkat cell lysate;
Primary Ab: 3µg/ml Rabbit Anti-Human IRF3 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Hela cell lysate
Primary Ab: 0.3µg/ml Rabbit Anti-Human IRF3 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Human Hela cell line was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-human IRF3 Polyclonal Antibody (Catalog PAB589Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).

Quality Guarantee

Instruction Manual

Polyclonal-Antibody-to-Interferon-Regulatory-Factor-3-(IRF3)-PAB589Hu01.pdf

Certificate

Polyclonal Antibody to Interferon Regulatory Factor 3 (IRF3)

Product No.PAB589Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPB589Hu01-Recombinant Interferon Regulatory Factor 3 (IRF3)
Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
TraitsLiquid
Concentration0.5mg/ml
Organism Species MoreMus musculus (Mouse), Rattus norvegicus (Rat)
ApplicationsWB; IHC; ICC; IP; FCM
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 100 232 US$ 232 US$ 332 US$ 830 US$ 3320
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against IRF3. It has been selected for its ability to recognize IRF3 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-3μg/mL
Immunohistochemistry: 5-30μg/mL
Immunocytochemistry: 5-30μg/mL
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Magazine	Citations
PLOS Pathogens	RIG-I-like receptor activation by dengue virus drives follicular T helper cell formation and antibody production pubmed:29186193

Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
RPB589Hu01	Recombinant Interferon Regulatory Factor 3 (IRF3)	Positive Control; Immunogen; SDS-PAGE; WB.
PAB589Hu01	Polyclonal Antibody to Interferon Regulatory Factor 3 (IRF3)	WB; IHC; ICC; IP; FCM
MAB589Hu22	Monoclonal Antibody to Interferon Regulatory Factor 3 (IRF3)	WB; IHC; ICC; IP.
SEB589Hu	ELISA Kit for Interferon Regulatory Factor 3 (IRF3)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMB589Hu	Multiplex Assay Kit for Interferon Regulatory Factor 3 (IRF3) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.