Packages (Simulation)

Image (I)

DAB staining on IHC-P; Sample: Human Small intestine Tissue; Primary Ab: 20µg/ml Mouse Anti-Human CFL1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Liver Tissue;
Primary Ab: 20µg/ml Mouse Anti-Human CFL1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Spleen Tissue;
Primary Ab: 20µg/ml Mouse Anti-Human CFL1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Small intestine Tissue
Primary Ab: 20µg/ml Mouse Anti-Human CFL1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Lung Tissue
Primary Ab: 20µg/ml Mouse Anti-Human CFL1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Spleen Tissue
Primary Ab: 20µg/ml Mouse Anti-Human CFL1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
AF488 staining on IF;
Sample: Human Hela cell;
Primary Ab: 30µg/ml Mouse Anti-Human CFL1 Antibody
Second Ab: 2µg/ml AF488-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu11)
FITC staining on IF;
Sample: Hela cell
Primary Ab: 30µg/ml Mouse Anti-Human CFL1 Antibody
Second Ab: 2µg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu11)

Image (II)

Western Blot; Sample: Lane1: Porcine Cerebrum lysate; Lane2: Porcine Liver lysate Primary Ab: 2µg/ml Mouse Anti-Human CFL1 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
Western Blot; Sample: Lane1: Bovine Cerebrum lysate; Lane2: HepG2 cell lysate
Primary Ab: 0.2µg/ml Mouse Anti-Human CFL1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)

Quality Guarantee

Instruction Manual

Monoclonal-Antibody-to-Cofilin-1-(CFL1)-MAB559Hu22.pdf

Certificate

Monoclonal Antibody to Cofilin 1 (CFL1)

CFL; p18; N-Cofilin; 18 kDa phosphoprotein; Cofilin 1, Non Muscle

Product No.MAB559Hu22
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourceMonoclonal antibody preparation
HostMouse
Potency (Clone Number)C12
Ig Isotype IgG2a Kappa
PurificationProtein A + Protein G affinity chromatography
LabelNone
Immunogen RPB559Hu01-Recombinant Cofilin 1 (CFL1)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid
Concentration1mg/ml
Organism Species MoreSus scrofa; Porcine (Pig), Bos taurus; Bovine (Cattle)
ApplicationsWB; IHC; ICC/IF
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 106 246 US$ 246 US$ 352 US$ 880 US$ 3520
For more details, please contact local distributors!

SPECIFITY

The antibody is a mouse monoclonal antibody raised against CFL1. It has been selected for its ability to recognize CFL1 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-30µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Magazine	Citations
Brazilian Journal of Medical and Biological Research	Detection of sputum cofilin-1 as indicator of malignancy Pubmed:29846436
Nutrients	Hydroxytyrosol as a Promising Ally in the Treatment of Fibromyalgia Pubmed: 32784915
	The level of cytoskeleton remodeling proteins in the blood serum and the expression of their mRNA in the tumor tissue in metastasis of the larynx and hypopharynx cancer

Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
RPB559Hu01	Recombinant Cofilin 1 (CFL1)	Positive Control; Immunogen; SDS-PAGE; WB.
APB559Hu01	Active Cofilin 1 (CFL1)	Cell culture; Activity Assays.
PAB559Hu01	Polyclonal Antibody to Cofilin 1 (CFL1)	WB; IHC; ICC; IP.
FAB559Hu51	APC-Linked Anti-Cofilin 1 (CFL1) Polyclonal Antibody	FCM
MAB559Hu21	Monoclonal Antibody to Cofilin 1 (CFL1)	WB; IHC; ICC; IP.
MAB559Hu22	Monoclonal Antibody to Cofilin 1 (CFL1)	WB; IHC; ICC/IF
MAB559Hu23	Monoclonal Antibody to Cofilin 1 (CFL1)	WB; IHC; ICC; IP.
MAB559Hu27	Monoclonal Antibody to Cofilin 1 (CFL1)	WB; IHC; ICC; IP.
MAB559Hu24	Monoclonal Antibody to Cofilin 1 (CFL1)	WB; IHC; ICC; IP.
MAB559Hu25	Monoclonal Antibody to Cofilin 1 (CFL1)	WB; IHC; ICC; IP.
SEB559Hu	ELISA Kit for Cofilin 1 (CFL1)	Enzyme-linked immunosorbent assay for Antigen Detection.
SCB559Hu	CLIA Kit for Cofilin 1 (CFL1)	Chemiluminescent immunoassay for Antigen Detection.
LMB559Hu	Multiplex Assay Kit for Cofilin 1 (CFL1) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.