Packages (Simulation)

Polyclonal Antibody to Nischarin (NISCH)

Image (I)

DAB staining on IHC-P; Samples: Mouse Heart Tissue; Primary Ab: 20μg/ml Rabbit Anti-Mouse NISCH Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)

Image (II)

Figure. Western Blot; Sample: Recombinant Nischarin, Mouse.
Western Blot; Sample: Mouse Serum;
Primary Ab: 3µg/ml Rabbit Anti-Mouse NISCH Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Mouse Serum;
Primary Ab: 5µg/ml Rabbit Anti-Mouse NISCH Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

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Instruction Manual

Polyclonal-Antibody-to-Nischarin-(NISCH)-PAA249Mu01.pdf

Certificate

Polyclonal Antibody to Nischarin (NISCH)

IRAS; Imidazoline Receptor Candidate; I-1 Receptor Candidate Protein; Imidazoline Receptor Antisera Selected; Imidazoline receptor antisera-selected protein

Product No.PAA249Mu01
Organism SpeciesMus musculus (Mouse) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPA249Mu01-Recombinant Nischarin (NISCH)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid
Concentration500µg/mL
Organism Species Moren/a
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 97 227 US$ 227 US$ 324 US$ 810 US$ 3240
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against NISCH. It has been selected for its ability to recognize NISCH in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.5-5µg/mL;
Immunohistochemistry: 5-50µg/mL;
Immunocytochemistry: 5-50µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine	Citations
Molecular Medicine Reports	Expression of integrin-binding protein Nischarin in metastatic breast cancer Pubmed:25695373

Catalog No.	Related products for research use of Mus musculus (Mouse) Organism species	Applications (RESEARCH USE ONLY!)
RPA249Mu01	Recombinant Nischarin (NISCH)	Positive Control; Immunogen; SDS-PAGE; WB.
PAA249Mu01	Polyclonal Antibody to Nischarin (NISCH)	WB; IHC; ICC; IP.