Packages (Simulation)

Polyclonal Antibody to Apoptosis Inducing Factor (AIF)

Image (I)

DAB staining on IHC-P; Samples: Human Liver Tissue; Primary Ab: 10µg/ml Rabbit Anti-Human AIF Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Stomach Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human AIF Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Secondary antibody only control: Used PBS instead of primary antibody, Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Kidney Tissue;
Primary Ab: 30µg/ml Rabbit Anti-Human AIF Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Secondary antibody only control: Used PBS instead of primary antibody, Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
FITC staining on IF;
Sample: Human Hela cell;
Primary Ab: 20ug/ml Rabbit Anti-Human AIF Antibody
Second Ab: 2µg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb18)

Image (II)

Figure. Western Blot; Sample: Recombinant AIF, Human.
Western Blot; Sample: Lane1: A549 cell lysate; Lane2: Porcine Heart lysate
Primary Ab: 0.3μg/ml Rabbit Anti-Human AIF Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Knockout Varification:
Lane 1: Wild-type A549 cell lysate;
Lane 2: AIF knockout A549 cell lysate;
Predicted MW: 26~35,66kd
Observed MW: 66kd
Primary Ab: 3µg/ml Rabbit Anti-Human AIF Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Lane1: Hela cell lysate; Lane2: Bovine Heart lysate
Primary Ab: 0.3μg/ml Rabbit Anti-Human AIF Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Samples: Lane1: Rat Liver lysate; Lane2: Rat Kidney lysate; Lane3: Rat Heart lysate;
Primary Ab: 0.3µg/ml Rabbit Anti-Human AIF Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

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Instruction Manual

Polyclonal-Antibody-to-Apoptosis-Inducing-Factor-(AIF)-PAB064Hu01.pdf

Certificate

KO-Validated

Polyclonal Antibody to Apoptosis Inducing Factor (AIF)

PDCD8; Programmed Cell Death protein 8; Apoptosis-inducing factor 1, mitochondrial

Product No.PAB064Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPB064Hu01-Recombinant Apoptosis Inducing Factor (AIF)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid
Concentration0.5mg/mL
Organism Species MoreSus scrofa; Porcine (Pig), Bos taurus; Bovine (Cattle)
ApplicationsWB,IHC,ICC/IF
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 100 232 US$ 232 US$ 332 US$ 830 US$ 3320
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against AIF. It has been selected for its ability to recognize AIF in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-3µg/mL;
Immunohistochemistry: 5-30µg/mL;
Immunocytochemistry: 5-30µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine	Citations
	РОЛЬ АПОПТОЗ ИНДУЦИРУЮЩЕГО ФАКТОРА И ОКИСЛИТЕЛЬНОГО СТРЕССА ПРИ ХРОНИЧЕСКОЙ СЕРДЕЧНОЙ НЕДОСТАТОЧНОСТИ 616-005.8
NEUROCHEMISTRY INTERNATIONAL	Redox-active DJ-1 sustains brainstem cardiovascular regulation via maintenance of mitochondrial function during mevinphos intoxication Pubmed: 32650030
Iranian Journal of Basic Medical Sciences	Chromon-3-aldehyde derivatives restore mitochondrial function in rat cerebral ischemia Pubmed: 32963739
Journal of?Pharmaceutical Sciences	Correction of mitochondrial dysfunction by 4-hydroxy-3, 5-ditretbutyl cinnamic acid in experimental Alzheimer's disease induced by A¦Â injection in rats.

Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
RPB064Hu01	Recombinant Apoptosis Inducing Factor (AIF)	Positive Control; Immunogen; SDS-PAGE; WB.
PAB064Hu01	Polyclonal Antibody to Apoptosis Inducing Factor (AIF)	WB,IHC,ICC/IF
MAB064Hu22	Monoclonal Antibody to Apoptosis Inducing Factor (AIF)	WB; IHC; ICC; IP.
SEB064Hu	ELISA Kit for Apoptosis Inducing Factor (AIF)	Enzyme-linked immunosorbent assay for Antigen Detection.
SCB064Hu	CLIA Kit for Apoptosis Inducing Factor (AIF)	Chemiluminescent immunoassay for Antigen Detection.
LMB064Hu	Multiplex Assay Kit for Apoptosis Inducing Factor (AIF) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.