ELISA Kit DIY Materials for Mucin 5 Subtype AC (MUC5AC)

TBM; MUC5; LeB; Oligomeric Mucus/Gel-Forming; Mucin 5,Subtypes A And C,Tracheobronchial/Gastric; Gastric mucin; Lewis B blood group antigen; Major airway glycoprotein

  • Product No.KSA756Mu01
  • Organism SpeciesMus musculus (Mouse) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.156-10ng/mL
  • Applicable Sensitivity0.064ng/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1166  US$ 1944  US$ 3499  US$ 6804  US$ 11664 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Mucin 5 Subtype AC (MUC5AC). No significant cross-reactivity or interference between Mucin 5 Subtype AC (MUC5AC) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Annals of surgical oncology Pancreatic cyst fluid and serum mucin levels predict dysplasia in intraductal papillary mucinous neoplasms of the pancreas. PubMed: 20717734
Investigative Ophthalmology & Visual Science Entrapment of Conjunctival Goblet Cells by Desiccation-Induced Cornification PubMed: 21421863
Cell & Molecular Biology Role of aquaporin 5 in antigen-induced airway inflammation and mucous hyperproduction in mice PubMed: 20550619
Mucosal Immunology Homeostatic control of conjunctival mucosal goblet cells by NKT-derived IL-13 Nature: 201082a
European Journal of Pharmacology Naringin attenuates EGF-induced MUC5AC secretion in A549 cells by suppressing the cooperative activities of MAPKs-AP-1 and IKKs-IκB-NF-κB signaling pathways ScienceDirect: S0014299912005523
PLoS One Baicalein Reduces Airway Injury in Allergen and IL-13 Induced Airway Inflammation PubMed: PMC3639905
Respiratory Researh Pyocyanin-induced mucin production is associated with redox modification of FOXA2 PubMed: PMC3765780
Surgery A novel serum marker for biliary tract cancer: Diagnostic and prognostic values of quantitative evaluation of serum mucin 5AC (MUC5AC) Pubmed: 24468034
Biochemical and Biophysical Research Communications The human cathelicidin LL-37 enhances airway mucus production in chronic obstructive pulmonary disease Pubmed:24291709
Journal of Proteomics Proteomic analysis of cancer and mesothelial cells reveals an increase in Mucin 5AC during ovarian cancer and peritoneal interaction Pubmed:24726482
Experimental Lung Research The human Cathelicidin LL-37 induces MUC5AC mucin production by airway epithelial cells via TACE-TGF-α-EGFR pathway Pubmed:24901072
Surgery.? Assessment of bile and serum mucin5AC in cholangiocarcinoma: Diagnostic performance and biologic significance Pubmed:25151557
Allergology International Leukotriene E 4 induces MUC5AC release from human airway epithelial NCI-H292 cells Pubmed:25838093
Respirology Secreted mucins and airway bacterial colonization in non-CF bronchiectasis PubMed: 26172851
S Isabela Casagrande Jeremias Teses: Disponiveis
American Thoracic Society International Conference Abstracts Mucin Secretion In Asthmatics With Osmotic Airway Hyperresponsiveness doi:10.1164
Deletion Polymorphism and Chronic Obstructive Pulmonary Disease ReCavia (Guinea pig )lation of Viral Infection-induced Airway Remodeling Cytokine Production by the TLR3-EGFR Signaling Pathway in Human Bronchial Epithelial Cells Pubmed:27075970
Int J Clin Exp Med The influence of long-term corneal contact lens wearing on the stability and quality of tear film in Qinghai files:ijcem0020163.pdf
Ann Am Thorac Soc Airway Mucin 2 Is Decreased in Patients with Severe Chronic Obstructive Pulmonary Disease with Bacterial Colonization doi:10.1513
Sci Rep Impact of Cigarette Smoking on Tear Function and Correlation between Conjunctival Goblet Cells and Tear MUC5AC Concentration in Office Workers Pubmed:27297822
Cytokine. Interleukin-33 induces mucin gene expression and goblet cell hyperplasia in human nasalepithelial cells. pubmed:27776277
J Virol.  Mutating the CX3C Motif in the G Protein Should Make a Live Respiratory Syncytial Virus Vaccine Safer and More Effective. pubmed:28275196
