Packages (Simulation)

Polyclonal Antibody to Retinoic Acid Receptor Alpha (RARa)

Image (I)

IHC Images
AF488 staining on IF;
Sample: HepG2 cell
Primary Ab: 20µg/ml Rabbit Anti-Mouse RARa Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)
AF488 staining on IF;
Sample: MCF7 cell
Primary Ab: 20µg/ml Rabbit Anti-Mouse RARa Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)

Image (II)

Figure. Western Blot; Sample: Recombinant RARa, Mouse.
Figure. Western Blot; Sample: Lane1: Human A431 Cells; Lane2: Human HepG2 Cells.
HepG2 human hepatocellular carcinoma cell line was stained with rabbit Anti-mouse RARa Polyclonal Antibody (Catalog # PAA976Mu01, filled red histogram) or Isotype control antibody (Catalog # IS067-Rb01, filled green histogram), followed by APC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog # SAA544Rb15). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.

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Instruction Manual

Polyclonal-Antibody-to-Retinoic-Acid-Receptor-Alpha-(RARa)-PAA976Mu01.pdf

Certificate

Polyclonal Antibody to Retinoic Acid Receptor Alpha (RARa)

RAR-A; NR1B1; RAR; RAR-Alpha; Nuclear Receptor Subfamily 1 Group B Member 1

Product No.PAA976Mu01
Organism SpeciesMus musculus (Mouse) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPA976Mu01-Recombinant Retinoic Acid Receptor Alpha (RARa)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid
Concentration500µg/mL
Organism Species MoreHomo sapiens (Human), Mus musculus (Mouse)
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 97 227 US$ 227 US$ 324 US$ 810 US$ 3240
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against RARa. It has been selected for its ability to recognize RARa in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine	Citations
Nature Communications	Retinoic acid receptor alpha is associated with tamoxifen resistance in breast cancer PubMed: PMC3759040
COPD: Journal of Chronic Obstructive Pulmonary Disease	Retinoic acid receptor alpha: One of plasma biomarkers associated with exacerbation of chronic obstructive pulmonary disease Pubmed: 24111541

Catalog No.	Related products for research use of Mus musculus (Mouse) Organism species	Applications (RESEARCH USE ONLY!)
RPA976Mu01	Recombinant Retinoic Acid Receptor Alpha (RARa)	Positive Control; Immunogen; SDS-PAGE; WB.
PAA976Mu01	Polyclonal Antibody to Retinoic Acid Receptor Alpha (RARa)	WB; IHC; ICC; IP.
FAA976Mu01	Anti-Retinoic Acid Receptor Alpha (RARa) Polyclonal Antibody	Flow cytometry.