Packages (Simulation)

Polyclonal Antibody to Apoptotic Peptidase Activating Factor 1 (APAF1)

Image (I)

DAB staining on IHC-P; Samples: Human Liver Tissue; Primary Ab: 10µg/ml Rabbit Anti-Human APAF1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Lymphoma Tissue;
Primary Ab: 30ug/ml Rabbit Anti-Human APAF1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Colorectal cancer Tissue;
Primary Ab: 30ug/ml Rabbit Anti-Human APAF1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
FITC staining on IF;
Samples: Human Hela cell;
Primary Ab: 20μg/ml Rabbit Anti-Human APAF1 Antibody
Second Ab: 1.5μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb18)

Image (II)

Figure. Western Blot; Sample: Recombinant APAF1, Human.
Western Blot; Sample: Lane1: THP1 cell lysate; Lane2: 293T cell lysate
Primary Ab: 1.5μg/ml Rabbit Anti-Human APAF1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

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Instruction Manual

Polyclonal-Antibody-to-Apoptotic-Peptidase-Activating-Factor-1-(APAF1)-PAB054Hu01.pdf

Certificate

Polyclonal Antibody to Apoptotic Peptidase Activating Factor 1 (APAF1)

CED4; Apoptotic Protease Activating Factor

Product No.PAB054Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPB054Hu01-Recombinant Apoptotic Peptidase Activating Factor 1 (APAF1)
Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
TraitsLiquid
Concentration0.5mg/ml
Organism Species Moren/a
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 100 232 US$ 232 US$ 332 US$ 830 US$ 3320
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against APAF1. It has been selected for its ability to recognize APAF1 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-3µg/mL;
Immunohistochemistry: 5-30µg/mL;
Immunocytochemistry: 5-30µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine	Citations
Iranian Journal of phamaceutical research	Potential anti-tumor activity of kefir-induced juglone and resveratrol fractions against Ehrlich ascites carcinoma-bearing BALB/c mice

Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
RPB054Hu01	Recombinant Apoptotic Peptidase Activating Factor 1 (APAF1)	Positive Control; Immunogen; SDS-PAGE; WB.
PAB054Hu01	Polyclonal Antibody to Apoptotic Peptidase Activating Factor 1 (APAF1)	WB; IHC; ICC; IP.
MAB054Hu22	Monoclonal Antibody to Apoptotic Peptidase Activating Factor 1 (APAF1)	WB; IHC; ICC; IP.
SEB054Hu	ELISA Kit for Apoptotic Peptidase Activating Factor 1 (APAF1)	Enzyme-linked immunosorbent assay for Antigen Detection.
SCB054Hu	CLIA Kit for Apoptotic Peptidase Activating Factor 1 (APAF1)	Chemiluminescent immunoassay for Antigen Detection.
LMB054Hu	Multiplex Assay Kit for Apoptotic Peptidase Activating Factor 1 (APAF1) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.