Respiratory Physiology & Neurobiology Peroxiredoxin 6 suppresses Muc5ac overproduction in LPS-induced airway inflammation through H2O2-EGFR-MAPK signaling pathway. pubmed:27884794
Journal of Immunology Research Beneficial effects of prebiotic Saccharomyces cerevisiae mannan on allergic asthma mouse models pubmed:28835901
BASIC INVESTIGATION Efficacy of Sodium Hyaluronate in Murine Diabetic Ocular Surface Diseases pubmed:28644234
International Immunopharmacology Clarithromycin inhibits TNF-α-induced MUC5AC mucin gene expression via the MKP-1-p38MAPK-dependent pathway pubmed:28550735
Секреторно-экссудативная реакция слизистой носа на воздействие холодного воздуха у больных бронхиальной астмой 10.20538/1682-0363-2017-2-146-158
Cellular Microbiology An adherent mucus layer attenuates the genotoxic effect of colibactin. pubmed:29156489
Respiratory Research IL-4-induced caveolin-1-containing lipid rafts aggregation contributes to MUC5AC synthesis in bronchial epithelial cells pubmed:28931396
Chemotherapy Oxytetracycline Inhibits Mucus Secretion and Inflammation in Human Airway Epithelial Cells. pubmed:28578323
Allergy Th2 cytokines orchestrate the secretion of MUC 5 AC and MUC 5B in IL‐5 positive chronic rhinosinusitis with nasal polyps Pubmed:29802623
European Journal of Pharmacology  Salvianolic acid B improves airway hyperresponsiveness by inhibiting MUC5AC overproduction associated with Erk1/2/P38 signaling Pubmed:29382534
Inflammation Plantamajoside Inhibits Lipopolysaccharide-Induced MUC5AC Expression and Inflammation through Suppressing the PI3K/Akt and NF-κB Signaling Pathways in … Pubmed:29349683
Pulmonary Pharmacology and Therapeutics In vivo and in vitro investigation of anti-inflammatory and mucus-regulatory activities of a fixed combination of thyme and primula extracts Pubmed:29698781
European Respiratory Society Profiling of healthy and asthmatic airway smooth muscle cells following IL-1β treatment: a novel role for CCL20 in chronic mucus hyper-secretion Pubmed:29946002
Clinical and Experimental Ophthalmology Development of a highly sensitive and reliable enzyme-linked immunosorbent assay for MUC5aC in human tears extracted from schirmer strips Pubmed: 30214141
international journal of biological macromolecules Increased expression of long non-coding RNA SNHG16 correlates with tumor progression and poor prognosis in non-small cell lung cancer Pubmed: 30287374
Journal of Investigative Medicine The characteristics and clinical significance of mucin levels in bronchoalveolar lavage fluid of patients with interstitial lung disease Pubmed: 30573494
Phytomedicine Regulation effects of naringin on diesel particulate matter-induced abnormal airway surface liquid secretion Pubmed: 31301536
Scientific Reports Regional comparison of Goblet cell number and Area in exposed and covered Dry eyes and their Correlation with Tear MUC5AC Pubmed: 32076085
Eur J Pharmacol TLR3 inhibitor and tyrosine kinase inhibitor attenuate cigarette smoke/poly I: C-induced airway inflammation and remodeling by the EGFR/TLR3/MAPK … Pubmed: 33068589
journal of ethnopharmacology Effective-component compatibility of Bufei Yishen formula II inhibits mucus hypersecretion of chronic obstructive pulmonary disease rats by regulating EGFR/PI3K … Pubmed: 32344236
Less Airway Inflammation and Goblet Cell Metaplasia in an IL-33-Induced Asthma Model of Leptin-Deficient Obese Mice
Exp Mol Pathol The regulatory role of SFRP5/WNT5A axis in allergic rhinitis through inhibiting JNK pathway activation and lowering mucin generation in human nasal?¡­ 33285209
FEBS Open Bio Porphyromonas gingivalis gingipains potentially affect MUC5AC gene expression and protein levels in respiratory epithelial cells 33332733
Cell Rep Generation of functional conjunctival epithelium, including goblet cells, from human iPSCs 33535050
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KSA756Mu01 ELISA Kit DIY Materials for Mucin 5 Subtype AC (MUC5AC) Main materials for "Do It (ELISA Kit) Yourself".