ELISA Kit for Ephrin B2 (EFNB2)

EPLG5; HTKL; Htk-L; LERK5; HTK Ligand; Ligand Of Eph-Related Kinase 5; Eph-Related Receptor Tyrosine Kinase Ligand 5

Specificity

This assay has high sensitivity and excellent specificity for detection of Ephrin B2 (EFNB2).
No significant cross-reactivity or interference between Ephrin B2 (EFNB2) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ephrin B2 (EFNB2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ephrin B2 (EFNB2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

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Magazine Citations
ATS Journals Soluble Ephrin-B2 is a Novel Biomarker for Idiopathic Pulmonary Fibrosis Doi: Pdf
D50. PEDIATRIC RARE LUNG DISEASES Soluble Ephrin-B2 Is A Novel Biomarker Of Pulmonary Fibrosis doi:A7217
An International Journal of Medicine  P055 Over-expression of IL-6 and OSM impacts the polarization of pro-fibrotic macrophages and the development of bleomycin-induced lung fibrosis content:109
An International Journal of Medicine P057 Soluble Ephrin-B2 is a Prognostic Biomarker of Pulmonary Fibrosis S40.2.extract
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