Polyclonal Antibody to Cytochrome P450 7A1 (CYP7A1)

CYP7; CYP7-A1; CYPVII; Cholesterol 7 Alpha Hydroxylase; Cytochrome P450 Family 7 Subfamily A Polypeptide 1

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against CYP7A1. It has been selected for its ability to recognize CYP7A1 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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INCREMENT SERVICES

Magazine Citations
Cell Reports Parabacteroides distasonis Alleviates Obesity and Metabolic Dysfunctions via Production of Succinate and Secondary Bile Acids Pubmed: 30605678
Journal of Functional Foods Hyperglycemia exacerbates dyslipidemia-induced changes in uptake, synthesis, and transporters of bile acids in rats: Assessment of restorative potentials of ALA and?… Doi: 10.1016/j.jff.2019.01.038
Journal of Agriculture and Food Research Withaferin-A down-regulate enterohepatic circulation of bile acids: An insight from a hyperlipidemic rat model
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