Packages (Simulation)

Polyclonal Antibody to Cluster Of Differentiation 99 (CD99)

Image (I)

Used in DAB staining on fromalin fixed paraffin- embedded Kidney tissue

Image (II)

Figure. Western Blot; Sample: Recombinant CD99, Rat.
Western Blot; Sample: Rat Lung lysate
Primary Ab: 0.006µg/ml Rabbit Anti-Rat CD99 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Rat Thymus lysate;
Primary Ab: 1µg/ml Rabbit Anti-Rat CD99 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Lane1: Rat Spleen lysate; Lane2: Rat Testis lysate
Primary Ab: 0.01µg/ml Rabbit Anti-Rat CD99 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

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Instruction Manual

Polyclonal-Antibody-to-Cluster-Of-Differentiation-99-(CD99)-PAB426Ra01.pdf

Certificate

Polyclonal Antibody to Cluster Of Differentiation 99 (CD99)

MIC2X; MIC2Y; MIC2; Antigen Identified By Monoclonal Antibodies 12E7,F21 And O13; E2 antigen; T-cell surface glycoprotein E2

Product No.PAB426Ra01
Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPB426Ra01-Recombinant Cluster Of Differentiation 99 (CD99)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid
Concentration500µg/mL
Organism Species Moren/a
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 108 252 US$ 252 US$ 360 US$ 900 US$ 3600
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against CD99. It has been selected for its ability to recognize CD99 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Catalog No.	Related products for research use of Rattus norvegicus (Rat) Organism species	Applications (RESEARCH USE ONLY!)
RPB426Ra01	Recombinant Cluster Of Differentiation 99 (CD99)	Positive Control; Immunogen; SDS-PAGE; WB.
RPB426Ra02	Recombinant Cluster Of Differentiation 99 (CD99)	Positive Control; Immunogen; SDS-PAGE; WB.
PAB426Ra01	Polyclonal Antibody to Cluster Of Differentiation 99 (CD99)	WB; IHC; ICC; IP.