CLIA Kit for Mucin 5 Subtype AC (MUC5AC)
TBM; MUC5; LeB; Oligomeric Mucus/Gel-Forming; Mucin 5,Subtypes A And C,Tracheobronchial/Gastric; Gastric mucin; Lewis B blood group antigen; Major airway glycoprotein
- Product No.SCA756Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length2h, 40min
- Detection Range13.7-10,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 5.3pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 864
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For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Mucin 5 Subtype AC (MUC5AC).
No significant cross-reactivity or interference between Mucin 5 Subtype AC (MUC5AC) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Mucin 5 Subtype AC (MUC5AC) and the recovery rates were calculated by comparing the measured value to the expected amount of Mucin 5 Subtype AC (MUC5AC) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mucin 5 Subtype AC (MUC5AC) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mucin 5 Subtype AC (MUC5AC) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mucin 5 Subtype AC (MUC5AC) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|Substrate A||1×10mL||Substrate B||1×2mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.
|Annals of surgical oncology||Pancreatic cyst fluid and serum mucin levels predict dysplasia in intraductal papillary mucinous neoplasms of the pancreas. PubMed: 20717734|
|Investigative Ophthalmology & Visual Science||Entrapment of Conjunctival Goblet Cells by Desiccation-Induced Cornification PubMed: 21421863|
|Cell & Molecular Biology||Role of aquaporin 5 in antigen-induced airway inflammation and mucous hyperproduction in mice PubMed: 20550619|
|Mucosal Immunology||Homeostatic control of conjunctival mucosal goblet cells by NKT-derived IL-13 Nature: 201082a|
|European Journal of Pharmacology||Naringin attenuates EGF-induced MUC5AC secretion in A549 cells by suppressing the cooperative activities of MAPKs-AP-1 and IKKs-IκB-NF-κB signaling pathways ScienceDirect: S0014299912005523|
|PLoS One||Baicalein Reduces Airway Injury in Allergen and IL-13 Induced Airway Inflammation PubMed: PMC3639905|
|Respiratory Researh||Pyocyanin-induced mucin production is associated with redox modification of FOXA2 PubMed: PMC3765780|
|Surgery||A novel serum marker for biliary tract cancer: Diagnostic and prognostic values of quantitative evaluation of serum mucin 5AC (MUC5AC) Pubmed: 24468034|
|Biochemical and Biophysical Research Communications||The human cathelicidin LL-37 enhances airway mucus production in chronic obstructive pulmonary disease Pubmed:24291709|
|Journal of Proteomics||Proteomic analysis of cancer and mesothelial cells reveals an increase in Mucin 5AC during ovarian cancer and peritoneal interaction Pubmed:24726482|
|Experimental Lung Research||The human Cathelicidin LL-37 induces MUC5AC mucin production by airway epithelial cells via TACE-TGF-α-EGFR pathway Pubmed:24901072|
|Surgery.?||Assessment of bile and serum mucin5AC in cholangiocarcinoma: Diagnostic performance and biologic significance Pubmed:25151557|
|Allergology International||Leukotriene E 4 induces MUC5AC release from human airway epithelial NCI-H292 cells Pubmed:25838093|
|Respirology||Secreted mucins and airway bacterial colonization in non-CF bronchiectasis PubMed: 26172851|
|S||Isabela Casagrande Jeremias Teses: Disponiveis|
|American Thoracic Society International Conference Abstracts||Mucin Secretion In Asthmatics With Osmotic Airway Hyperresponsiveness doi:10.1164|
|Deletion Polymorphism and Chronic Obstructive Pulmonary Disease||ReCavia (Guinea pig )lation of Viral Infection-induced Airway Remodeling Cytokine Production by the TLR3-EGFR Signaling Pathway in Human Bronchial Epithelial Cells Pubmed:27075970|
|Int J Clin Exp Med||The influence of long-term corneal contact lens wearing on the stability and quality of tear film in Qinghai files:ijcem0020163.pdf|
|Ann Am Thorac Soc||Airway Mucin 2 Is Decreased in Patients with Severe Chronic Obstructive Pulmonary Disease with Bacterial Colonization doi:10.1513|
|Sci Rep||Impact of Cigarette Smoking on Tear Function and Correlation between Conjunctival Goblet Cells and Tear MUC5AC Concentration in Office Workers Pubmed:27297822|
|Cytokine.||Interleukin-33 induces mucin gene expression and goblet cell hyperplasia in human nasalepithelial cells. pubmed:27776277|
|J Virol.||Mutating the CX3C Motif in the G Protein Should Make a Live Respiratory Syncytial Virus Vaccine Safer and More Effective. pubmed:28275196|
|Respiratory Physiology & Neurobiology||Peroxiredoxin 6 suppresses Muc5ac overproduction in LPS-induced airway inflammation through H2O2-EGFR-MAPK signaling pathway. pubmed:27884794|
|Journal of Immunology Research||Beneficial effects of prebiotic Saccharomyces cerevisiae mannan on allergic asthma mouse models pubmed:28835901|
|BASIC INVESTIGATION||Efficacy of Sodium Hyaluronate in Murine Diabetic Ocular Surface Diseases pubmed:28644234|
|International Immunopharmacology||Clarithromycin inhibits TNF-α-induced MUC5AC mucin gene expression via the MKP-1-p38MAPK-dependent pathway pubmed:28550735|
|Секреторно-экссудативная реакция слизистой носа на воздействие холодного воздуха у больных бронхиальной астмой 10.20538/1682-0363-2017-2-146-158|
|Cellular Microbiology||An adherent mucus layer attenuates the genotoxic effect of colibactin. pubmed:29156489|
|Respiratory Research||IL-4-induced caveolin-1-containing lipid rafts aggregation contributes to MUC5AC synthesis in bronchial epithelial cells pubmed:28931396|
|Chemotherapy||Oxytetracycline Inhibits Mucus Secretion and Inflammation in Human Airway Epithelial Cells. pubmed:28578323|
|Allergy||Th2 cytokines orchestrate the secretion of MUC 5 AC and MUC 5B in IL‐5 positive chronic rhinosinusitis with nasal polyps Pubmed:29802623|
|European Journal of Pharmacology||Salvianolic acid B improves airway hyperresponsiveness by inhibiting MUC5AC overproduction associated with Erk1/2/P38 signaling Pubmed:29382534|
|Inflammation||Plantamajoside Inhibits Lipopolysaccharide-Induced MUC5AC Expression and Inflammation through Suppressing the PI3K/Akt and NF-κB Signaling Pathways in … Pubmed:29349683|
|Pulmonary Pharmacology and Therapeutics||In vivo and in vitro investigation of anti-inflammatory and mucus-regulatory activities of a fixed combination of thyme and primula extracts Pubmed:29698781|
|European Respiratory Society||Profiling of healthy and asthmatic airway smooth muscle cells following IL-1β treatment: a novel role for CCL20 in chronic mucus hyper-secretion Pubmed:29946002|
|Catalog No.||Related products for research use of Mus musculus (Mouse) Organism species||Applications (RESEARCH USE ONLY!)|
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