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Multiplex Assay Kit for Caspase 14 (CASP14) ,etc. by FLIA (Flow Luminescence Immunoassay)
Caspase 14 Apoptosis-Related Cysteine Peptidase; Cysteinyl Aspartate Specific Proteinases 14
(Note: Up to 8-plex in one testing reaction)
- Product No.LMA457Ra
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Sample TypeTissue homogenates, cell lysates and other biological fluids.
- Test MethodDouble-antibody Sandwich
- Assay Length3.5h
- Detection Range0.02-20ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.007 ng/mL.
- DownloadInstruction Manual
- UOM 8Plex 7Plex 6Plex 5Plex 4Plex 3Plex 2Plex1Plex
- FOB
US$ 427
US$ 443
US$ 468
US$ 501
US$ 534
US$ 583
US$ 657
Result
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Specificity
This assay has high sensitivity and excellent specificity for detection of Caspase 14 (CASP14) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Caspase 14 (CASP14) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Caspase 14 (CASP14) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Caspase 14 (CASP14) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 90-103 | 95 |
EDTA plasma(n=5) | 91-101 | 96 |
heparin plasma(n=5) | 78-102 | 83 |
sodium citrate plasma(n=5) | 94-105 | 101 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Caspase 14 (CASP14) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Caspase 14 (CASP14) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Caspase 14 (CASP14) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 95-105% | 87-94% | 97-105% | 87-97% |
EDTA plasma(n=5) | 81-92% | 90-104% | 80-93% | 78-102% |
heparin plasma(n=5) | 78-96% | 89-99% | 96-104% | 85-94% |
sodium citrate plasma(n=5) | 88-96% | 84-93% | 88-101% | 90-97% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
96-well plate | 1 | Plate sealer for 96 wells | 4 |
Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
Pre-Mixed Magnetic beads (22#:CASP14) | 1 | Analysis buffer | 1×20mL |
Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
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Magazine | Citations |
Journal of Dermatological Science | Pyrrolidone carboxylic acid levels or caspase-14 expression in the corneocytes of lesional skin correlates with clinical severity, skin barrier function and lesional inflammation in atopic dermatitis Pubmed:25315296 |
In Vitro Cell Dev Biol Anim | Luteolin induces caspase-14-mediated terminal differentiation in human epidermal keratinocytes PubMed: 26427706 |
Applied Biochemistry and Biotechnology | Enhanced Induction of Apoptosis in HaCaT Cells by Luteolin Encapsulated in PEGylated Liposomes—Role of Caspase-3/Caspase-14 Pubmed: 30390204 |
Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
RPA457Ra01 | Recombinant Caspase 14 (CASP14) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA457Ra01 | Polyclonal Antibody to Caspase 14 (CASP14) | WB; IHC; ICC; IP. |
MAA457Ra21 | Monoclonal Antibody to Caspase 14 (CASP14) | WB; IHC; ICC; IP. |
SEA457Ra | ELISA Kit for Caspase 14 (CASP14) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA457Ra | Multiplex Assay Kit for Caspase 14 (CASP14) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |