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Human Hela cell line was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 10µg/ml mouse Anti-human MANF Monoclonal Antibody (Catalog FAC300Hu04, red histogram) or Isotype control antibody (Catalog IS067-Mu03, green histogram), followed by 1µg/ml FITC-conjugated Anti-mouse IgG Secondary Antibody (Catalog SAA544Mu18).
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Anti-Mesencephalic Astrocyte Derived Neurotrophic Factor (MANF) Monoclonal Antibody
ARMET; ARP; Arginine Rich Mutated In Early Stage Tumors; Arginine-rich protein
- Product No.FAC300Hu04
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Potency (Clone Number)F4
- Ig Isotype IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Excitation Laser
- Vol. per test
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid
- Organism Species Moren/a
- ApplicationsFCM
- Downloadn/a
- UOM 25test50test 100test 200test 500test
- FOB
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For more details, please contact local distributors!
USAGE
Flow cytometry: 10µg/ml;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
GIVEAWAYS
INCREMENT SERVICES
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Protein A/G Purification Column
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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Buffer
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DAB Chromogen Kit
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SABC Kit
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Isotype Control
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Real Time PCR Experimental Service
| Magazine | Citations |
| PLoS One. | Molecular Effects of Doxycycline Treatment on Pterygium as Revealed by Massive Transcriptome Sequencing PubMed: PMC3378547 |
| Endocr J. | Circulating mesencephalic astrocyte-derived neurotrophic factor is increased in newly diagnosed prediabetic and diabetic patients, and is associated with insulin resistance. pubmed:28216543 |
| Journal of Steroid Biochemistry and Molecular Biology | Depressive-like effect of prenatal exposure to DDT involves global DNA hypomethylation and impairment of GPER1/ESR1 protein levels but not ESR2 and AHR/ARNT signaling pubmed:28263910 |
| Endocrine Journal | Circulating mesencephalic astrocyte-derived neurotrophic factor is increased in newly diagnosed prediabetic and diabetic patients, and is associated with insulin resistance 10.1507:endocrj.EJ16-0472 |
| Nature Metabolism | MANF regulates metabolic and immune homeostasis in ageing and protects against liver damage |
| J Exp Med | Feeding-induced hepatokine, Manf, ameliorates diet-induced obesity by promoting adipose browning via p38 MAPK pathway 33856409 |
| J Inflamm Res | The Potential Protective Effect of Mesencephalic Astrocyte-Derived Neurotrophic Factor on Post-Operative Delirium via Inhibiting Inflammation and Microglia … 34234505 |