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Western Blot; Sample: Lane1: Rat Spleen lysate; Lane2: Rat Cerebrum lysate Primary Ab: 2µg/ml Mouse Anti-Human ALOX5 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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Instruction Manual
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Monoclonal Antibody to 5-Lipoxygenase (5-LO)
LOX5; 5-LOX; ALOX-5; ALOX5; 5LPG; LOG5; Lipoxygenase 5
- Product No.MAB355Hu22
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourceMonoclonal antibody preparation
- HostMouse
- Potency (Clone Number)D2
- Ig Isotype IgG2b Kappa
- PurificationProtein A + Protein G affinity chromatography
- LabelNone
- Immunogen RPB355Hu01-Recombinant 5-Lipoxygenase (5-LO)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration1mg/ml
- Organism Species MoreRattus norvegicus (Rat)
- ApplicationsWB.
If the antibody is used in flow cytometry, please check FCM antibodies. - DownloadInstruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
- FOB
US$ 106
US$ 246
US$ 352
US$ 880
US$ 3520
For more details, please contact local distributors!
SPECIFITY
The antibody is a mouse monoclonal antibody raised against 5-LO. It has been selected for its ability to recognize 5-LO in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.01-2µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
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Antibody Labeling Customized Service
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Protein A/G Purification Column
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Staining Solution for Cells and Tissue
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Western Blot (WB) Experiment Service
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Immunohistochemistry (IHC) Experiment Service
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Immunocytochemistry (ICC) Experiment Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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Buffer
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DAB Chromogen Kit
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SABC Kit
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Long-arm Biotin Labeling Kit
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Real Time PCR Experimental Service
| Magazine | Citations |
| Prostaglandins & Other Lipid Mediators | Modulating effect of inositol hexaphosphate on arachidonic acid-dependent pathways in colon cancer cells. pubmed:28797636 |
| Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
| RPB355Hu02 | Recombinant 5-Lipoxygenase (5-LO) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB355Hu01 | Recombinant 5-Lipoxygenase (5-LO) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB355Hu01 | Polyclonal Antibody to 5-Lipoxygenase (5-LO) | WB; IHC; ICC; IP. |
| MAB355Hu22 | Monoclonal Antibody to 5-Lipoxygenase (5-LO) | WB. |
| MAB355Hu25 | Monoclonal Antibody to 5-Lipoxygenase (5-LO) | WB; IHC; ICC; IP. |
| MAB355Hu21 | Monoclonal Antibody to 5-Lipoxygenase (5-LO) | WB |
| MAB355Hu27 | Monoclonal Antibody to 5-Lipoxygenase (5-LO) | WB; IHC; ICC; IP. |
| MAB355Hu29 | Monoclonal Antibody to 5-Lipoxygenase (5-LO) | WB; IHC; ICC; IP. |
| MAB355Hu23 | Monoclonal Antibody to 5-Lipoxygenase (5-LO) | WB; IHC; ICC; IP. |
| MAB355Hu24 | Monoclonal Antibody to 5-Lipoxygenase (5-LO) | WB; IHC; ICC; IP. |
| SEB355Hu | ELISA Kit for 5-Lipoxygenase (5-LO) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB355Hu | Multiplex Assay Kit for 5-Lipoxygenase (5-LO) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |