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Image (II)
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Human Hela cell line was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-rat MK Polyclonal Antibody (Catalog FAA631Ra01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
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Anti-Midkine (MK) Polyclonal Antibody
MDK; ARAP; NEGF2; Neurite Growth-Promoting Factor 2; Amphiregulin-associated protein; Midgestation and kidney protein; Neurite outgrowth-promoting factor 2
- Product No.FAA631Ra01
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Potency (Clone Number)n/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Excitation Laser
- Vol. per test
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid
- Organism Species MoreHomo sapiens (Human)
- ApplicationsFCM
- Downloadn/a
- UOM 25test50test 100test 200test 500test
- FOB
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For more details, please contact local distributors!
USAGE
Flow cytometry: 20µg/ml;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
GIVEAWAYS
INCREMENT SERVICES
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Protein A/G Purification Column
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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Buffer
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DAB Chromogen Kit
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SABC Kit
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Isotype Control
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Real Time PCR Experimental Service
| Magazine | Citations |
| Midkine: From Embryogenesis to Pathogenesis and Therapy | Immunochemical Methods for the Detection of Midkine in Samples of Human Origin Springer: Source |
| 33 | Midkine Controls Arteriogenesis by Regulating the Bioavailability of Vascular Endothelial Growth Factor A and the Expression of Nitric Oxide Synthase 1 and … 10.1016:j.ebiom.2017.11.020 |
| Circulation Journal | Midkine Promotes Atherosclerotic Plaque Formation Through Its Pro-Inflammatory, Angiogenic and Anti-Apoptotic Functions in Apolipoprotein E-Knockout Mice pubmed:28781288 |
| EBioMedicine | Midkine Controls Arteriogenesis by Regulating the Bioavailability of Vascular Endothelial Growth Factor A and the Expression of Nitric Oxide Synthase 1 and … Pubmed:29233575 |
| Journal of Clinical Laboratory Analysis | Clinical value of jointly detection pleural fluid Midkine, pleural fluid adenosine deaminase, and pleural fluid carbohydrate antigen 125 in the identification of nonsmall … Pubmed:29797475 |
| Charakterisierung der funktionalen Rolle des Zytokins Midkine bei der Arteriogenese : | |
| Scientific Reports | The possible role of Dickkopf-1, Golgi protein-73 and Midkine as predictors of hepatocarcinogenesis: a review and an Egyptian study Pubmed: 32198440 |
| Odontology | Effects of different pulp-capping materials on cell death signaling pathways of lipoteichoic acid-stimulated human dental pulp stem cells Pubmed: 33206337 |
| Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
| RPA631Ra01 | Recombinant Midkine (MK) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA631Ra01 | Polyclonal Antibody to Midkine (MK) | WB |
| FAA631Ra01 | Anti-Midkine (MK) Polyclonal Antibody | FCM |
| LAA631Ra71 | Biotin-Linked Polyclonal Antibody to Midkine (MK) | WB |
| MAA631Ra21 | Monoclonal Antibody to Midkine (MK) | WB; IHC; ICC; IP. |
| SEA631Ra | ELISA Kit for Midkine (MK) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA631Ra | Multiplex Assay Kit for Midkine (MK) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |