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Monoclonal Antibody to Semaphorin 4D (SEMA4D)

CD100; A8; BB18; GR3; M-Sema G; M-Sema-G; SEMAJ; Coll-4; Sema Domain,Immunoglobulin Domain(Ig),Transmembrane Domain(TM)and Short Cytoplasmic Domain 4D

  • Product No.MAB430Hu22
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
  • SourceMonoclonal antibody preparation
  • HostMouse
  • Potency (Clone Number)n/a
  • Ig Isotype IgG
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen n/a
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid
  • Concentration1mg/mL
  • Organism Species Moren/a
  • ApplicationsWB; IHC; ICC; IP.
    If the antibody is used in flow cytometry, please check FCM antibodies.
  • Downloadn/a
  • UOM 20µl100µl 200µl 1ml 10ml
  • FOB US$ 106  US$ 246  US$ 352  US$ 880  US$ 3520 
    For more details, please contact local distributors!

SPECIFITY

The antibody is a mouse monoclonal antibody raised against SEMA4D. It has been selected for its ability to recognize SEMA4D in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.2-2µg/mL;1:500-5000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Expert Opin Ther Targets. Circulating semaphorin-4D and plexin-B1 levels in postmenopausal women with low bone mass: the 3-month effect of zoledronic acid, denosumab or teriparatide treatment Pubmed:25395071
Obesity (Silver Spring). Genome-wide analysis identifies an african-specific variant in SEMA4D associated with body mass index. pubmed:28296344
Clinical Lymphoma, Myeloma & Leukemia A Revised Staging System for Waldenström's Macroglobulinemia 10.1016/j.clml.2017.07.146
Clinical Lymphoma, Myeloma & Leukemia Extramedullary Multiple Myeloma: Clinical Features and Prognosis in Argentina 10.1016/j.clml.2017.07.144
Blood Cancer Journal Semaphorin 4D correlates with increased bone resorption, hypercalcemia, and disease stage in newly diagnosed patients with multiple myeloma Pubmed:29748532
Analytical Biochemistry A high-sensitivity enzyme immunoassay for the quantification of soluble human semaphorin 4D in plasma Pubmed: 30879960
Journal of Allergy and Clinical Immunology-In Practice Pathological and therapeutic implications of eosinophil-derived semaphorin 4D in eosinophilic chronic rhinosinusitis Pubmed: 32035658
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPB430Hu01 Recombinant Semaphorin 4D (SEMA4D) Positive Control; Immunogen; SDS-PAGE; WB.
PAB430Hu01 Polyclonal Antibody to Semaphorin 4D (SEMA4D) WB
MAB430Hu22 Monoclonal Antibody to Semaphorin 4D (SEMA4D) WB; IHC; ICC; IP.
SEB430Hu ELISA Kit for Semaphorin 4D (SEMA4D) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB430Hu Multiplex Assay Kit for Semaphorin 4D (SEMA4D) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.