Packages (Simulation)

Polyclonal Antibody to Myosin Light Chain Kinase (MYLK)

Image (I)

DAB staining on IHC-P; Samples: Human Esophagus cancer Tissue; Primary Ab: 20µg/ml Rabbit Anti-Human MYLK Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Kidney Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human MYLK Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Rat Colon Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human MYLK Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Rat Uterus Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human MYLK Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Rat Small intestine Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human MYLK Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Rat Cardiac Muscle Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human MYLK Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

Image (II)

Figure. Western Blot; Sample: Recombinant MYLK, Human.
Western Blot; Samples: Lane1: Human Lung lysate; Lane2: Rat Bladder lysate; Lane3: Rat Prostate lysate;
Primary Ab: 2µg/ml Rabbit Anti-Human MYLK Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Samples: Lane1: Hela cell lysate; Lane2: HepG2 cell lysate;
Primary Ab: 0.2µg/ml Rabbit Anti-Human MYLK Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

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Instruction Manual

Polyclonal-Antibody-to-Myosin-Light-Chain-Kinase-(MYLK)-PAB106Hu01.pdf

Certificate

Polyclonal Antibody to Myosin Light Chain Kinase (MYLK)

KRP; MLCK108; MLCK210; MSTP083; MLCK; smMLCK; MYLK1; MLCK1; Telokin; Myosin Light Chain Kinase, Smooth Muscle, deglutamylated form; Kinase-related protein

Product No.PAB106Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPB106Hu01-Recombinant Myosin Light Chain Kinase (MYLK)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid
Concentration0.5mg/ml
Organism Species MoreRattus norvegicus (Rat)
ApplicationsWB; IHC
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 100 232 US$ 232 US$ 332 US$ 830 US$ 3320
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against MYLK. It has been selected for its ability to recognize MYLK in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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INCREMENT SERVICES

Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
RPB106Hu01	Recombinant Myosin Light Chain Kinase (MYLK)	Positive Control; Immunogen; SDS-PAGE; WB.
PAB106Hu01	Polyclonal Antibody to Myosin Light Chain Kinase (MYLK)	WB; IHC
MAB106Hu22	Monoclonal Antibody to Myosin Light Chain Kinase (MYLK)	WB; IHC; ICC; IP.
SEB106Hu	ELISA Kit for Myosin Light Chain Kinase (MYLK)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMB106Hu	Multiplex Assay Kit for Myosin Light Chain Kinase (MYLK) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.