Packages (Simulation)
Image (I)
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Used in DAB staining on fromalin fixed paraffin- embedded skin tissue
Image (II)
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WB Image
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Western Blot; Sample: Rat Skin lysate;
Primary Ab: 2µg/ml Rabbit Anti-Rat CASP14 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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Instruction Manual
Certificate
Polyclonal Antibody to Caspase 14 (CASP14)
Caspase 14 Apoptosis-Related Cysteine Peptidase; Cysteinyl Aspartate Specific Proteinases 14
- Product No.PAA457Ra01
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen n/a
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid
- Concentration500µg/mL
- Organism Species Moren/a
- ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies. - DownloadInstruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
- FOB
US$ 103
US$ 239
US$ 342
US$ 855
US$ 3420
For more details, please contact local distributors!
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against CASP14. It has been selected for its ability to recognize CASP14 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
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Antibody Labeling Customized Service
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Protein A/G Purification Column
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Staining Solution for Cells and Tissue
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Western Blot (WB) Experiment Service
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Immunohistochemistry (IHC) Experiment Service
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Immunocytochemistry (ICC) Experiment Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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Buffer
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DAB Chromogen Kit
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SABC Kit
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Long-arm Biotin Labeling Kit
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Real Time PCR Experimental Service
| Magazine | Citations |
| Journal of Dermatological Science | Pyrrolidone carboxylic acid levels or caspase-14 expression in the corneocytes of lesional skin correlates with clinical severity, skin barrier function and lesional inflammation in atopic dermatitis Pubmed:25315296 |
| In Vitro Cell Dev Biol Anim | Luteolin induces caspase-14-mediated terminal differentiation in human epidermal keratinocytes PubMed: 26427706 |
| Applied Biochemistry and Biotechnology | Enhanced Induction of Apoptosis in HaCaT Cells by Luteolin Encapsulated in PEGylated Liposomes—Role of Caspase-3/Caspase-14 Pubmed: 30390204 |
| Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
| RPA457Ra01 | Recombinant Caspase 14 (CASP14) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA457Ra01 | Polyclonal Antibody to Caspase 14 (CASP14) | WB; IHC; ICC; IP. |
| MAA457Ra21 | Monoclonal Antibody to Caspase 14 (CASP14) | WB; IHC; ICC; IP. |
| SEA457Ra | ELISA Kit for Caspase 14 (CASP14) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA457Ra | Multiplex Assay Kit for Caspase 14 (CASP14) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |